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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
28/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. |
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Afiliação: |
TATIANE ALMEIDA DRUMMOND TETZNER, UNESP/JABOTICABAL; NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA-USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTNA RAMIRES FERREIRA, UNICAMP/CAMPINAS; CLARA SLADE OLIVEIRA, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011. |
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DOI: |
https://doi.org/10.1590/S1516-35982011001000010 |
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Idioma: |
Inglês |
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Conteúdo: |
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. MenosEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryo; Protein source. |
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Thesaurus Nal: |
in vitro culture; ovalbumin. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49368/1/PROCI2011.00188.pdf
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Marc: |
LEADER 02467naa a2200253 a 4500
001 1908631
005 2022-07-28
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1516-35982011001000010$2DOI
100 1 $aTETZNER, T. A. D.
245 $aThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos.$h[electronic resource]
260 $c2011
520 $aEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
650 $ain vitro culture
650 $aovalbumin
653 $aBovine embryo
653 $aProtein source
700 1 $aSARAIVA, N. Z.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aFERREIRA, C. R.
700 1 $aOLIVEIRA, C. S.
700 1 $aGARCIA, J. M.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 10, p. 2135-2141, oct. 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
11/01/2013 |
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Data da última atualização: |
11/01/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANGNES, G.; OLIVEIRA, P. A. V. de; MILLER, P. R. M. |
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Afiliação: |
GRACIELE ANGNES, UFSC; PAULO ARMANDO VICTORIA DE OLIVEIRA, CNPSA; PAUL RICHARD M. MILLER, UFSC. |
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Título: |
Emissão de gases em sistema de compostagem usado no tratamento de dejetos de suínos. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 41., 2012, Londrina. A engenharia agrícola na evolução dos sistemas de produção: anais Londrina: Sbea, 2012. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
Projeto/Plano de Ação: 01.10.06.001 |
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Palavras-Chave: |
Emissão de gás. |
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Thesagro: |
Compostagem; Dejeto; Suíno. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00703nam a2200181 a 4500
001 1944759
005 2013-01-11
008 2012 bl uuuu u00u1 u #d
100 1 $aANGNES, G.
245 $aEmissão de gases em sistema de compostagem usado no tratamento de dejetos de suínos.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 41., 2012, Londrina. A engenharia agrícola na evolução dos sistemas de produção: anais Londrina: Sbea, 2012. 1 CD-ROM.$c2012
500 $aProjeto/Plano de Ação: 01.10.06.001
650 $aCompostagem
650 $aDejeto
650 $aSuíno
653 $aEmissão de gás
700 1 $aOLIVEIRA, P. A. V. de
700 1 $aMILLER, P. R. M.
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Embrapa Suínos e Aves (CNPSA) |
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