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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/04/1999 |
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Data da última atualização: |
12/04/1999 |
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Autoria: |
BAURENS, F.-C.; NOYER, J.-L.; LANAUD, C.; LAGODA, P. J. L. |
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Afiliação: |
CIRAD. |
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Título: |
Use of competitive PCR to assay copy number of repetitive elements in banana |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Mol. Gen. Genet, v.253, p.57-64, 1996 |
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Idioma: |
Inglês |
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Conteúdo: |
Banana is one of the most important sub-tropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements. |
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Palavras-Chave: |
Copy number; DNA repetitivo; Musa-competitive PCR; Numero de copias; PCR competitivo; Repeated DNA. |
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Thesagro: |
Banana. |
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Thesaurus Nal: |
Musa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01422naa a2200253 a 4500
001 1648090
005 1999-04-12
008 1996 bl --- 0-- u #d
100 1 $aBAURENS, F.-C.
245 $aUse of competitive PCR to assay copy number of repetitive elements in banana
260 $c1996
520 $aBanana is one of the most important sub-tropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements.
650 $aMusa
650 $aBanana
653 $aCopy number
653 $aDNA repetitivo
653 $aMusa-competitive PCR
653 $aNumero de copias
653 $aPCR competitivo
653 $aRepeated DNA
700 1 $aNOYER, J.-L.
700 1 $aLANAUD, C.
700 1 $aLAGODA, P. J. L.
773 $tMol. Gen. Genet$gv.253, p.57-64, 1996
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 12 | |
| 1. |  | BILLOTTE, N.; RISTERUCCI, A. M.; BARCELOS, E.; NOYER, J. L.; AMBLARD, P.; BAURENS, F. C. Development, characterisation, and across-taxa utility of oil palm (Elaeis guineensis Jacq.) microsatellite markers. Genome, v. 44, p. 413-425, 2001.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
|    |
| 4. | | COURTOIS, B.; FILLOUX, D.; AHMADI, N.; NOYER, J.-L.; BILLOT, C.; GUIMARÃES, E. P. Uso de marcadores moleculares para el manejo de poblaciones de arroz mejoradas mediante la selección recurrente. In: GUIMARÃES, E. P. (Ed.). Mejoramiento poblacional, una alternativa para explorar los recursos genéticos del arroz en América Latina. Cali: CIAT, 2003. p. 53-76. (CIAT. Publicación, 337).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | DUVAL, M. F.; NOYER, J. L.; HAMON, P.; BUSO, G. C.; FERREIRA, F. R.; FERREIRA, M. E.; D’EECKENBRUGGE, C. Using chloroplast DNA markers to understand Ananas and Pseudomonas genetic diversity. Acta Horticulturae, n. 666, p. 93-107, Jan. 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | D'EECKENBRUGGE, G. C.; CABRAL, J. R. S.; CARLIER, J.; DUVAL, M. F.; FERREIRA, F. R.; LEAL, F.; LEITÃO, J.; MATOS, A. P. de; NOYER, J. L.; SUAREZ, Z. The EU-funded project 'Evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties. In: D'EECKENBRUGGE, G. C. (Coord.). Evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties (IC18-CT96-0118): annual scientific report 1998. [Montpellier]: CIRAD-FLHOR; [Rome]: IPGRI, 1998. paginação irregular. Communications on results from the project, presented at the Third International Pineapple Symposium. (November 17-20, Pattaya, Thailand).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | COPPENS d'EECKENBRUGGE, G.; CABRAL, J. R. S.; MATOS, A. P. de; CARLIER, J.; LEITÃO, J.; DUVAL, M. F.; NOYER, J. L.; FERREIRA, F. R.; LEAL, F.; MAGGIONI, L.; SUÁREZ, Z. Main results from the EU-funded project "Evaluation and utilization of pineapple genetic resources from Amazon to breed resistant varieties". Acta Horticulturae, n. 666, p. 77-82, Jan. 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | D'EECKENBRUGGE, G. C.; CABRAL, J. R. S.; CARLIER, J.; DUVAL, M. F.; FERREIRA, F. R.; LEAL, F.; LEITÃO, J.; MAGGIONI, L.; MATOS, A. P. de; NOYER, J. L.; SUÁREZ, Z. Main results from the EU-funded project 'Evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties'. In: D'EECKENBRUGGE, G. C. (Coord.). Evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties (IC18-CT96-0118): final scientific report. [Montpellier]: CIRAD-FLHOR; [Rome]: IPGRI, 1997. paginação irregular.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | D'EECKENBRUGGE, G. C.; CABRAL, J. R. S.; CARLIER, J.; DUVAL, M. F.; FERREIRA, F. R.; LEAL, F.; LEITÃO, J.; MAGGIONI, L.; MATOS, A. P. de; NOYER, J. L.; SUAREZ, Z. Main results from the EU-funded project 'evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties. In: D'EECKENBRUGGE, G. C. (Coord.). Evaluation and utilization of pineapple genetic resources from the Amazon to breed resistant varieties (IC18-CT96-0118): scientific annual report 2001. [Montpellier]: CIRAD-FLHOR; [Rome]: IPGRI, 2001. paginação irregular.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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