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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/11/2018 |
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Data da última atualização: |
30/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; GIGLIOTI, R.; Silva, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Rodrigo Giglioti, UNESP; Pamella Cristini Silva, UNICEP; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 2671-2680, 2018. |
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DOI: |
10.1007/s11033-018-4436-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens. MenosBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydro... Mostrar Tudo |
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Palavras-Chave: |
DNA extraction; Hydrolysis probe; Matrix sample; QPCR. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02517naa a2200253 a 4500
001 2100307
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4436-9$2DOI
100 1 $aOKINO, C. H.
245 $aComparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring.$h[electronic resource]
260 $c2018
520 $aBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens.
650 $aBabesiosis
650 $aBabesia Bovis
653 $aDNA extraction
653 $aHydrolysis probe
653 $aMatrix sample
653 $aQPCR
700 1 $aGIGLIOTI, R.
700 1 $aSilva, P. C.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 2671-2680, 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 419 | |
| 64. |  | BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. PASA assay for diagnosing insecticide resistance in the horn fly population in Rondônia. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., 2012, Brasília, DF. Proceedings... Brasília, DF: Embrapa Estudos e Capacitação, 2012. p. 127.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 67. | | BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. PASA assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondônia. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., 2012, Brasília, DF. Proceedings... Brasília, DF: Embrapa Estudos e Capacitação, 2012. p. 125.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 72. | | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; SARRIA, A.; BELLETE, B.; CAZAL, C.; BATALHÃO, J.; FERNANDES, J. Atividade in vitro de extratos vegetais e isolados sobre larvas e fêmeas ingurgitadas de Rhipicephalus ( Boophilus) microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 73. | | KATIKI, L. M.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; VERÍSSIMO, C. J.; FERRENZINI, J. Atividade dos óleos essenciais de mentha piperita, cymbopogon scoenanthus, cymbopogon martinii e rosmarinus officinalis sobre larvas de rhipicephalus (boophilus) microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 74. | | OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S.; FORIM, M.; SILVA, M. F.; GIGLIOTI, R.; CARVALHO, C. O. Ação de extrato de Neem sobre Rhipicephalus ( Boophilus micropulus) com azadirachtina - A quantificada por HPLC. In: REUNION ASOCIACION LATINOAMERICANA DE PRODUCCION ANIMAL, 20.; REUNION ASOCIACION PERUANA DE PRODUCCION ANINAL, 30.; CONGRESSO INTERNACIONAL DE GANDEIRA DE DOBLE PROPOSTO, 5., 2007, Cuzco, Perú. Annales... Cuzco: ALPA: APPA, 2007. 1 CD-ROM. Resumo na seguinte fonte: Archivos Latinoamericano de Produccion Animal, v. 15, (supl.), p. 489, 2007| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 77. | | PORTILHO, A. I.; GIGLIOTI, R.; OLIVEIRA, H. N. de; MARCONDES, C. R.; OKINO, C. H.; OLIVEIRA, M. C. de S. Associação entre os níveis de infecção por Babesia bovis e Babesia bigemina em amostras de sangue e carrapatos Rhipicephalus microplus colhidos em bovinos da raça Canchim. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 37. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 79. | | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; FORIM, M. R.; GIGLIOTI, R.; MIGLIATO, M. A. T. Avaliação do óleo de Neem com teor padronizado de azadirachtina - A sobre as larvas de Rhipicephalus Boophilus microplus. In: CONGRESSO BRASILEIRA DE VETERINÁRIA, 34., 2007, Santos. Resumos... São Paulo, Sociedade Paulista de Medicina Veterinária, 2007. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 80. | | FUNES-HUACCA, M.E.; GIGLIOTI, R.; NÉO, T. A.; CARRILHO, E.; BRITO, L. G.; OLIVEIRA, M. C. de S. ANÁLISE DO PERFIL PROTÉICO DOS PRODUTOS DE EXCREÇÃO/SECREÇÃO DE LARVAS DE Cochliomyia hominivorax. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUDESTE, 2006, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 419 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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