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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/10/2019 |
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Data da última atualização: |
22/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, S. A. S. de; FERREIRA, C. F.; DIAMANTINO, M. S. A. S.; SANTOS, T. A.; PEREIRA, J. dos S.; OLIVEIRA, E. J. de. |
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Afiliação: |
SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; MARIA SELMA ALVES SILVA DIAMANTINO; TAÍS ARAÚJO SANTOS, UFRB; JOCILENE DOS SANTOS PEREIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
First report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Plant Pathology, August, 2019. |
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ISSN: |
1125-4653 |
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DOI: |
https://doi.org/10.1007/s42161-019-00384-6 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
Cassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil. MenosCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV ... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
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Marc: |
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024 7 $ahttps://doi.org/10.1007/s42161-019-00384-6$2DOI
100 1 $aOLIVEIRA, S. A. S. de
245 $aFirst report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil.$h[electronic resource]
260 $c2019
500 $aOnline first.
520 $aCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil.
650 $aMandioca
700 1 $aFERREIRA, C. F.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aSANTOS, T. A.
700 1 $aPEREIRA, J. dos S.
700 1 $aOLIVEIRA, E. J. de
773 $tJournal of Plant Pathology, August, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 4. |  | SANTOS, J. B. F.; FRANCO, M. M.; ANTUNES, R. C.; GUIMARÃES, E. C.; MUNDIM, A. V. Serum biochemical profile of Pêga breed donkeys in the state of Minas Gerais. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1225-1231, junho 2018 Título em português: Perfil bioquímico sérico de jumentos da raça Pêga no estado de Minas Gerais.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 7. | | ESTEVÃO, L. R. de M.; MEDEIROS, J. P. de; SCOGNAMILLO-SZABÓ, M.V.R.; BARATELLA-EVÊNCIO, L.; GUIMARÃES, E. C.; CÂMARA, C. A. G. da; EVÊNCIO-NETO, J. Neoangiogênese de retalhos cutâneos em ratos tratados com óleo de copaíba. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 4, p. 406-412, abr. 2009 Título em inglês: Neoangiogenesis of skin fl aps in rats treated with copaiba oil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | RODRIGUES, T. O.; SANTOS, A. L. Q.; PEREIRA, P. V. R.; SIQUEIRA, S. E.; RODRIGUES, T. C. S.; GUIMARÃES, E. C. Uso de cetamina pela via cólon-retal para contenção química de jiboias Boa constrictor Linnaeus, 1758 com teste de influência da droga sobre o sistema renal. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 2, p. 328-334, fevereiro 2018 Título em inglês: Use of ketamine by colon-rectal route for chemical restraint in Boa constrictor Linnaeus, 1758 with test of influence of the drug on renal system.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | FERREIRA, I. M.; BRAGA, T. F.; GUIMARÃES, E. C.; DURVAL, M. C.; MENDES, L. B.; SOARES, J. S.; MATARIM, D. L.; KRUGER, B. C.; FRANCO, M. M.; ANTUNES, R. C. A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs. Genetics and Molecular Research, v. 18, n. 2, article gmr18257, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | TIMM, L. C.; RECKZIEGEL, N. L.; AQUINO, L. S.; BASSOI, L. H.; GUIMARÃES, E. C.; TAVARES, V. E. Q.; VAZ, C. M. P.; MANIERI, J. M.; RAMOS, C. M. C.; RIBEIRO, P. R. de A.; REICHARDT, K. Spatial variability of physical and hydraulic attributes of a sandy soil in the Northeast Brazil. Revista Suelos Ecuatoriales, Colombia, v. 40, n. 2, p. 139-151, 2010.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | TIMM, L. C.; RECKZIEGEL, N. L.; AQUINO, L. S.; BASSOI, L. H.; GUIMARÃES, E. C.; TAVARES, V. E. Q.; VAZ, C. M. P.; MANIERI, J. M.; RAMOS, C. M. C.; RIBEIRO, P. R. de A.; REICHARDT, K. Spatial variability of physical and hydraulic attributes of a sandy soil in the northeast brazil. Suelos Ecuatoriales, Medelin, v. 40, n. 2, p. 139-151, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 11 | |
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