Data/hora: 09/06/2024 06:03:52

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	18/03/1996	
Data da última atualização: 	28/11/2023	
Autoria: 	DIAZ-APARICIO, E.; MARIN, C.; ALONSO-URMENETA, B.; ARAGON, V.; PEREZ-ORTIZ, S.; PARDO, M.; BLASCO, J. M.; DIAZ, R.; MORIYON, I.	
Título: 	Evaluation of serological tests for diagnosis of Brucells melitensis infection of goats.	
Ano de publicação: 	1994	
Fonte/Imprenta: 	Journal of Clinical Microbiology, v. 32, n. 5, p. 1159-1165, May, 1994.	
DOI: 	10.1128/jcm.32.5.1159-1165.1994	
Idioma: 	Inglês	
Conteúdo: 	Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctivally (10(9) CFU of B. melitensis Rev 1) all tests were 100% specific 4 months after vaccination. The proportion of goats giving a positive reaction after vaccination decreased faster in RID than in other tests.	
Palavras-Chave: 	Dianostico; Sensitivity and specificity; Serologic Test methods.	
Thesagro: 	Brucelose; Caprino; Doença.	
Thesaurus Nal: 	Antigens; Brucellosis; Disease control; Disease diagnosis; Disease prevention; Goat diseases; Goats; Immunology; Pharmacology; Rose bengal; Vaccination.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (9525-1) - ADD - AP - DD -  -  -  - DIS