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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
24/10/2017 |
Data da última atualização: |
01/02/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CLEMENTE, Z.; SILVA, G. H. da; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. |
Afiliação: |
VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA. |
Título: |
Exploring the mechanisms of graphene oxide toxicity to zebrafish embryo. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Applied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454. |
Idioma: |
Inglês |
Conteúdo: |
Many applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8. |
Palavras-Chave: |
Zebrafish. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165499/1/RA-CastroVLSS-ISTA18-ART-2017-PA....pdf
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Marc: |
LEADER 02169nam a2200193 a 4500 001 2078089 005 2021-02-01 008 2017 bl uuuu u00u1 u #d 100 1 $aCLEMENTE, Z. 245 $aExploring the mechanisms of graphene oxide toxicity to zebrafish embryo.$h[electronic resource] 260 $aApplied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454.$c2017 520 $aMany applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8. 653 $aZebrafish 700 1 $aSILVA, G. H. da 700 1 $aMARTINEZ, D. S. T. 700 1 $aMAURER-MORELLI, C. V. 700 1 $aNUNES, M. C. S. 700 1 $aTHOMAZ, A. A. 700 1 $aCASTRO, V. L. S. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
27/02/2009 |
Data da última atualização: |
28/10/2013 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
CARVALO, H. W. L. de; CARDOSO, M. J.; ROCHA, L. M. P.; PACHECO, C. A. P.; OLIVEIRA, I. R. de; TABISA, J. N.; LIRA, M. A.; OLIVEIRA, E. A. S.; MACEDO, J. J. G. de; NASCIMENTO, M. M. A. do; SIMPLÍCIO, J. B.; COUTINHO, G. V.; BRITO, A. R. de M. B.; TAVARES, J. A.; TAVARES FILHO, J. J.; MELO, K. E. de O.; FEITOSA, L. F.; MENEZES, A. F.; RODRIGUES, C. S. |
Afiliação: |
Hélio Wilson Lemos de Carvalho, Embrapa Tabuleiros Costeiros; Milton José Cardoso, Embrapa Meion-Norte; Leonardo Melo Pereira Rocha, Embrapa Milho e Sorgo; Cleso Antônio Pereira Rocha, Embrapa Milho e Sorgo; Ivênio Rubens de Oliveira, Embrapa Tabuleiros. |
Título: |
Avaliação de híbridos de milho no nordeste brasileiro: ensaios realizados no ano agrícola 2007/2008. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Costeiros, 2008. |
Série: |
(Embrapa Tabuleiros Costeiros. Comunicado Técnico, 79). |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Híbrod; Nordeste. |
Thesagro: |
Milho. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATC-2009-09/20591/1/cot_79.pdf
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Marc: |
LEADER 01132nam a2200373 a 4500 001 1372538 005 2013-10-28 008 2008 bl uuuu u0uu1 u #d 100 1 $aCARVALO, H. W. L. de 245 $aAvaliação de híbridos de milho no nordeste brasileiro$bensaios realizados no ano agrícola 2007/2008.$h[electronic resource] 260 $aAracaju: Embrapa Tabuleiros Costeiros$c2008 490 $a(Embrapa Tabuleiros Costeiros. Comunicado Técnico, 79). 650 $aMilho 653 $aBrasil 653 $aHíbrod 653 $aNordeste 700 1 $aCARDOSO, M. J. 700 1 $aROCHA, L. M. P. 700 1 $aPACHECO, C. A. P. 700 1 $aOLIVEIRA, I. R. de 700 1 $aTABISA, J. N. 700 1 $aLIRA, M. A. 700 1 $aOLIVEIRA, E. A. S. 700 1 $aMACEDO, J. J. G. de 700 1 $aNASCIMENTO, M. M. A. do 700 1 $aSIMPLÍCIO, J. B. 700 1 $aCOUTINHO, G. V. 700 1 $aBRITO, A. R. de M. B. 700 1 $aTAVARES, J. A. 700 1 $aTAVARES FILHO, J. J. 700 1 $aMELO, K. E. de O. 700 1 $aFEITOSA, L. F. 700 1 $aMENEZES, A. F. 700 1 $aRODRIGUES, C. S.
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