Data/hora: 08/06/2024 10:26:20

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 23/06/2017
Data da última atualização: | 23/06/2017
Autoria: | CHOY, V. J.; NIXON, A. J.; EARSON, A. J.
Título: | Distribution of prolactin receptor immunoreactivity in ovine skin and changes during the wool follicle growth cycle.
Ano de publicação: | 1997
Fonte/Imprenta: | Journal of Endocrinology, v. 155, n. 2, p. 265-275, Nov. 1997.
Idioma: | Inglês
Conteúdo: | Abstract: Prolactin is believed to mediate seasonal cues entraining seasonal reproductive and hair follicle growth cycles. Prolactin receptor binding activity and prolactin receptor gene expression in mammalian skin have recently been described. In this report, prolactin receptor immunoreactivity is identified in sheep skin using a monoclonal antibody against the rat liver prolactin receptor. Western blotting analysis of microsomal membrane proteins from skin showed major bands corresponding to molecular weights of 87 and 71 kDa and minor bands at 101 and 21 kDa. RNase protection analysis revealed the presence of mRNA species coding for long and short forms of the prolactin receptor. Formalin-fixed sections, exposed to the monoclonal antibody and stained by an immunogold method, revealed prolactin receptor-immunoreactivity in the dermal papilla, germinal matrix, outer root sheath, lower regions of the inner root sheath and connective tissue sheath of wool follicles. Staining was absent from keratinised cell populations. In all samples, the interfollicular epidermis, sebaceous and sweat glands were positively stained. The distribution of prolactin receptor is described in both growing and inactive wool follicles and related to postulated cycle-specific actions of circulating prolactin in the control of seasonal fibre growth.
Palavras-Chave: | Growth control.
Thesagro: | RNA.
Thesaurus Nal: | Antibodies; Chemistry; Hormone receptors; Metabolism; Prolactin; Sebaceous Glands; Sheep; Skin glands; Wool.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (33948-1) - ADD - AP - PP -  -  -  - DIS