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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
06/01/2009 |
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Data da última atualização: |
17/04/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; OLIVEIRA, M. C. de S.; ROCHA, R. B.; SILVA NETTO, F. G.; CAVALCANTE, F. A. |
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Afiliação: |
Luciana Gatto Brito, Embrapa Rondônia; Márcia Cristina De Sena Oliveira, Embrapa Pecuária Sudeste; Rodrigo Barros Rocha, Embrapa Rondônia; Francelino Goulart da Silva Netto, Embrapa Rondônia; Francisco Aloísio Cavalcante, Embrapa Acre. |
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Título: |
Estudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. |
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Idioma: |
Português |
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Notas: |
Meta 2008. |
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Conteúdo: |
Bovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided MenosBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of ... Mostrar Tudo |
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Palavras-Chave: |
Anaplasma marginele; Estudo genético advergência. |
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Thesagro: |
Gene; Proteína. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02650naa a2200229 a 4500
001 1709264
005 2009-04-17
008 2008 bl --- 0-- u #d
100 1 $aBRITO, L. G.
245 $aEstudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1a.
260 $c2008
500 $aMeta 2008.
520 $aBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 µl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided
650 $aGene
650 $aProteína
653 $aAnaplasma marginele
653 $aEstudo genético advergência
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aROCHA, R. B.
700 1 $aSILVA NETTO, F. G.
700 1 $aCAVALCANTE, F. A.
773 $tIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
21/09/2017 |
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Data da última atualização: |
04/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PRESTES, D. C. V.; TONATO, F.; SOUZA, J. N.; PERIN, R. |
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Afiliação: |
Daniel Cid Vieira Prestes, Bolsista de Iniciação Científica, Paic/Fapeam/CPAA; FELIPE TONATO, CPPSE; JASIEL NUNES SOUSA, CPAA; ROGERIO PERIN, CPAA. |
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Título: |
Avaliação da recuperação da fertilidade do solo de pastagens degradadas: estratégias de reposição da fertilidade. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTIFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 13., 2016, Manaus. Anais... Brasília, DF: Embrapa, 2017. |
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Páginas: |
p. 61-62. |
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Idioma: |
Português |
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Conteúdo: |
Este estudo objetivou avaliar os efeitos do aumento da disponibilidade de nutrientes no solo em função de estratégias de reposição da fertilidade na produtividade de pastagens em degradação na Amazônia Ocidental. |
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Thesagro: |
Adubação; Brachiaria Humidicola; Calagem. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163656/1/XIII-Jornada-IC-61.pdf
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Marc: |
LEADER 00915naa a2200205 a 4500
001 2076063
005 2018-01-04
008 2017 bl --- 0-- u #d
100 1 $aPRESTES, D. C. V.
245 $aAvaliação da recuperação da fertilidade do solo de pastagens degradadas$bestratégias de reposição da fertilidade.$h[electronic resource]
260 $c2017
300 $ap. 61-62.
520 $aEste estudo objetivou avaliar os efeitos do aumento da disponibilidade de nutrientes no solo em função de estratégias de reposição da fertilidade na produtividade de pastagens em degradação na Amazônia Ocidental.
650 $aAdubação
650 $aBrachiaria Humidicola
650 $aCalagem
700 1 $aTONATO, F.
700 1 $aSOUZA, J. N.
700 1 $aPERIN, R.
773 $tIn: JORNADA DE INICIAÇÃO CIENTIFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 13., 2016, Manaus. Anais... Brasília, DF: Embrapa, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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