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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/06/2013 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTRO, R. DE J. C.; SOUZA SOBRINHO, F. de; GAMA, M. A. S. da; BARRA, P. M. DE C.; ANTONIASSI, R.; OLIVEIRA, M. A. L. DE. |
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Afiliação: |
RENATA DE JESUS COELHO CASTRO, UFJF; FAUSTO DE SOUZA SOBRINHO, CNPGL; MARCO ANTONIO SUNDFELD DA GAMA, CNPGL; PATRICIA M. DE CASTRO BARRA, UFJF; ROSEMAR ANTONIASSI, CTAA; MARCONEI A. L. DE OLIVEIRA, UFJF. |
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Título: |
Analysis of the main conjugated linoleic acid (CLA) precursors (C18:2 n-6 and C18:3 n-3) in Brachiaria ruzizienses by capillary zone electrophoresis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Central European Journal of Chemistry, v. 11, n. 8, p. 1286-1296, 2013. |
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Páginas: |
p. 1286-1296 |
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Idioma: |
Inglês |
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Conteúdo: |
An alternative method for extraction optimization of C18:2 n-6 and C18:3 n-3, the main precursors for the synthesis of conjugated linoleic acid (CLA), in Brachiaria ruzizienses forages was proposed. Three methods of lipid extraction were tested: 1. Hara & Radin, 2. Micro Folch and 3. Bligh & Dyer. The preliminary test showed the Hara & Radin method as the most promising procedure. Then, a 33Box Behnken design with triplicate in the central point was applied in Hara & Radin method in order to optimize the extraction procedure. The optimization extraction was monitored by quantification of C18:2 n-6 and C18:3 n-3 through capillary zone electrophoresis (CZE). The results obtained by CZE were compared to gas chromatography (AOCS official method) in real samples using the paired t-test. No significant difference between methods was found within a 95% confidence interval (p-value= 0.937). The alternative CZE method for Brachiaria ruzizienses forages analysis has some advantages in comparison with official GC method such as, short analysis time (10 min), no derivatization step for sample preparation, absence of specific separation columns, lower analytical cost and high throughput. |
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Palavras-Chave: |
Desenho fatorial; Metodo de extração; Precurssor CLA. |
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Thesagro: |
Forragem. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97936/1/Artigo-Fausto-Analysis-art3A10.24782Fs11532-013-0262-z.pdf
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Marc: |
LEADER 01998naa a2200241 a 4500
001 1959987
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aCASTRO, R. DE J. C.
245 $aAnalysis of the main conjugated linoleic acid (CLA) precursors (C18$b2 n-6 and C18:3 n-3) in Brachiaria ruzizienses by capillary zone electrophoresis.$h[electronic resource]
260 $c2013
300 $ap. 1286-1296
520 $aAn alternative method for extraction optimization of C18:2 n-6 and C18:3 n-3, the main precursors for the synthesis of conjugated linoleic acid (CLA), in Brachiaria ruzizienses forages was proposed. Three methods of lipid extraction were tested: 1. Hara & Radin, 2. Micro Folch and 3. Bligh & Dyer. The preliminary test showed the Hara & Radin method as the most promising procedure. Then, a 33Box Behnken design with triplicate in the central point was applied in Hara & Radin method in order to optimize the extraction procedure. The optimization extraction was monitored by quantification of C18:2 n-6 and C18:3 n-3 through capillary zone electrophoresis (CZE). The results obtained by CZE were compared to gas chromatography (AOCS official method) in real samples using the paired t-test. No significant difference between methods was found within a 95% confidence interval (p-value= 0.937). The alternative CZE method for Brachiaria ruzizienses forages analysis has some advantages in comparison with official GC method such as, short analysis time (10 min), no derivatization step for sample preparation, absence of specific separation columns, lower analytical cost and high throughput.
650 $aForragem
653 $aDesenho fatorial
653 $aMetodo de extração
653 $aPrecurssor CLA
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aGAMA, M. A. S. da
700 1 $aBARRA, P. M. DE C.
700 1 $aANTONIASSI, R.
700 1 $aOLIVEIRA, M. A. L. DE
773 $tCentral European Journal of Chemistry$gv. 11, n. 8, p. 1286-1296, 2013.
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Embrapa Gado de Leite (CNPGL) |
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