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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/06/2011 |
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Data da última atualização: |
27/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MINGOTI, G. Z.; CASTRO, V. S. D. C.; NICIURA, S. C. M.; BARRETO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
GISELE ZOCCAL MINGOTI, UNESP/ARAÇATUBA; VIVIANE SGGOBI DIAS CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; LETÍCIA SIQUEIRA SÁ BARETTO, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In Vitro Cell Dev Biol Anim., v. 47, n. 5-6, p.361-367, jun. 2011. |
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DOI: |
10.1007/s11626-011-9400-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Aiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2. MenosAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addit... Mostrar Tudo |
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Palavras-Chave: |
Embryo; In vitro maturation; Macromolecule. |
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Thesaurus Nal: |
beef cattle; oxygen. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02490naa a2200241 a 4500
001 1894336
005 2022-07-27
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s11626-011-9400-0$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development.$h[electronic resource]
260 $c2011
520 $aAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2.
650 $abeef cattle
650 $aoxygen
653 $aEmbryo
653 $aIn vitro maturation
653 $aMacromolecule
700 1 $aCASTRO, V. S. D. C.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tIn Vitro Cell Dev Biol Anim.$gv. 47, n. 5-6, p.361-367, jun. 2011.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 21. |  | NUCCI, A. DA S.; NICIURA, S. C. M.; FELIPELLI, G.; OKINO, C. H.; MALAGO JUNIOR, W. Genotipagem por PCR de deleção no éxon 11 do gene mptl-1 associada à resistência ao monepantel em Haemonchus contortus. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2021. p.23. (Embrapa Pecuária Sudeste. Documentos, 140).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 22. | | NUCCI, A. DA S.; FELIPPELLI, G.; OKINO, C. H.; NICIURA, S. C. M. Genotipagem por sondas de hidrólise em qPCR de deleção no éxon 11 do gene mptl-1 potencialmente associada à resistência ao monepantel em Haemonchus contortus. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 14., 2022, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2022. p. 11. (Embrapa Instrumentação. Documentos, 73).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 23. | | IBELLI, A. M. G.; REGITANO, L. C. de A.; NICIURA, S. C. M. Extração de RNA. In: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007. p. 9-11.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 32. | | ALEMÁN GAINZA, Y.; FANTATTO, R. R.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Determination of parasite resistance status to anthelmintics through the larval development test. In: ENCONTRO CIENTÍFICO DE PRODUÇÃO ANIMAL SUSTENTÁVEL, 7., 2017, Campinas, SP . Anais... Nova Odessa, SP: Instituto de Zootecnia, 2017. Boletim de Indústria Animal, v. 74.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 36. | | MARGATHO, L. F. F.; NICIURA, S. C. M.; VERISSIMO, C. J.; MOLENTO, M. B. Situação da resistência à anti-helmínticos em um rebanho no município de Piratinga, SP, Brasil. O Biológico, av. 71, n. 2, p. 119, 2009| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 39. | | ROCHA, M. I. P.; MELLO, S. de C.; GROMBONI, J. G. G.; OTSUK, I. P.; NICIURA, S. C. M. Frequência do polimorfismo F200Y no gene da b-tubulina em em rebanhos ovinos do Estado de São Paulo. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 40. | | OLIVEIRA, A. de L.; ROCHA, M. I. P.; MELLO, S. S.; BUZATTO, V. C.; NICIURA, S. C. M. Estabelecimento de cultura celular primária de mioblastos de bovinos com diferentes genótipos para um SNP no gene CAST: resultados preliminares. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 4., 2012, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2012. p. 36. (Embrapa Instrumentação. Documentos, 56).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 174 | |
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