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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/11/2016 |
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Data da última atualização: |
07/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIGLIOTI, R.; OLIVEIRA, H. N. de; SANTANA, C. H.; IBELLI, A. M. G.; NEO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. |
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Afiliação: |
Rodrigo Giglioti, UNESP; Henrique Nunes de Oliveira, UNESP; Clarissa Helena Santana, UNESP; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Thalita Athiê Néo, UFSCAR; Talita Barban Bilhassi, UNESP; MARCIO DIAS RABELO, CPPSE; Rosângela Zacarias Machado, UNESP; LUCIANA GATTO BRITO, CPAF-Rondonia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 7, n. 5, p. 657-662, jul. 2016. |
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ISSN: |
1877-959X |
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Idioma: |
Inglês |
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Conteúdo: |
The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. MenosThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus a... Mostrar Tudo |
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Palavras-Chave: |
QPCR; R microplus; Resistance. |
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Thesagro: |
Babesia Bovis; Bovino. |
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Thesaurus Nal: |
babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03252naa a2200313 a 4500
001 2057560
005 2018-11-07
008 2016 bl uuuu u00u1 u #d
022 $a1877-959X
100 1 $aGIGLIOTI, R.
245 $aBabesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil.$h[electronic resource]
260 $c2016
520 $aThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal.
650 $ababesiosis
650 $aBabesia Bovis
650 $aBovino
653 $aQPCR
653 $aR microplus
653 $aResistance
700 1 $aOLIVEIRA, H. N. de
700 1 $aSANTANA, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aNEO, T. A.
700 1 $aBILHASSI, T. B.
700 1 $aRABELO, M. D.
700 1 $aMACHADO, R. Z.
700 1 $aBRITO, L. G.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 7, n. 5, p. 657-662, jul. 2016.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 212 | |
| 201. |  | SILVEIRA, L. G. G.; FURLAN, L. R.; CURI, R. A.; FERRAZ, A. L. J.; ALENCAR, M. M. de; REGITANO, L. C. de A.; MARTINS, C. L.; ARRIGONI, M. de B.; SUGUISAWA, L.; SILVEIRA, A. C.; OLIVEIRA, H. N. de. Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model. Genetics and Molecular Biology, v. 31, n. 4, p. 874-879, 2008| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 202. | | PEREIRA, F. F.; CARDOSO, C. R. G.; LUCCHETTA, J. T.; OLIVEIRA, H. N. de; SANTOS, J. P.; RAMOS, L. N.; ALENCAR, M. X.; VEIGA, V. C.; LEITE, M. K.; MENDES, J. P. Protocolo de produção de eulofídeos para controle biológico de lepidópteros desfolhadores em plantios de eucalipto. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 16., 2019, Londrina. Controle biológico: da academia ao campo, rumo à sustentabilidade: anais. Londrina: Sociedade Entomológica do Brasil, 2019. p. 228| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 203. | | BORGES, B. O.; CURI, R. A.; BALDI, F.; FEITOSA, F. L. B.; ANDRADE, W. B. F. de; ALBUQUERQUE, L. G. de; OLIVEIRA, H. N. de; CHARDULO, L. A. L. Polymorphisms in candidate genes and their association with carcass traits and meat quality in Nellore cattle. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 5, p. 364-371, maio. 2014. Título em português: Polimorfismos em genes-candidatos e suas associações com características de carcaça e qualidade da carne em bovinos Nelore.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 204. | | NEO, T. A.; GIGLIOTI, R.; OBREGON, D.; BILHASSI, T. B.; OLIVEIRA, H. N. de; MACHADO, R. Z.; ANIBAL, F. de F.; BRITO, L. G.; MALAGO JUNIOR, W.; DONATONI, F. A. B.; OLIVEIRA, M. C. de S. Detection of Babesia bovis and Babesia bigemina in water buffaloes (Bubalus bubalis) in endemic areas of São Paulo state, Brazil. Open Journal of Veterinary Medicine, v. 6, n. 5, p. 75-84, maio 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 205. | | CAMPOS, G. S.; CARDOSO, F. F.; GULIAS GOMES, C. C.; DOMINGUES, R.; REGITANO, L. C. de A.; OLIVEIRA, M. C. de S.; OLIVEIRA, H. N. DE; CARVALHEIRO, R.; ALBUQUERQUE, L. G.; MILLER, S.; MISTZAL, I.; LOURENCO, D. Development of genomic predictions for Angus cattle in Brazil incorporating genotypes from related american sires. Journal of Animal Science, v. 100, n. 2, p. 1-13, Feb. 2022. skac009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 206. | | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; ESTEVES, S. N.; OLIVEIRA, H. N. DE; GIGLIOTI, R.; GIGLIOTI, C.; CARVALHO, C. DE O.; FERREZINI, J.; SCHIAVONE, D. C. Parasitismo por nematóides gastrintestinais em matrizes e cordeiros criados em São Carlos, São Paulo. Revista Brasileira de Parasitologia Veterinária, v. 17, Supl. 1, p. 126-132, 2008.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 207. | | SILVA, P. C.; MARTINS, M. D. R.; TONELLI, M. F.; GOMES, H. C. S. F.; OLIVEIRA, H. N. DE; VELTRONI, Y. K.; SIMAS, P. V.; BASSETTO, C. C.; GIGLIOTI, R.; OLIVEIRA, M. C. de S.; OKINO, C. H. Perfis de anticorpos sistêmicos dos isótipos IgG e IgM anti-Babesia bovis e anti-Babesia bigemina em bezerros da raça Canchim do nascimento até 12 meses de idade. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 12., 2020, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2020. p.28. (Embrapa Instrumentação. Documentos, 71).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 208. | | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; WEDY, B. C. R.; GUTMANIS, G.; VERÍSSIMO, C. J.; KATIKI, L. M.; VERCESI FILHO, A. E.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis. Ticks and Tick-borne Diseases, v.12, n.5, 101753, sep. 2021. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 209. | | AZEVEDO, B. T.; OLIVEIRA, H. N. DE; KATIKI, L. M.; VERCESI FILHO, A. E.; DOMINGOS, A. G.; ANTUNES, S.; OKINO, C. H.; OLIVEIRA, M. C. de S.; IBELLI, A. M. G.; GIGLIOTI, R. A small proportion of Zebu genetic background in crossbred calves may not be enough to improve resistance against natural bovine Babesia spp. infections. Veterinary Parasitology, v. 328, 2024, 110165. 7 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 210. | | GIGLIOTI, R.; OLIVEIRA, H. N. de; GUTMANIS, G.; LUCIANI, G. F.; AZEVEDO, B. T.; FIORIN, C. F. de C.; ANDRADE, M. F. de; SILVA, M. A. F.; VERCESI FILHO, A. E.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; VERÍSSIMO, C. J. Correlations and repeatability between Babesia spp. infection levels using two dairy cattle breeding systems. Experimental and Applied Acarology, v. 81, p. 599-607, jul. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 211. | | ROMERO, A. R. da S.; NASCIMENTO, A. V. do; OLIVEIRA, M. C. de S.; OKINO, C. H.; BRAZ, C. U.; SCALEZ, D. C. B.; CARDOSO, D. F.; CARDOSO, F. F.; GULIAS GOMES, C. C.; CAETANO, A. R.; TONHATI, H.; GONDRO, C.; OLIVEIRA, H. N. de. Genetic parameters and multi-trait genomic prediction for hemoparasites infection levels in cattle. Livestock Science, v. 273, 2023. 105259. 7 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia. |
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| 212. | | CARDOSO, D. F.; FERNANDES JÚNIOR, G. A.; SCALEZ, D. C. B.; ALVEZ, A. A. C.; MAGALHÃES, A. F. B.; BRESOLIN, T.; VENTURA, R. V.; LI, C.; OLIVEIRA, M. C. de S.; PORTO NETO, L. R.; CARVALHEIRO, R.; OLIVEIRA, H. N. de; TONHATI, H.; ALBUQUERQUE, L. G. Uncovering sub-structure and genomic profiles in across-countries subpopulations of Angus Cattle. Scientific Reports, v. 10, article 8770, 2020. 11 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 212 | |
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