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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/08/2013 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; NOGUEIRA, E. O. P. L.; TINOCO, M. L. P.; FARIA, J. C. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, CENARGEN; ELSA OLIVEIRA P E LAGO NOGUEIRA, CENARGEN; MARIA LAINE P. TINOCO, CENARGEN; JOSIAS CORREA DE FARIA, CNPAF. |
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Título: |
Molecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Biotechnology, Amsterdam, v. 66, n. 1/2, p. 42-50, June 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.jbiotec.2013.04.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Golden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market. MenosGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the ... Mostrar Tudo |
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Thesagro: |
Engenharia genética; Feijão. |
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Thesaurus Nal: |
Begomovirus; Dry beans; Geminiviridae; Genetic engineering; RNA interference. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02424naa a2200253 a 4500
001 1968340
005 2023-03-08
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jbiotec.2013.04.009$2DOI
100 1 $aARAGÃO, F. J. L.
245 $aMolecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus.$h[electronic resource]
260 $c2013
520 $aGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market.
650 $aBegomovirus
650 $aDry beans
650 $aGeminiviridae
650 $aGenetic engineering
650 $aRNA interference
650 $aEngenharia genética
650 $aFeijão
700 1 $aNOGUEIRA, E. O. P. L.
700 1 $aTINOCO, M. L. P.
700 1 $aFARIA, J. C.
773 $tJournal of Biotechnology, Amsterdam$gv. 66, n. 1/2, p. 42-50, June 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 23 | |
| 8. |  | BONFIM, K.; FARIA, J. C.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. Plantas transgênicas de feijão obtidas através da estratégia de RNA interferente foram altamente resistentes ao Bean Golden Mosaic Virus. Summa Phytopathologica, v. 33, p. S.83, fev. 2007. Suplemento. Ref. 290. Edição dos Resumos do XXX Congresso Paulista de Fitopatologia, Jaboticabal, fev. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | FARIA, J. C.; BONFIM, K.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. Resistance to bean golden mosaic virus in genetically engineered common bean mediated by RNAi. In: ANNIVERSARY OF THE BEAN IMPROVEMENT COOPERATIVE, 50.; BIENNIAL, 19., 2007, Madison, Wisconsin. Program and abstracts. [S. l.]: Bean Improvement Cooperative, 2007. p. 15.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | FARIA, J. C.; BONFIM, K.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. Resistance to bean golden mosaic virus in genetically engineered common bean mediated by RNAi. In: ANNIVERSARY OF THE BEAN IMPROVEMENT COOPERATIVE, 50.; BIENNIAL, 19., 2007, Madison, Wisconsin. Program and abstracts. [S. l.]: Bean Improvement Cooperative, 2007. p. 15. Oral presentations.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 13. | | BONFIM, K.; FARIA, J. C.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. RNAi-mediated resistance to Bean golden mosaic virus genetically engineered common bean. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 5.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 3., 2007, Ouro Preto. Program e abstracts. Viçosa, MG: Universidade Federal de Viçosa, 2007. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | BONFIM, K.; FARIA, J. C. de; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. RNAi-mediated resistance to Bean golden mosaic virus genetically engineered common bean. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 5.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 3., 2007, Ouro Preto. Program e abstracts. Viçosa, MG: Universidade Federal de Viçosa, 2007. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 18. | | ALMEIDA, L. M.; CIPRIANO, T. M.; BONFIM, K.; NOGUEIRA, E. O. P. e L.; RIBEIRO, S. da G.; ARAGAO, F. J. L. Begomovirus resistance in transgenic tomatoes. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 303.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | ALMEIDA, L. M.; CIPRIANO, T. M.; BONFIM, K.; NOGUEIRA, E. O. P. e L.; RIBEIRO, S. da G.; ARAGAO, F. J. L. Resistência mediada por RNAi à begomovírus bipartidos em tomates geneticamente modificados. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 032.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | RIBEIRO, S. da G.; CIPRIANO, T.; NOGUEIRA, E. O. P. e L.; BONFIM, K.; TINOCO, M. L.; ARAGAO, F. J. L. RNAI-mediated resistance to bipartite begomoviruses in genetically modified tomatoes. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 9., 2009, St. Louis, Missouri. Proceedings... [S.l.]: The International Society for Plant Molecular Biology, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 23 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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