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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/12/2021 |
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Data da última atualização: |
03/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OGBONNA, A. C.; ANDRADE, L. R. B. de; RABBI, I. Y.; MUELLER, L. A.; OLIVEIRA, E. J. de; BAUCHET, G. J. |
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Afiliação: |
ALEX C. OGBONNA, Cornell University; LUCIANO ROGERIO BRAATZ DE ANDRADE; ISMAIL Y. RABBI, International Institute of Tropical Agriculture; LUKAS A. MUELLER, Cornell University; EDER JORGE DE OLIVEIRA, CNPMF; GUILLAUME J. BAUCHET, Boyce Thompson Institute. |
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Título: |
Large-scale genome-wide association study, using historical data, identifies conserved genetic architecture of cyanogenic glucoside content in cassava (Manihot esculenta Crantz) root. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
The Plant Journal, v.105, p.754?770, 2021. |
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ISSN: |
1365-313X |
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DOI: |
10.1111/tpj.15071 |
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Idioma: |
Inglês |
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Conteúdo: |
Manihot esculenta (cassava) is a root crop originating from South America that is a major staple in the tropics, including in marginal environments. This study focused on South American and African germplasm and investigated the genetic architecture of hydrogen cyanide (HCN), a major component of root quality. HCN, representing total cyanogenic glucosides, is a plant defense component against herbivory but is also toxic for human consumption. We genotyped 3354 landraces and modern breeding lines originating from 26 Brazilian states and 1389 individuals were phenotypically characterized across multi-year trials for HCN. All plant material was subjected to high-density genotyping using genotyping by sequencing. We performed genome-wide association mapping to characterize the genetic architecture and gene mapping of HCN. Field experiments revealed strong broad- and narrow-sense trait heritability (0.82 and 0.41, respectively). Two major loci were identified, encoding for an ATPase and a MATE protein, and contributing up to 7 and 30% of the HCN concentration in roots, respectively. We developed diagnostic markers for breeding applications, validated trait architecture consistency in African germplasm and investigated further evidence for the domestication of sweet and bitter cassava. Fine genomic characterization revealed: (i) the major role played by vacuolar transporters in regulating HCN content; (ii) the co-domestication of sweet and bitter cassava major alleles are dependent upon geographical zone; and (iii) the major loci allele for high HCN in M. esculenta Crantz seems to originate from its ancestor, M. esculenta subsp. flabellifolia. Taken together, these findings expand our insights into cyanogenic glucosides in cassava roots and its glycosylated derivatives in plants. MenosManihot esculenta (cassava) is a root crop originating from South America that is a major staple in the tropics, including in marginal environments. This study focused on South American and African germplasm and investigated the genetic architecture of hydrogen cyanide (HCN), a major component of root quality. HCN, representing total cyanogenic glucosides, is a plant defense component against herbivory but is also toxic for human consumption. We genotyped 3354 landraces and modern breeding lines originating from 26 Brazilian states and 1389 individuals were phenotypically characterized across multi-year trials for HCN. All plant material was subjected to high-density genotyping using genotyping by sequencing. We performed genome-wide association mapping to characterize the genetic architecture and gene mapping of HCN. Field experiments revealed strong broad- and narrow-sense trait heritability (0.82 and 0.41, respectively). Two major loci were identified, encoding for an ATPase and a MATE protein, and contributing up to 7 and 30% of the HCN concentration in roots, respectively. We developed diagnostic markers for breeding applications, validated trait architecture consistency in African germplasm and investigated further evidence for the domestication of sweet and bitter cassava. Fine genomic characterization revealed: (i) the major role played by vacuolar transporters in regulating HCN content; (ii) the co-domestication of sweet and bitter cassava major alleles are dependen... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
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Marc: |
LEADER 02530naa a2200217 a 4500
001 2137041
005 2021-12-03
008 2021 bl uuuu u00u1 u #d
022 $a1365-313X
024 7 $a10.1111/tpj.15071$2DOI
100 1 $aOGBONNA, A. C.
245 $aLarge-scale genome-wide association study, using historical data, identifies conserved genetic architecture of cyanogenic glucoside content in cassava (Manihot esculenta Crantz) root.$h[electronic resource]
260 $c2021
520 $aManihot esculenta (cassava) is a root crop originating from South America that is a major staple in the tropics, including in marginal environments. This study focused on South American and African germplasm and investigated the genetic architecture of hydrogen cyanide (HCN), a major component of root quality. HCN, representing total cyanogenic glucosides, is a plant defense component against herbivory but is also toxic for human consumption. We genotyped 3354 landraces and modern breeding lines originating from 26 Brazilian states and 1389 individuals were phenotypically characterized across multi-year trials for HCN. All plant material was subjected to high-density genotyping using genotyping by sequencing. We performed genome-wide association mapping to characterize the genetic architecture and gene mapping of HCN. Field experiments revealed strong broad- and narrow-sense trait heritability (0.82 and 0.41, respectively). Two major loci were identified, encoding for an ATPase and a MATE protein, and contributing up to 7 and 30% of the HCN concentration in roots, respectively. We developed diagnostic markers for breeding applications, validated trait architecture consistency in African germplasm and investigated further evidence for the domestication of sweet and bitter cassava. Fine genomic characterization revealed: (i) the major role played by vacuolar transporters in regulating HCN content; (ii) the co-domestication of sweet and bitter cassava major alleles are dependent upon geographical zone; and (iii) the major loci allele for high HCN in M. esculenta Crantz seems to originate from its ancestor, M. esculenta subsp. flabellifolia. Taken together, these findings expand our insights into cyanogenic glucosides in cassava roots and its glycosylated derivatives in plants.
650 $aMandioca
700 1 $aANDRADE, L. R. B. de
700 1 $aRABBI, I. Y.
700 1 $aMUELLER, L. A.
700 1 $aOLIVEIRA, E. J. de
700 1 $aBAUCHET, G. J.
773 $tThe Plant Journal$gv.105, p.754?770, 2021.
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| 11. |  | RIBEIRO, T. F. L.; SILVA, W. L. da; OLIVEIRA, E. J. de A.; BATISTA, P. J. dos S.; OLIVEIRA, E. J. de; SANTANA, A. E. G.; RIFFEL, A. Characterization of volatile organic compounds (VOCS) from a resistant cultivar of cassava (Manihot esculenta). In: ENCONTRO BRASILEIRO DE ECOLOGIA QUÍMICA, 9., 2015, Belo Horizonte. Resumos... Belo Horizonte: UFMG, 2015. p. 87.| Tipo: Resumo em Anais de Congresso |
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