Data/hora: 16/06/2024 06:45:33

Biblioteca(s): | Embrapa Agroenergia; Embrapa Amazônia Oriental; Embrapa Pantanal; Embrapa Suínos e Aves.
Data corrente: | 09/05/2016
Data da última atualização: | 10/05/2018
Autoria: | HADI, S. I. I. A; SANTANA, H.; BRUNALE, P. P. M.; GOMES, T. G.; OLIVEIRA, M. D. de; MATTHIENSEN, A.; OLIVEIRA, M. E. C.; SILVA, F. C. P.; BRASIL, B. dos S. A. F.
Afiliação: | SÁMED, I. I. A. HADI, UFT; HUGO SANTANA, UFBA; PATRÍCIA P. M. BRUNALE, UnB; TAÍSA G. GOMES, UFT; MARCIA DIVINA DE OLIVEIRA, CPAP; ALEXANDRE MATTHIENSEN, CNPSA; MARCOS ENE CHAVES OLIVEIRA, CPATU; FLÁVIA C. P. SILVA; BRUNO DOS SANTOS ALVES FIGUEIREDO B, CNPAE.
Título: | DNA barcoding green microalgae isolated from neotropical inland waters.
Ano de publicação: | 2016
Fonte/Imprenta: | Plos One, v. 11, n. 2, Feb. 2016.
DOI: | http://dx.doi.org/10.1371/journal.pone.0149284
Idioma: | Inglês
Conteúdo: | This study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences? using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker.
Palavras-Chave: | Análise molecular; Identificação morfológica; Microalgas; Sequenciamento genético.
Thesagro: | Alga.
Thesaurus Nal: | Algae; Microalgae.

Categoria do assunto:

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P Recursos Naturais, Ciências Ambientais e da Terra
V Taxonomia de Organismos

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/176636/1/journal.pone.0149284.PDF
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/152647/1/journal.pone.0149284.PDF

Registro original: | Embrapa Suínos e Aves (CNPSA)

Exemplares:

CNPAE - (2883-1) - UPC - AP - DD -  -  -  - DIS
CNPSA - (20666-1) - UPC - AP - DD -  -  -  - DIS
CPAP - (59721-1) - UPC - AP - PP - SP18215 -  - 18215 - DIS
CPATU - (53135-1) - UPC - AP - DD -  -  -  - DIS