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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
21/06/2001 |
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Data da última atualização: |
21/06/2001 |
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Autoria: |
JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. |
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Título: |
Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Sugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody. MenosSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem p... Mostrar Tudo |
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Palavras-Chave: |
Bactéria endofítica; Biological nitrogen fixation; BNF; Cana-de-açúcar; Casa de vegetação; Endophytic bacteria; FBN; Fixação bológica de nitrogênio; Greenhouse; Saccharum spp. |
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Thesaurus Nal: |
Gluconacetobacter diazotrophicus; sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03060naa a2200325 a 4500
001 1598609
005 2001-06-21
008 2001 bl --- 0-- u #d
100 1 $aJAMES, E. K.
245 $aFurther observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions.
260 $c2001
520 $aSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody.
650 $aGluconacetobacter diazotrophicus
650 $asugarcane
653 $aBactéria endofítica
653 $aBiological nitrogen fixation
653 $aBNF
653 $aCana-de-açúcar
653 $aCasa de vegetação
653 $aEndophytic bacteria
653 $aFBN
653 $aFixação bológica de nitrogênio
653 $aGreenhouse
653 $aSaccharum spp
700 1 $aOLIVARES, F. L.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSILVA, L. G. da
700 1 $aREIS, V. M.
773 $tJournal of Experimental Botany, London$gv. 52, n. 357, p. 747-760, apr. 2001.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 49 | |
| 21. |  | CONCEIÇÃO, P. M. da; VIEIRA, H. D.; CANELLAS, L. P.; MARQUES JÚNIOR, R. B.; OLIVARES, F. L. Recobrimento de sementes de milho com ácidos húmicos e bactérias diazotróficas endofíticas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 545-548, abr. 2008 Notas Científicas.
Título em inglês: Corn seed coating with humic acids and endophytic diazotrophic bacteria.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 22. | | REIS, V. M.; OLIVEIRA, A. L. M. de; OLIVARES, F. L.; BALDANI, J. I.; BODDEY, R. M.; URQUIAGA, S. Inoculants for sugar cane: scientific bases for the adoption of the technology for biofuel production. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 15th., INTERNATIONAL CONFERENCE OF THE AFRICAN ASSOCIATION FOR BIOLOGICAL NITROGEN FIXATION, 12th., 2007, Cape Town, South Africa. BNF Applications for poverty alleviation. abstracts... Cape Town: University of Technology & Sister Institutions in Cape Town, 2007. 1 p.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 23. | | MARTINEZ-BALMORI, D.; SPACCINI, R.; AGUIAR, N. O.; NOVOTNY, E. H.; OLIVARES, F. L.; CANELLAS, L. P. Molecular characteristics and bioactivity of humic acids isolated from vermicomposts. In: MEETING OF THE INTERNATIONAL HUMIC SUBSTANCES SOCIETY, 17., 2014, Ioannina. Natural organic matter: structure-dynamics innovative applications: book of abstracts. Ioannina: University of Ioannina, 2014. p. 335-336.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 24. | | MARTINEZ-BALMORI, D.; SPACCINI, R.; AGUIAR, N. O.; NOVOTNY, E. H.; OLIVARES, F. L.; CANELLAS, L. P. Molecular characteristics of humic acids isolated from vermicomposts and their relationship to bioactivity. Journal of Agricultural and Food Chemistry, v. 62, n. 47, p. 11412-11419, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 25. | | CYSNE, A. Q.; LOPES, R.; RAIZER, M. D. M.; TOMAZ, J. S.; LOPES, M. T. G.; OLIVARES, F. L. Preparo rápido de amostras de pólen de açaí-solteiro e tucumã-do-amazonas, sem uso de acetólise, para análise em microscópio eletrônico de varredura. Manaus: Embrapa Amazônia Ocidental, 2021. 10 p. (Embrapa Amazônia Ocidental. Comunicado técnico, 151). ODS| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental. |
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| 28. | | REIS, V. M.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JUNIOR, F. B.; BALDANI, J. I.; DOBEREINER, Johanna. Technical approaches to inoculate micropropagated sugar cane plants with Acetobacter diazotrophicus. Plant and Soil, Dordrecht, v. 206, p. 205-211, 1999.| Biblioteca(s): Embrapa Agrobiologia. |
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| 29. | | REIS, V. M.; PEREIRA, W.; HIPÓLITO, G. de S.; OLIVARES, F. L.; BALDANI, J. I.; BODDEY, R. M.; URQUIAGA, S. A sugarcane bacterial inoculant. In: EUROPEAN NITROGEN FIXATION CONFERENCE, 8th., 2008, Gent, Belgium. Abstracts... Belgium: Universiteit Gent, 2008. p. 157. Resumo PS7-11. Parceria: UFRRJ; UENF.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 30. | | PERIN, L.; SILVA, M. F. da; FERREIRA, J. S.; CANUTO, E. L.; MEDEIROS, A. F. A.; OLIVARES, F. L.; REIS, V. M. Avaliação da capacidade de estabelecimento endofítico de estirpes de Azospirillum e Herbaspirillum em milho e arroz. Agronomia, Seropédica/RJ, v. 37, n. 2, p. 47-53, ago./dez. 2003. Evaluation of the endophytic establishment capacity of strain of Azospirillum and Herbaspirillum bacteria in corn and rice.| Biblioteca(s): Embrapa Agrobiologia. |
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| 31. | | CANELLAS, L. P.; DOBBSS, L. B.; OLIVEIRA, A. L.; CHAGAS, J. G.; AGUIAR, N. O.; RUMJANEK, V. M.; NOVOTNY, E. H.; OLIVARES, F. L.; SPACCINI, R.; PICCOLO, A. Chemical properties of humic matter as related to induction of plant lateral roots. European Journal of Soil Science, v. 63, n. 3, p. 315-324, Jun. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 32. | | AGUIAR, N. O.; OLIVARES, F. L.; NOVOTNY, E. H.; DOBBS, L. B.; BALMORI, D. M.; SANTOS JUNIOR, L. G.; CHAGAS, J. G.; FAÇANHA, A. R.; CANELLAS, L. P. Bioactivity of humic acids isolated from vermicomposts at different maturation stages. Plant and Soil, v. 362, p. 161-174, 2013.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Solos. |
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| 33. | | AGUIAR, N. O.; CANELLAS, L. P.; OLIVARES, F. L.; BUSATO, J. G.; SILVA, L. G. JR. S.; NOVOTNY, E. H.; FAÇANHA, A. R. Bioactivity of humic acids from vermicompost at increasing maturity stages. In: MEETING OF THE INTERNATIONAL HUMIC SUBSTANCES SOCIETY, 15., 2010, Tenerife. Proceedings... Tenerife: IHSS, 2010. v. 3, p. 332-335.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 34. | | BODDEY, R. M.; OLIVEIRA, O. C. de; URQUIAGA, S.; REIS, V. M.; OLIVARES, F. L. de; BALDANI, V. L. D.; DOBEREINER, Johanna. Biological nitrogen fixation associated with sugar cane and rice: Contributions and prospects for improvement. Plant and Soil, Netherlands, v. 174, p. 195-209, 1995.| Biblioteca(s): Embrapa Agrobiologia. |
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| 36. | | BALDANI, J. I.; POT, B.; KIRCHHOF, G.; FALSEN, E.; BALDANI, V. L. D.; OLIVARES, F. L.; HOSTE, B.; KERSTERS, K.; HARTMANN, A.; GILLIS, M.; DOBEREINER, J. Emended description of Herbaspirillum; inclusion of [Pseudomonas] rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. International Journal of Systematic Bacteriology, Washington, v. 46, n. 3, p. 802-810, 1996.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 37. | | AQUINO, A. M. de; CANELLAS, L. P.; SILVA, A. P. S. da; CANELLAS, N. O.; LIMA, L. da S.; OLIVARES, F. L.; PICCOLO, A.; SPACCINI, R. Evaluation of molecular properties of humic acids from vermicompost by 13 C CPMAS NMR spectroscopy and thermochemolysis GC MS Journal of Analytical and Applied Pyrolysis, v .141, 104634, August 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 38. | | BALDANI, J. I.; AZEVEDO, M. S. de; REIS, V. M.; TEIXEIRA, K. R. dos S.; OLIVARES, F. L.; GOI, S. R.; BALDANI, V. L. D.; DOBEREINER, Johanna. Fixacao biologica de nitrogenio em gramineas: Avancos e aplicacoes. In: SIQUEIRA, J.O.; MOREIRA, F.M.S.; LOPES, A.S.; GUILHERME, L.R.; FAQUIN, V.; FURTINNI, A.E.; CARVALHO, J.G., eds. Inter-Relacao fertilidade, biologia do solo e nutricao de plantas. Vicosa: SBCS; Lavras: UFLA/DCS, 1999. p.621-666.| Biblioteca(s): Embrapa Agrobiologia. |
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| 39. | | JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001.| Biblioteca(s): Embrapa Agrobiologia. |
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| 40. | | CANELLAS, L. P.; MÉDICI, L. O.; AGUIAR, N. O.; CAMPOSTRINI, E.; ROSA, R. C. C.; FAÇANHA, A. R.; OLIVARES, F. L.; BALMORI, D. M. A combination of humic substances and Herbaspirillum seropedicae inoculation enhances the growth of maize (Zea mays L.). Plant Soil, August, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 49 | |
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