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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/12/2014 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FRESCHI, G. P. G.; NOGUEIRA, A. R. de A. |
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Afiliação: |
GIAN PAULO GIOVANNI FRESCHI, Universidade Federal de Alfenas; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Speciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Atomic Spectroscopy, v. 35, n. 5, p. 205-212, 2014. |
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Idioma: |
Português |
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Conteúdo: |
Selenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), respectively. The recoveries obtained were 96% for Se(IV), 98% for Se(VI), 91% for selenocystamine, 89% for seleno-cysteine, and 84% for seleno-methionine. MenosSelenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), re... Mostrar Tudo |
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Palavras-Chave: |
Bovine blood; Enzymatic estraction; Se. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113167/1/Binder2.pdf
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Marc: |
LEADER 02190naa a2200169 a 4500
001 2001777
005 2023-03-20
008 2014 bl uuuu u00u1 u #d
100 1 $aFRESCHI, G. P. G.
245 $aSpeciation of different forms of Se in bovine blood by enzymatic extraction and HPLC/Photo-Reduction/HG-ICP-OES.$h[electronic resource]
260 $c2014
520 $aSelenium is essential for the vital functions of the human and animal organism, such as growth, reproduction, prevention, and the protection of its muscular integrity. Although this dependence is well characterized, aspects related to selenium speciation still need to be studied further. This work describes the development of an alternative method for selenium speciation in bovine blood samples using a hyphenated analytical system (HPLC-UV-HG-ICP-OES). The influence of the main parameters related to the detection of Se species, such as separation efficiency, photo-reduction and hydride generation, were studied. Enzymatic reaction with proteinase-K (1 day at 37ºC) was employed for sample preparation. An on-line UV reduction system was adopted to convert the different species of Se to Se(IV). For the chromatographic system, equipped with an anionic exchange column, a phosphate-buffer of 40 mmol L-1 in the pH of 6.0 was used as the mobile phase (flow rate of 1 mL min-1 ) for separation of the different Se species. To increase the sensitivity, hydride generation was used, followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The developed method for Se speciation in bovine blood samples was performed completely on-line. The limits of detection (LODs) were 9.5, 7.2, 5.8, 3.1, 4.4 ?g L-1 and the limits of quantification (LOQs) were 31.4, 23.9, 20.1, 10.5, and 14.5 ?g L-1 for selenomethylselenocysteine, seleno-DL-methionine, Se(IV), and Se(VI), respectively. The recoveries obtained were 96% for Se(IV), 98% for Se(VI), 91% for selenocystamine, 89% for seleno-cysteine, and 84% for seleno-methionine.
653 $aBovine blood
653 $aEnzymatic estraction
653 $aSe
700 1 $aNOGUEIRA, A. R. de A.
773 $tAtomic Spectroscopy$gv. 35, n. 5, p. 205-212, 2014.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 4. |  | NOGUEIRA, A. R. de A. Preparo de amostras. In: ENCONTRO NACIONAL SOBRE MÉTODOS DOS LABORATÓRIOS DA EMBRAPA, 8., 2003, Jaguariúna, SP. Novas perspectivas para os laboratórios da Embrapa. Jaguariúna: Embrapa Meio Ambiente, 2003. 1 CD ROM.| Tipo: Capítulo em Livro Técnico-Científico |
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