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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/04/1996 |
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Data da última atualização: |
03/10/2016 |
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Autoria: |
HERMOZO DE MENDOZA, J.; NIETO, C. G.; ARENAS, A.; ALONSO, J. M.; REY, J.; GIL, M. C.; ANTON, J.; CARDENAL, A.; HERMOSO DE MENDONZA, M. |
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Título: |
An indirect fluorescent antibody technique for detection of anti-dermatophilus congelensis antibodies in sheep. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Tropical Animal Health and Production, Edinburgh, v. 26, n. 2, p. 74-78, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
An indirect fiuorescent antibody (IFA) technique has been developed for detection of anti-Dermatophilus antibodies in sheep. Sera from 25 bacteriologically confirmed clinically affected sheep and from 10 negative non affected lambs were used. Whole cell antigen from brain heart infusion cultures of D. congolensis was used and all sera were tested in the same way for cross-reactivity against antigens obtained from cultures of Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides, and Corynebacterium pseudotuberculosis. Sera from Dermatophilus-infected sheep gave positive results with D. congolensis antigen and negative results with the antigens from other bacteria. The whole cell antigens employed were simple to prepare and easy to recognise by microscopy. Cross-reactivity was further tested using the D. tongolensis culture whole cell antigen and 3 sera from sheep with bacteriologically confirmed natural infections due to Corynebacterium pseudotuberculosis, Actinomyces pyogenes and Nocardia asteroides. None of these sera showed positive reactions. The authors recommend this technique for serological surveys and research on dermatophilosis. |
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Palavras-Chave: |
Dermatofilose. |
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Thesagro: |
Doença Animal; Ovino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01901naa a2200253 a 4500
001 1516379
005 2016-10-03
008 1994 bl uuuu u00u1 u #d
100 1 $aHERMOZO DE MENDOZA, J.
245 $aAn indirect fluorescent antibody technique for detection of anti-dermatophilus congelensis antibodies in sheep.
260 $c1994
520 $aAn indirect fiuorescent antibody (IFA) technique has been developed for detection of anti-Dermatophilus antibodies in sheep. Sera from 25 bacteriologically confirmed clinically affected sheep and from 10 negative non affected lambs were used. Whole cell antigen from brain heart infusion cultures of D. congolensis was used and all sera were tested in the same way for cross-reactivity against antigens obtained from cultures of Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides, and Corynebacterium pseudotuberculosis. Sera from Dermatophilus-infected sheep gave positive results with D. congolensis antigen and negative results with the antigens from other bacteria. The whole cell antigens employed were simple to prepare and easy to recognise by microscopy. Cross-reactivity was further tested using the D. tongolensis culture whole cell antigen and 3 sera from sheep with bacteriologically confirmed natural infections due to Corynebacterium pseudotuberculosis, Actinomyces pyogenes and Nocardia asteroides. None of these sera showed positive reactions. The authors recommend this technique for serological surveys and research on dermatophilosis.
650 $aDoença Animal
650 $aOvino
653 $aDermatofilose
700 1 $aNIETO, C. G.
700 1 $aARENAS, A.
700 1 $aALONSO, J. M.
700 1 $aREY, J.
700 1 $aGIL, M. C.
700 1 $aANTON, J.
700 1 $aCARDENAL, A.
700 1 $aHERMOSO DE MENDONZA, M.
773 $tTropical Animal Health and Production, Edinburgh$gv. 26, n. 2, p. 74-78, 1994.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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