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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/09/2010 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
N. Z. SARAIVA, UNESP/JABOTICABAL; C. S. OLIVEIRA, UNESP/JABOTICABAL; T. A. D. TETZNER, UNESP/JABOTICABAL; M. M. SOUZA, UNESP/JABOTICABAL; M. R. DE LIMA, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; J. M. GARCIA, UNESP/JABOTICABAL. |
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Título: |
Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. |
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Páginas: |
p. 197 |
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Idioma: |
Português |
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Conteúdo: |
One of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast. MenosOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Cytoplasts. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 03893nam a2200229 a 4500
001 1863081
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aBovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes.$h[electronic resource]
260 $aIn: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1$c2010
300 $ap. 197
520 $aOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast.
650 $aoocytes
653 $aBovine
653 $aCytoplasts
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aSOUZA, M. M.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 183 | |
| 81. |  | OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 82. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 83. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 84. | | OLIVEIRA, C. S.; GOMES, F. dos S.; CONSTANT, L. S.; SILVA, L. F. M. da; GODOY, R. L. de O.; TONON, R. V.; CABRAL, L. M. C. Integrated membrane separation processes aiming to concentrate and purify lycopene from watermelon juice. Innovative Food Science and Emerging Technologies, v. 38, p. 149-154, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 85. | | OLIVEIRA, C. S.; GOMES, F. dos S.; SILVA, L. F. M. da; GODOY, R. L. de O.; TONON, R. V.; CABRAL, L. M. C. Integrated membrane separation processes aiming to concentrate and purify lycopene from watermelon juice. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida. Anais. Gramado: SBCTA Regional, 2016. 6 p. Food: the tree that sustains life. 1069.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 86. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R. de; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; BORGES, C. A. V.; GARCIA, J. M.; CAMARGO, L. S. de A. Kinetics data from bovine sex-specific embryo development from three different bulls. Data in Brief, v. 7, p. 1211-1216, June 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 87. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 88. | | MARTINS, G.; LOUREIRO, A. P.; PINTO, P.; NARDUCHE, L.; HAMOND, C.; PENNA, B.; MEDEIROS, M. A.; OLIVEIRA, C. S.; LILENBAUM, W. Estratégias de controle da leptospirose em bovinos naturalmente infectados pelo serovar Hardjo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... [Natal]: Sociedade Brasileira de Microbiologia, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 89. | | ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. Eficiência reprodutiva de novilhas pré-púberes da raça Gir na produção in vitro de embriões. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 90. | | OLIVEIRA, C. S.; CAMARGO, L. S. de A.; SILVA, M. V. G. B.; SARAIVA, N. Z.; QUINTAO, C. C. R.; MACHADO, M. A. Embryo biopsies for genomic selection in tropical dairy cattle. Animal Reproduction, v. 20, n. 2, e20230064, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 91. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. D.; LIMA, M. R.; NICIURA, S. C. M.; FIGUEIRO, M. R.; VANTINI, R.; GARCIA, J. M. Efeitos do uso de moduladores de AMPC durante a pré-maturação in vitro de oócitos bovinos sobre as junções comunicantes e o potencial de senvolvimento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 92. | | VIEIRA, A. C.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; SILVA, T. A.; VIEIRA, C. D.; CAMARGO, L. S. de A. Effect of epigenetic modulators on development of bovine embryos derived from heatshocked oocytes. Animal Reproduction, v. 15, n. 3, p. 483, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 93. | | CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Revista Brasileira de Zootecnia, v. 43, n. 3, p. 120-126, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 94. | | QUINTAO, C. C. R.; SOUZA, E. D.; OLIVEIRA, C. S.; FREITAS, C. de; OLIVEIRA, J. A. S.; CAMARGO, L. S. de A. Effect of handmade biopsy on bovine embryons at the sixth day post in vitro fertilization Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 95. | | NICOLINO, R. R.; CAPANEMA, R. O.; OLIVEIRA, C. S. F. de; PASTRANA, M. E. O.; LOPES, L. B.; HADDAD, J. P. A. Estimating the abortion risk difference in Neospora caninum seropositive dairy cattle in Brazil Ciência Rural, Santa Maria, v. 45, n. 9, p. 1629-1633, set, 2015| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 96. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 97. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 98. | | GERHARDT, B. T.; SINEDINO, L. D.; CAMARGO, A. J. R.; OLIVEIRA, C. S.; RODRIGUES, A. L. R.; ALVES, P. A. M.; SERAPIÃO, R. V.; NOGUEIRA, L. A. G. Effect of hormonal treatments pre-OPU on oocyte recovery and in vitro embryo production in Girolando cows (Bos taurus x Bos indicus). Animal Reproduction, v.10, n.3, p. 501, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 99. | | NARVÁEZ, H. J.; FONTES, R. S.; CARVALHO, B. C. de; IGUMA, L. T.; SERAPIÃO, R. V.; CAMARGO, A. J. R.; OLIVEIRA, C. S.; SHIMODA, E.; MICAN, G. M. Effect of norgestomet on in vitro embryo production in Bos indicus and Bos taurus cows. Animal Reproduction, v.10, n.3, p. 522, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 100. | | CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; SARAIVA, N. Z.; MAIA, G.; NOGUEIRA, B.; QUINTAO, C. C. R.; OLIVEIRA, C. S. Effect of physiological heat stress on HSP70 and CASPASE 3 levels in bovine oocytes. In: ANNUAL CONFERENCE, 52., 2019, San Jose. Beyond possible: remarkable transformation of reproductive biology: abstracts. San Jose: Society for the Study of Reproduction, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 183 | |
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