|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
23/03/2005 |
|
Data da última atualização: |
18/03/2010 |
|
Autoria: |
WILBURN, B.; NIMS, S.; CAMMUSO, C.; MIDURA, P. OLIVER, A.; SMITH, T. E.; POLLOCK, D.; MEADE, H.; ZIOMEK, C.; ECHELARD, Y.; GAVIN, W. G. |
|
Título: |
Analysis of factors affecting embryo transfers in the production of transgenic goats. |
|
Ano de publicação: |
1998 |
|
Fonte/Imprenta: |
Theriogenology, v. 49, n. 1, p.397, 1998. |
|
Idioma: |
Inglês |
|
Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society Marriott at Copley Place, Boston, USA, jan. 1998. |
|
Conteúdo: |
The generation of transgenic goats by microinjection is an inefficient and labor intensive processo To determine factors that could affect the number of transgenic animaIs born after embryo transfer , data resulting from a microinjection program carried out during the Fall season (September through December) was compiled and analyzed.
Goat embryos were collected surgically from Alpine, Toggenburg, or Saanen does, previously superovulated and bred. Following microinjection with Goat Beta-Casein DNA constructs (at a final concentration of 2-4 microgram/ml), embryos were transferred surgically to recipient does synchronized with their contemporary donors, usually plus or minus 24 hrs. Pregnancy was determined by ultrasound scanning (dy 35,60) and does were allowed to kid naturally. The overall pregnancy rate observed in this study was 55% of 123 recipients receiving a total of 490 microinjected embryos. The first analysis was the number of embryos transferred per recipient. Transfers of 3-,4-,5-, and 6-embryos were compared (n=51,41,35,7 respectively). Results show that the highest percentage of kids born per embryo transferred is obtained with 3-embryos (27.5%). This was supported by the finding that the rate of multiple kid births from a single recipient did not increase greatly when 4-, 5-, and 6- embryo transfers were compared to 3-embryo transfers (23.5%, 50%, 50% versus 41.7%). This suggested that increasing the number of embryos transferred to a recipient did not significantly increase the frequencies of twins or triplets from a single recipient but conceivably wasted valuable microinjected embryos. It was also found that pregnancy rates of animaIs in each transfer group (3-, 4-, 5-, 6-embryos) were approximately the same. An analysis of 1-sided and 2-sided embryo transfers was also performed. During 1-sided embryo transfers, embryos are implanted in only one oviduct. This procedure was performed, when only one of the recipient does ovaries had a corpus luteum (CL). Our analysis indicated that one-sided 3-embryo transfers showed the highest kid return per transferred embryo (31.0%). The history of a recipient, including the number of previous surgeries, as well as the multiparity was also considered. The number of surgeries was important since surgeries can cause adhesions that could affect pregnancy rates. However, an increasing number of previous surgeries did not seem to significantly decrease pregnancies resulting from microinjected embryo transfers. On the other hand, previous pregnancies influenced kid production. Multiparous recipients showed an increased (by 20-25%) rate of twin and triplet births when compared to nulliparous recipients. This seems to indicate that multiparous animaIs mar more easily carry a larger number of microinjected embryos to term. In summary, analysis of embryo transfer data during a goat transgenic program indicated that 3-embryo transfers to multiparous recipient does gave the highest return of kids per microinjected embryo transferred. MenosThe generation of transgenic goats by microinjection is an inefficient and labor intensive processo To determine factors that could affect the number of transgenic animaIs born after embryo transfer , data resulting from a microinjection program carried out during the Fall season (September through December) was compiled and analyzed.
Goat embryos were collected surgically from Alpine, Toggenburg, or Saanen does, previously superovulated and bred. Following microinjection with Goat Beta-Casein DNA constructs (at a final concentration of 2-4 microgram/ml), embryos were transferred surgically to recipient does synchronized with their contemporary donors, usually plus or minus 24 hrs. Pregnancy was determined by ultrasound scanning (dy 35,60) and does were allowed to kid naturally. The overall pregnancy rate observed in this study was 55% of 123 recipients receiving a total of 490 microinjected embryos. The first analysis was the number of embryos transferred per recipient. Transfers of 3-,4-,5-, and 6-embryos were compared (n=51,41,35,7 respectively). Results show that the highest percentage of kids born per embryo transferred is obtained with 3-embryos (27.5%). This was supported by the finding that the rate of multiple kid births from a single recipient did not increase greatly when 4-, 5-, and 6- embryo transfers were compared to 3-embryo transfers (23.5%, 50%, 50% versus 41.7%). This suggested that increasing the number of embryos transferred to a recipient did not sig... Mostrar Tudo |
|
Palavras-Chave: |
Transgênico. |
|
Thesagro: |
Caprino; Reprodução Animal; Transferência de Embrião. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03925naa a2200289 a 4500
001 1531011
005 2010-03-18
008 1998 bl uuuu u00u1 u #d
100 1 $aWILBURN, B.
245 $aAnalysis of factors affecting embryo transfers in the production of transgenic goats.
260 $c1998
500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society Marriott at Copley Place, Boston, USA, jan. 1998.
520 $aThe generation of transgenic goats by microinjection is an inefficient and labor intensive processo To determine factors that could affect the number of transgenic animaIs born after embryo transfer , data resulting from a microinjection program carried out during the Fall season (September through December) was compiled and analyzed. Goat embryos were collected surgically from Alpine, Toggenburg, or Saanen does, previously superovulated and bred. Following microinjection with Goat Beta-Casein DNA constructs (at a final concentration of 2-4 microgram/ml), embryos were transferred surgically to recipient does synchronized with their contemporary donors, usually plus or minus 24 hrs. Pregnancy was determined by ultrasound scanning (dy 35,60) and does were allowed to kid naturally. The overall pregnancy rate observed in this study was 55% of 123 recipients receiving a total of 490 microinjected embryos. The first analysis was the number of embryos transferred per recipient. Transfers of 3-,4-,5-, and 6-embryos were compared (n=51,41,35,7 respectively). Results show that the highest percentage of kids born per embryo transferred is obtained with 3-embryos (27.5%). This was supported by the finding that the rate of multiple kid births from a single recipient did not increase greatly when 4-, 5-, and 6- embryo transfers were compared to 3-embryo transfers (23.5%, 50%, 50% versus 41.7%). This suggested that increasing the number of embryos transferred to a recipient did not significantly increase the frequencies of twins or triplets from a single recipient but conceivably wasted valuable microinjected embryos. It was also found that pregnancy rates of animaIs in each transfer group (3-, 4-, 5-, 6-embryos) were approximately the same. An analysis of 1-sided and 2-sided embryo transfers was also performed. During 1-sided embryo transfers, embryos are implanted in only one oviduct. This procedure was performed, when only one of the recipient does ovaries had a corpus luteum (CL). Our analysis indicated that one-sided 3-embryo transfers showed the highest kid return per transferred embryo (31.0%). The history of a recipient, including the number of previous surgeries, as well as the multiparity was also considered. The number of surgeries was important since surgeries can cause adhesions that could affect pregnancy rates. However, an increasing number of previous surgeries did not seem to significantly decrease pregnancies resulting from microinjected embryo transfers. On the other hand, previous pregnancies influenced kid production. Multiparous recipients showed an increased (by 20-25%) rate of twin and triplet births when compared to nulliparous recipients. This seems to indicate that multiparous animaIs mar more easily carry a larger number of microinjected embryos to term. In summary, analysis of embryo transfer data during a goat transgenic program indicated that 3-embryo transfers to multiparous recipient does gave the highest return of kids per microinjected embryo transferred.
650 $aCaprino
650 $aReprodução Animal
650 $aTransferência de Embrião
653 $aTransgênico
700 1 $aNIMS, S.
700 1 $aCAMMUSO, C.
700 1 $aMIDURA, P. OLIVER, A.
700 1 $aSMITH, T. E.
700 1 $aPOLLOCK, D.
700 1 $aMEADE, H.
700 1 $aZIOMEK, C.
700 1 $aECHELARD, Y.
700 1 $aGAVIN, W. G.
773 $tTheriogenology$gv. 49, n. 1, p.397, 1998.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
