|
|
Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
16/03/2023 |
Data da última atualização: |
16/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, L. C. de; NAKASONE, A. K.; SILVA, C. S.; BONFIM, K. |
Afiliação: |
LUANA CARDOSO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO PARÁ; ALESSANDRA KEIKO NAKASONE, CPATU; CAIO SANTOS SILVA, UNIVERSIDADE FEDERAL DO PARÁ; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen. |
Título: |
Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Revista Ceres, Viçosa, v. 70, n. 1, p. 124-132, jan./feb. 2023. |
DOI: |
https://doi.org/10.1590/0034-737X202370010014 |
Idioma: |
Inglês |
Conteúdo: |
Bacterial spot, caused by Xanthomonas axonopodis pv. passiflorae, is a disease that has limited the cultivation of passionfruit in various orchards in Brazil. The objective of this work is to characterize and evaluate the variability of 29 strains of X. axonopodis pv. passiflorae from different municipalities producing yellow passionfruit in the state of Pará. The characterization was performed by the biochemical methods of KOH solubility, oxidase and Bactray, and the molecular methods of Xapas-F, Xapas-Ri and Xapas-Ro primers. The variability was evaluated by pathogenicity test and RAPD (randomly amplified polymorphic DNA). The strains of X. axonopodis pv. passiflorae were Gram-negative and oxidase-negative, and tests with the Bactray kit showed no relation between the collection municipality and group composition. The Xapas-F/Ri/Ro primers were specific for the strains. The primers used amplified 118 polymorphic bands in the RAPD reactions and the highest genetic similarity was between the strains PA15 and PA16. As the pathogenicity test evidenced a pathogenic variability, the strains PA2.1, PA4.5, PA14, PA4.2, PA4.1, PA4.6, PA3.4 and PA4.3 present the highest severity values for the disease. The strains of X. axonopodis pv. passiflorae show characteristics typical of the species, and genetic and pathogenic variability among them. |
Palavras-Chave: |
Bacterial spot; Biochemical tests. |
Thesagro: |
Doença de Planta; Mancha Bacteriana; Maracujá; Passiflora Edulis Flavicarpa. |
Thesaurus Nal: |
Detection. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152411/1/CharacterizationVariabilityStrainsXanthomonas.pdf
|
Marc: |
LEADER 02174naa a2200253 a 4500 001 2152411 005 2023-03-16 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/0034-737X202370010014$2DOI 100 1 $aOLIVEIRA, L. C. de 245 $aCharacterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.$h[electronic resource] 260 $c2023 520 $aBacterial spot, caused by Xanthomonas axonopodis pv. passiflorae, is a disease that has limited the cultivation of passionfruit in various orchards in Brazil. The objective of this work is to characterize and evaluate the variability of 29 strains of X. axonopodis pv. passiflorae from different municipalities producing yellow passionfruit in the state of Pará. The characterization was performed by the biochemical methods of KOH solubility, oxidase and Bactray, and the molecular methods of Xapas-F, Xapas-Ri and Xapas-Ro primers. The variability was evaluated by pathogenicity test and RAPD (randomly amplified polymorphic DNA). The strains of X. axonopodis pv. passiflorae were Gram-negative and oxidase-negative, and tests with the Bactray kit showed no relation between the collection municipality and group composition. The Xapas-F/Ri/Ro primers were specific for the strains. The primers used amplified 118 polymorphic bands in the RAPD reactions and the highest genetic similarity was between the strains PA15 and PA16. As the pathogenicity test evidenced a pathogenic variability, the strains PA2.1, PA4.5, PA14, PA4.2, PA4.1, PA4.6, PA3.4 and PA4.3 present the highest severity values for the disease. The strains of X. axonopodis pv. passiflorae show characteristics typical of the species, and genetic and pathogenic variability among them. 650 $aDetection 650 $aDoença de Planta 650 $aMancha Bacteriana 650 $aMaracujá 650 $aPassiflora Edulis Flavicarpa 653 $aBacterial spot 653 $aBiochemical tests 700 1 $aNAKASONE, A. K. 700 1 $aSILVA, C. S. 700 1 $aBONFIM, K. 773 $tRevista Ceres, Viçosa$gv. 70, n. 1, p. 124-132, jan./feb. 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 362 | |
9. | | DIÓGENES, M. B.; CUNHA, R. M.; OLIVEIRA, L. C. de. Caracterização florística, fisionômica e estrutural da vegetação lenhosa da bacia do Rio Xapuri, com ênfase na seringueira e com uso de ferramentas de precisão, Acre, Brasil. In: CONGRESSO DE ECOLOGIA DO BRASIL, 10.; SIMPÓSIO DE SUSTENTABILIDADE, 1., 2011, São Lourenço. Sustentabilidade. [S.l.]: Sociedade de Ecologia do Brasil, 2011. 2 p. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Acre. |
| |
16. | | SUTIL, W. P.; SANTOS, R. S.; OLIVEIRA, L. C. de. Controle químico de cigarrinhas-das-pastagens no estado do Acre. In: SEMINÁRIO DA EMBRAPA ACRE DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO, 2., 2019, Rio Branco, AC. A Contribuição da ciência para a agropecuária no Acre: anais. Rio Branco, AC: Embrapa Acre, 2020. p. 101-106. Banner. (Embrapa Acre. Eventos técnicos & científicos, 2). Editores técnicos: Virgínia de Souza Álvares; Fabiano Marçal Estanislau.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Acre. |
| |
Registros recuperados : 362 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|