Data/hora: 16/06/2024 10:45:17

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 11/04/2001
Data da última atualização: | 11/04/2001
Autoria: | GIANINAZZI-PEARSON, V.; ARNOULD, C.; OUFATTOLE, M.; ARANGO, M.; GIANINAZZI, S.
Título: | Differential activation of H+ _ATPase genes by an arbuscular mycorrhizal fungus in root cells of transgenic tobacco.
Ano de publicação: | 2000
Fonte/Imprenta: | Panta, v.2 11, p.609-613, 2000.
Idioma: | Inglês
Conteúdo: | In arbuscular mycorrhizas, H+ -ATPase is active in the plant membrane around arbuscules but absent from plant mutants defective in arbuscule development (Gianinazzi-Pearson et al. 1995, Can. J. Bot. 73:S526-S532). The proton-pumping H+ -ATPase is encoded by a family of genes in plants. Immunocytochemical studies and promoter-gus A fusion assays were performed in transgenic tobacco (Nicotiana tabacum L.) to determine whether the periarbuscular enzyme activity results from de-novo activation of plant genes by an arbuscular mycorrhizal fungus. The H+ -ATPase protein was localized in the plant membrane around arbuscule hyphae. The enzyme was absent from non-colonized cortical cells. Regulation of seven H+ -ATPase genes (pma) was compared in non-mycorrhizal and mycorrhizal roots by histochemical detection of B-glucuronidase (GUS) activity. Two genes (pma2, pma4) were induced in arbuscule-containing cells of mycorrhizal roots but not in non-mycorrhizal cortical tissues or senescent mycorrhiza. It is concluded that de-novo H+ -ATPase activity in the periarbuscular membrane results from selective induction of two H+ -ATPase genes, which can have diverse roles in plant-fungal interactions at the symbiotic interface.
Thesagro: | Fumo; Fungo; Micorriza Vesicular Arbuscular; Raiz; Simbiose.
Thesaurus Nal: | fungi; roots; symbiosis; tobacco; vesicular arbuscular mycorrhizae.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24586-1) - ADD - SP - -- -  -  - 22397 - DIS