Data/hora: 04/06/2024 18:01:38

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 08/02/2018
Data da última atualização: | 08/02/2018
Autoria: | FERREIRA JUNIOR, O. L.; OLIVEIRA, R. D. de; MELO, I. S. de; MORAES, L. A. B.
Afiliação: | O. L. FERREIRA JUNIOR; R. D. de OLIVEIRA; ITAMAR SOARES DE MELO, CNPMA; L. A. B. MORAES.
Título: | Effects of different culture medium on albocycline and analogous production by Streptomyces sp. from caatinga biome.
Ano de publicação: | 2017
Fonte/Imprenta: | In: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES, 2017.
Idioma: | Inglês
Conteúdo: | The Albocycline (Fig. A), a macrocyclic lactone knowing by their antimicrobial activity, however it is poorly explored by its biological activity, for example fitotoxic activity. Despite the urgency for discoveries in the natural pesticides and agro-chemicals field, this study has goals the production of albocycline and its analogous from fermentation process. Furthermore, the compounds showed a fitotoxic activity against Lemna minor. A Streptomyces sp. - CAAT 7-52 strain isolated from Caatinga, an unique Brazilian biome, was cultivated in five different types of liquid medium: PD (Potato-Dextrose), PMB (Beef extract, yeast extract, dextrose, tryptose, starch, CaCO3, trace solution), ISP-2 (Yeast extract, malt extract, dextrose), Czapeck (CZ) (Sucrose, K2HPO4, MgSO4, FeSO4, NaNO3) and a modified Czapeck (CZ-GL) with sucrose replaced by glucose syrup for 7 days at 130 rpm and 30° C. The secondary metabolites were extracted with ethyl acetate and characterized LC-MS/MS system (Acquity Xevo TQ-S, Waters Corp.). Activity against Lemna minor was obtained by bioassay in SIS (Swedish Standard) medium for 7 days incubated in photoperiod chamber at 28° C, using Atrazine as positive control and DMSO e SIS medium as negative controls. Bioautography assay used TLC with ethyl acetate / hexane (1:1) as mobile phase, covered with agar and Lemna minor placed in the fractions zones. Atrazine was the positive control and agar the negative control. The chromatogram (Fig. B) and mass spectra analyses showed a pattern of peaks assigned to albocycline (m/z 639 [2M+Na+]+, m/z 331 [M+Na+]+ and m/z 309 [M+H+]+) also its analogous forms all isomers with the molecular mass 324.2 Da (m/z 347 [M+Na+]+ and m/z 671 [2M+Na+]+) which also revealed the same activity as albocycline. Accordant to the chromatogram and mass spectra analyses, albocycline was present in the extracts come from PD, PMB and CZ-GL. The analogous was detected only in the PD and ISP-2 extract and the CZ extract didn?t produce any compounds. The extracts were them summited to bioassay against the aquatic plant Lemna minor and all the extracts (with exception for CZ) exhibited a strong fitotoxic activity by the necrosis of the plant leaves. Therefore, was observed how the nutrient medium composition changes the secondary metabolites biosynthesis and how it can affect the extract bioactivity, as well a strong fitotoxic activity with no registers in literature.
Thesagro: | Caatinga.
Thesaurus Nal: | Streptomyces.
Categoria do assunto: | V Taxonomia de Organismos
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16032-1) - UPC - SP - DD -  -  - itamar - DIS