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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/09/1993 |
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Data da última atualização: |
09/09/1993 |
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Autoria: |
MORENO, P.; GUERRI, J.; BALLESTER-OLMOS, J. F.; MARTINEZ, M. E. |
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Afiliação: |
Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain. |
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Título: |
Segregation of citrus tristeza virus strains evidenced by double stranded RNA (dsRNA) analysis |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Riverside, California: International Organization of Citrus Virologists, 1991. |
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Páginas: |
p.20-23 |
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Idioma: |
Inglês |
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Notas: |
Conference of the International Organization of citrus virologists, 11., 1991, Riverside California, Proceedings... |
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Conteúdo: |
A field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus ans virus-like diseaes by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an inconspicuous stem pitting on Mexican lime. Two months after aphid transmission, one of the infected lime seedlings was used as an inoculum source to graft-inoculate 40 citrom plants.Two years later, the citron plants were analysed individually for dsNRA content and up to 23 different electrophoretic profiles were found. DsNRA analysis has been repeated three times through the year and the individual patterns remained unchanged. Thirteen of these CTV sub-isolates weregraft-transmitted to sweet orange seedlings and the corresponding dsNRA profiles were identical to those previously obtained from citron. All these subsolates induced mild symptoms of Mexican lime similar to those of the original isolate (T-385). These results substantiate that several CTV strains can infect a single citrus plant. |
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Palavras-Chave: |
Silver stain; Symptoms. |
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Thesaurus Nal: |
electrophoresis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01835naa a2200217 a 4500
001 1644213
005 1993-09-09
008 1991 bl uuuu u00u1 u #d
100 1 $aMORENO, P.
245 $aSegregation of citrus tristeza virus strains evidenced by double stranded RNA (dsRNA) analysis
260 $c1991
300 $ap.20-23
500 $aConference of the International Organization of citrus virologists, 11., 1991, Riverside California, Proceedings...
520 $aA field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus ans virus-like diseaes by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an inconspicuous stem pitting on Mexican lime. Two months after aphid transmission, one of the infected lime seedlings was used as an inoculum source to graft-inoculate 40 citrom plants.Two years later, the citron plants were analysed individually for dsNRA content and up to 23 different electrophoretic profiles were found. DsNRA analysis has been repeated three times through the year and the individual patterns remained unchanged. Thirteen of these CTV sub-isolates weregraft-transmitted to sweet orange seedlings and the corresponding dsNRA profiles were identical to those previously obtained from citron. All these subsolates induced mild symptoms of Mexican lime similar to those of the original isolate (T-385). These results substantiate that several CTV strains can infect a single citrus plant.
650 $aelectrophoresis
653 $aSilver stain
653 $aSymptoms
700 1 $aGUERRI, J.
700 1 $aBALLESTER-OLMOS, J. F.
700 1 $aMARTINEZ, M. E.
773 $tRiverside, California: International Organization of Citrus Virologists, 1991.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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