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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
31/03/2017 |
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Data da última atualização: |
13/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. |
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Afiliação: |
CASSIANA DE OLIVEIRA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; GISELA MANUELA DE FRANÇA BETTENCOURT; ERIKA AMANO, UFPR; LUZIANE FRANCISCON, CNPF; MARGUERITE QUOIRIN, UFPR. |
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Título: |
Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. |
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DOI: |
10.1007/s11676-016-0282-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. MenosGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, a... Mostrar Tudo |
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Palavras-Chave: |
Callogenesis; Eucalyptus urograndis; WPM culture medium. |
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Thesagro: |
Micropropagação; Organogênese; Propagação vegetativa. |
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Thesaurus Nal: |
Callus formation; Micropropagation; Organogenesis; Plant propagation. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02512naa a2200313 a 4500
001 2067974
005 2017-12-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s11676-016-0282-6$2DOI
100 1 $aOLIVEIRA, C. de
245 $aMicropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone.$h[electronic resource]
260 $c2017
520 $aGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %.
650 $aCallus formation
650 $aMicropropagation
650 $aOrganogenesis
650 $aPlant propagation
650 $aMicropropagação
650 $aOrganogênese
650 $aPropagação vegetativa
653 $aCallogenesis
653 $aEucalyptus urograndis
653 $aWPM culture medium
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aBETTENCOURT, G. M. de F.
700 1 $aAMANO, E.
700 1 $aFRANCISCON, L.
700 1 $aQUOIRIN, M.
773 $tJournal of Forestry Research$gv. 28, n. 1, p. 29-39, Jan. 2017.
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Embrapa Florestas (CNPF) |
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| Registros recuperados : 52 | |
| 5. |  | CARVALHO, G. R.; OLIVEIRA, C. de. Panorama da pecuária leiteira no Brasil e no mundo. In: YAMAGUCHI, L. C. T.; MENDES, L. C. R.; LIMA, I. B. de; RODRIGUES, C. do C.; RESENDE, M. L. de. (Ed.). Aspectos sócio-econômicos e ambientais da produção de leite. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 27-37.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | CABALLERO, S. U.; STEFANELLI, D.; OLIVEIRA, C. de; VIDAL, R. Necessidades de Nutrientes e Calagem na Produção de Matéria Seca do Feijoeiro num Latossolo Vermelho-Amarelo da Região de Barretos, São Paulo. Pesquisa Agropecuária Brasileira, Brasília, v.22, n.4, p.387-391, abr. 1987.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | OLIVEIRA, C. de; BETTENCOURT, G.; QUOIRIN, M.; DEGENHARDT-GOLDBACH, J. Influência de combinações de TDZ, BAP e ANA na organogênese indireta de Eucalyptus grandis x E. Urophylla. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 19.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 6., 2013, Recife. Anais dos trabalhos. Recife: UFRPE, 2013. 1 p. CD-ROM. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 14. | | OLIVEIRA, C. de; QUOIRIN, M. G. G.; DEGENHARDT-GOLDBACH, J. Influência do ácido naftaleno acético e do 2,4-diclorofenilacético na regeneração in vitro do clone 3336 de Eucalyptus grandis x E. urophylla. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 18. | | OLIVEIRA, C. de; CARVALHO, G. R.; TRAVASSOS, G. F. Os títulos do agronegócio brasileiro: uma análise comparativa entre a percepção dos especialistas no seu lançamento e a situação atual. In: CONGRESSO SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 48., 2010, Campo Grande, MS. Tecnologias, desenvolvimento e integração social. Campo Grande, MS: Sociedade Brasileira de Economia, Administração e Sociologia Rural, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 52 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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