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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
21/10/2004 |
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Data da última atualização: |
26/10/2004 |
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Autoria: |
SILVEIRA, V.; STEINER, N.; SANTOS, A. L. W. dos; NODARI, R. O.; GUERRA, M. P. |
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Título: |
Biotechnology tolls in Araucaria angustifolia conservation and improvement: inductive factors affecting somatic embryogenesis. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 3, p. 463-470, Sept. 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Biotechnological techniques based on somatic embryogenesis have been employed for capturing genetic gains in forest breeding programs. The present work aimed at studying the factors associated with the induction of somatic embryogenesis in Araucaria angustifolia. Zygotic embryos in different development stages were inoculated in a LP medium supplemented with 2,4-D, BA and KIN. Embryogenic cultures were inoculated in a LP liquid medium free of plant growth regulators. ABA and/or PEG 4000 were evaluated for the development of somatic embryos. The embryogenic induction was dependent on the genotype of the mother plant, ranging from 21.9% to 55.2%. Suspension cultures showed a 5.6 times increment of the initial cell volume after 33 days in the culture medium free of plant growth regulators. It was possible to establish and multiply autonomous embryogenic cultures. The medium containing ABA 50 (mM) and PEG (1%) resulted in the development o globular and torpedo somatic embryos. |
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Palavras-Chave: |
Autonomous; Brazilian pine; Conservation; Embriogênese somática; Improvement; Somatic; Suspension cultures. |
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Thesagro: |
Araucária Angustifólia; Melhoramento. |
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Thesaurus Nal: |
embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01863naa a2200289 a 4500
001 1212615
005 2004-10-26
008 2002 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, V.
245 $aBiotechnology tolls in Araucaria angustifolia conservation and improvement$binductive factors affecting somatic embryogenesis.
260 $c2002
520 $aBiotechnological techniques based on somatic embryogenesis have been employed for capturing genetic gains in forest breeding programs. The present work aimed at studying the factors associated with the induction of somatic embryogenesis in Araucaria angustifolia. Zygotic embryos in different development stages were inoculated in a LP medium supplemented with 2,4-D, BA and KIN. Embryogenic cultures were inoculated in a LP liquid medium free of plant growth regulators. ABA and/or PEG 4000 were evaluated for the development of somatic embryos. The embryogenic induction was dependent on the genotype of the mother plant, ranging from 21.9% to 55.2%. Suspension cultures showed a 5.6 times increment of the initial cell volume after 33 days in the culture medium free of plant growth regulators. It was possible to establish and multiply autonomous embryogenic cultures. The medium containing ABA 50 (mM) and PEG (1%) resulted in the development o globular and torpedo somatic embryos.
650 $aembryogenesis
650 $aAraucária Angustifólia
650 $aMelhoramento
653 $aAutonomous
653 $aBrazilian pine
653 $aConservation
653 $aEmbriogênese somática
653 $aImprovement
653 $aSomatic
653 $aSuspension cultures
700 1 $aSTEINER, N.
700 1 $aSANTOS, A. L. W. dos
700 1 $aNODARI, R. O.
700 1 $aGUERRA, M. P.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 2, n. 3, p. 463-470, Sept. 2002.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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