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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
07/07/2008 |
Data da última atualização: |
07/07/2008 |
Autoria: |
NISHI, C. Y. M.; BODDEY, L. H.; VARGAS, M. A. T.; HUNGRIA, M. |
Título: |
Morphological, physiological and genetic characterization of two new Bradyrhizobium strains recently recommended as Brazilian commercial inoculants for soybean. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
Symbiosis, Rehovot, v. 20, n. 2, p. 147-162, 1996. |
Idioma: |
Inglês |
Conteúdo: |
Large scale soybean inoculation in Brazil started in 1960 and, since then, several strains from other countries were introduced into the Brazilian soils. Since 1992, two new strains have been used as Brazilian inoculants, CPAC 7 and CPAC 15, which are natural variants of CB 1809 (received from Australia in 1966) and SEMIA 566 (isolated from a North American inoculant in 1966), respectively, and were obtained in a selection program for higher efficiency of N-2 fixation and competitiveness. Analyses of morphological and physiological parameters, particularly the ones that differentiate the species B. japonicum and B. elkanii, indicated that CB 1809 and the variant CPAC 7 show the characteristics of B. japonicum, while SEMIA 566 and CPAC 15 fit into the species B, elkanii. Differences between parental and variant strains included also several profiles in RAPD analysis. Increases in nodulation, nodule occupancy and yield due to inoculation were observed even in a soil containing 2.2 x 10(5) cells/g of soil. The evaluation of nodule occupancy is essential to confirm the benefits of inoculation and, since the newly-recommended strains belong to serogroups already established in most Brazilian soils, the RAPD technique seems to be a suitable technique to differentiate the parental from variant strains. |
Palavras-Chave: |
Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio; Inoculation; Serologia; Soybean. |
Thesagro: |
Bradyrhizobium Japonicum; Glycine Max; Inoculação; Nodulação; Soja. |
Thesaurus Nal: |
Bradyrhizobium elkanii; nodulation; serology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02296naa a2200337 a 4500 001 1629915 005 2008-07-07 008 1996 bl --- 0-- u #d 100 1 $aNISHI, C. Y. M. 245 $aMorphological, physiological and genetic characterization of two new Bradyrhizobium strains recently recommended as Brazilian commercial inoculants for soybean. 260 $c1996 520 $aLarge scale soybean inoculation in Brazil started in 1960 and, since then, several strains from other countries were introduced into the Brazilian soils. Since 1992, two new strains have been used as Brazilian inoculants, CPAC 7 and CPAC 15, which are natural variants of CB 1809 (received from Australia in 1966) and SEMIA 566 (isolated from a North American inoculant in 1966), respectively, and were obtained in a selection program for higher efficiency of N-2 fixation and competitiveness. Analyses of morphological and physiological parameters, particularly the ones that differentiate the species B. japonicum and B. elkanii, indicated that CB 1809 and the variant CPAC 7 show the characteristics of B. japonicum, while SEMIA 566 and CPAC 15 fit into the species B, elkanii. Differences between parental and variant strains included also several profiles in RAPD analysis. Increases in nodulation, nodule occupancy and yield due to inoculation were observed even in a soil containing 2.2 x 10(5) cells/g of soil. The evaluation of nodule occupancy is essential to confirm the benefits of inoculation and, since the newly-recommended strains belong to serogroups already established in most Brazilian soils, the RAPD technique seems to be a suitable technique to differentiate the parental from variant strains. 650 $aBradyrhizobium elkanii 650 $anodulation 650 $aserology 650 $aBradyrhizobium Japonicum 650 $aGlycine Max 650 $aInoculação 650 $aNodulação 650 $aSoja 653 $aBiological nitrogen fixation 653 $aBNF 653 $aFBN 653 $aFixação biológica de nitrogênio 653 $aInoculation 653 $aSerologia 653 $aSoybean 700 1 $aBODDEY, L. H. 700 1 $aVARGAS, M. A. T. 700 1 $aHUNGRIA, M. 773 $tSymbiosis, Rehovot$gv. 20, n. 2, p. 147-162, 1996.
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Embrapa Agrobiologia (CNPAB) |
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