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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/01/2011 |
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Data da última atualização: |
10/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
STOLF-MOREIRA, R.; LEMOS, E. G. M.; CARARETO-ALVES, L.; MARCONDES, J.; PEREIRA, S. S.; ROLLA, A. A. P.; PEREIRA, R. M.; NEUMAIER, N.; BINNECK, E.; ABDELNOOR, R. V.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; FARIAS, J. R. B.; NEPOMUCENO, A. L. |
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Afiliação: |
RENATA STOLF-MOREIRA, UNESP Jaboticabal; ELIANA G. M. LEMOS, UNESP Jaboticabal; LÚCIA CARARETO-ALVES, UNESP Jaboticabal; JACKSON MARCONDES, UNESP Jaboticabal; SELMA S. PEREIRA, UEL; AMANDA A. P. ROLLA, UEL; RODRIGO M. PEREIRA, UNESP JABOTICABAL; NORMAN NEUMAIER, CNPSO; ELISEU BINNECK, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptional profiles of roots of different soybean genotypes subjected to drought stress. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 29, n. 1, p. 19-34, 2011. |
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DOI: |
10.1007/s11105-010-0203-3 |
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Idioma: |
Inglês |
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Conteúdo: |
To identify differentially expressed genes in soybean grown under different drought conditions, cDNA libraries from roots of different genotypes were constructed. Genes of contrasting genotypes of soybean were found to be differentially expressed in plants exposed to drought conditions. A total of 753 no redundant clones were identified by PCR, and these were printed on microarray glass slides. Probes of total RNA were prepared from bulked roots subjected to 25 and 50 min (Bulk 1) or 75 and 100 min (Bulk 2) of drought stress. Differential expression of 145 genes, involved in metabolic pathways responsive to biotic and abiotic stresses, was observed. These genes were classified into nine functional categories, including energy, transcription factors, metabolism, stress response, protein synthesis, cell communication, cell cycle, cell transport, and unknown function. The functionality of some of these genes was confirmed by quantitative real-time PCR (qRT-PCR). |
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Thesagro: |
Deficiencia hidrica; Genotipo; Soja. |
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Thesaurus Nal: |
Genotype; Soybeans; Water stress. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01991naa a2200361 a 4500
001 1872417
005 2017-05-10
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s11105-010-0203-3$2DOI
100 1 $aSTOLF-MOREIRA, R.
245 $aTranscriptional profiles of roots of different soybean genotypes subjected to drought stress.
260 $c2011
520 $aTo identify differentially expressed genes in soybean grown under different drought conditions, cDNA libraries from roots of different genotypes were constructed. Genes of contrasting genotypes of soybean were found to be differentially expressed in plants exposed to drought conditions. A total of 753 no redundant clones were identified by PCR, and these were printed on microarray glass slides. Probes of total RNA were prepared from bulked roots subjected to 25 and 50 min (Bulk 1) or 75 and 100 min (Bulk 2) of drought stress. Differential expression of 145 genes, involved in metabolic pathways responsive to biotic and abiotic stresses, was observed. These genes were classified into nine functional categories, including energy, transcription factors, metabolism, stress response, protein synthesis, cell communication, cell cycle, cell transport, and unknown function. The functionality of some of these genes was confirmed by quantitative real-time PCR (qRT-PCR).
650 $aGenotype
650 $aSoybeans
650 $aWater stress
650 $aDeficiencia hidrica
650 $aGenotipo
650 $aSoja
700 1 $aLEMOS, E. G. M.
700 1 $aCARARETO-ALVES, L.
700 1 $aMARCONDES, J.
700 1 $aPEREIRA, S. S.
700 1 $aROLLA, A. A. P.
700 1 $aPEREIRA, R. M.
700 1 $aNEUMAIER, N.
700 1 $aBINNECK, E.
700 1 $aABDELNOOR, R. V.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aMARCELINO, F. C.
700 1 $aFARIAS, J. R. B.
700 1 $aNEPOMUCENO, A. L.
773 $tPlant Molecular Biology Reporter, Athens$gv. 29, n. 1, p. 19-34, 2011.
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Registro original: |
Embrapa Soja (CNPSO) |
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