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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de. |
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Afiliação: |
G. A. F. OLIVEIRA, UFRB; JORGE LUIZ LOYOLA DANTAS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development and validation of minisatellite markers for Carica papaya. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Biologia Plantarum, v. 59, n.4, p 686-694, 2015. |
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DOI: |
10.1007/s10535-015-0551-9686 |
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Idioma: |
Inglês |
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Conteúdo: |
Whereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding. MenosWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. ... Mostrar Tudo |
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Thesagro: |
Carica Papaya; Mamão; Variação genética. |
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Thesaurus Nal: |
Genetic variation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02343naa a2200205 a 4500
001 2028333
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s10535-015-0551-9686$2DOI
100 1 $aOLIVEIRA, G. A. F.
245 $aDevelopment and validation of minisatellite markers for Carica papaya.$h[electronic resource]
260 $c2015
520 $aWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding.
650 $aGenetic variation
650 $aCarica Papaya
650 $aMamão
650 $aVariação genética
700 1 $aDANTAS, J. L. L.
700 1 $aOLIVEIRA, E. J. de
773 $tBiologia Plantarum$gv. 59, n.4, p 686-694, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 5. |  | SILVA, I. C. da; OLIVEIRA, M. do S. P. de; FORTES, A. C. R. Seleção de primers ISSR para análises genéticas em Tucumã-do-Pará (Astrocaryum vulgare Mart.). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | SOUSA, A. C. G. de; MOURA, E. F.; SOUZA, L. S.; SILVA, I. C. da; LEITE, I. N. Otimização do protocolo de extração de DNA de mandioca pela adição de diferentes antioxidantes. In: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Unir talentos para ultrapassar fronteiras. Fortaleza: Embrapa Agroindústria Tropical, 2014. p. 23. 19º MET.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | FORTES, A. C. R.; OLIVEIRA, M. do S. P. de; OLIVEIRA, N. P. de; SILVA, I. C. da. Transferibilidade de locos SSR de Astrocaryum aculeatum Mart. para Astrocaryum murumuru Mart. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | SILVA, I. C. da; FEITOSA, K. A.; FOGLIO, M. A.; MAGALHÃES, P. M.; SOARES, V. E.; OLIVEIRA, G. P. de; CHAGAS, A. C. de S. Avaliação antiparasitária de Artemisia annua L. (Asteraceae) sobre o carrapato Rhipicephalus (Boophilus) microplus (Canestri, 1887) (ACARI: IXODEDAE) em bovinos sob infestação natural. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. p. 160. Resumo PA30.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | SOUZA, L. S.; OLIVEIRA, M. do S. P. de; SOUSA, A. C. G. de; SILVA, I. C. da; LEITE, I. N. Aprimoramento de protocolo de extração de DNA para duas espécies de palmeiras com o uso de diferentes antioxidantes. In: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Unir talentos para ultrapassar fronteiras. Fortaleza: Embrapa Agroindústria Tropical, 2014. p. 24. 19º MET.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SILVA, I. C. da; MAGALHAES, P. M. de; SOUSA, I. M. de O.; FOGLIO, M. A.; LEONARDECZ, E.; ESTEVES, S. N.; CHAGAS, A. C. de S. Anthelmintic activity of Artemisia annua in sheepmodel. Journal of Medicinal Plants Research, v. 11, n. 7, p. 137-143, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | ROSA, C. E.; FURLANI JÚNIOR, E.; FERRARI, S.; LUQUES, A. P. P. G.; FERRARI, J. V.; SANTOS, D. M. A. dos; SILVA, I. C. da; ROSSETTO, J. E. Aplicação de subdoses de PARAQUAT e características vegetativas e produtivas do algodoeiro. IN: CONGRESSO BRASILEIRO DO ALGODÃO, 8.; COTTON EXPO, 1., 2011, São Paulo. Evolução da cadeia para construção de um setor forte: Anais. Campina Grande, PB: Embrapa Algodão, 2011. p. 612-617| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 36 | |
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