Data/hora: 20/06/2024 02:18:08

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 08/01/2010
Data da última atualização: | 15/01/2020
Autoria: | FERRARI, F.; ZANCA, A. S.; JORDÃO, H.; GIACHETTO, P. F.; OKURA, V. K.; MACCHERONI, W.; FORNI-MARTINS, E. R.
Afiliação: | FERNANDA FERRARI, CanaVialis S.A., IB/Unicamp; A. S. ZANCA, CanaVialis S.A.; H. JORDÃO, CanaVialis S.A.; POLIANA FERNANDA GIACHETTO, CNPTIA; V. K. OKURA, CanaVialis S.A.; W. MACCHERONI, CanaVialis S.A.; E. R. FORNI-MARTINS, IB/Unicamp.
Título: | Sugarcane BAC selection for BAC-FISH in currents hybrids varieties.
Ano de publicação: | 2009
Fonte/Imprenta: | In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009.
Páginas: | p. 96.
Idioma: | Inglês
Conteúdo: | In order to better understand the organization and support sugarcane breeding programs we are beginning a construction of a physical map using BAC-FISH. We began the study with six clones BACs available at Alellyx S.A. These BACs were pre-selected for specific promoters search project. Filters of the sugarcane BAC library and the clones were obtained from Clemson University Genomics Institutes (CUGI, Clemson, South Carolina). This BAC library was constructed from genomic DNA of the hybrid 'R570. BAC DNA was shotgun sub cloned and was sequenced using BigDye system and ABI Prism 3730 DNA Sequencer (Applied Biosystems). Sequence homology was determined by BLAST searches of the nucleotide sequence database at the National Center for Biotechnology Information (NCBI). We analyzed the BLAST results and we could classify these six BACs when compared with sorghum genome database.
Palavras-Chave: | BAC library; BAC-FISH.
Thesagro: | Cana de açúcar; Genética.
Thesaurus Nal: | Breeding; Genetics; Physical chromosome mapping; Sugarcane.
Categoria do assunto: | --
Registro original: | Embrapa Agricultura Digital (CNPTIA)

Exemplares:

CNPTIA - (14439-1) - UPC - RA - PP -  -  - 2010.00016 - DES
CNPTIA - (14439-2) - UPC - RA - DD -  -  -  - DIS