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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus Nal: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI
100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 174 | |
| 121. |  | OKINO, C. H.; NICIURA, S. C. M.; GIARETTA, P. R.; MELITO, G.; KAPRITCHKOFF, R. T. I.; SANTOS, I. B.; RECH, R. R.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Improved mucosal response against hemonchosis related to Beta A allele of ovine beta-globin gene. In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 13., 2023, Satellite Meeting. Programme & Abstracts... Kruger Park/South Africa: IVIS, 2023. p. 54-55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 122. | | OKINO, C. H.; TOSCANO, J. H. B.; LOPES, L. G.; SILVA, M. H.; GIRALDELO, L. A.; NICIURA, S. C. M.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. Immune cell profiles associated with resistance against Haemonchus contortus in Morada Nova sheep. In: ANNUAL MEETING OF THE EUROPEAN FEDERATION OF ANIMAL SCIENCE, 69., 2018, Dubrovnik, Croatia. Proceedings... Dubrovnik, Croatia: Wageningen Academic Publishers, 2018. p.310. 27-31 august 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 123. | | ROCHA, M. I. P.; SOUZA, M. M. de; DINIZ, W. J. S.; LIMA, A. O. de; AFONSO, J.; TIZIOTO, P. C.; OLIVEIRA, P. N. de; REGITANO, L. C. de A.; KOLTES, J. E.; NICIURA, S. C. M. Liver DNA methylation profile in Nelore cattle extremes for feed efficiency. In: WORKSHOP DO PROGRAMA DE PÓS-GRADUAÇÃO EM GENÉTICA EVOLUTIVA E BIOLOGIA MOLECULAR, 1., 2019, São Carlos, SP. Anais... São Carlos: UFSCAR, 2019. p.64| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 124. | | GARCIA, A. R.; MACHADO, R.; ESTEVES, S. N.; REGITANO, L. C. de A.; NICIURA, S. C. M.; BERGAMASCHI, M. A. C. M.; SANTANA, R. C. M.; PEREIRA, V. S. do A. Contribuição atual da Embrapa Pecuária Sudeste ao desenvolvimento da andrologia de ruminantes de interesse zootécnico. In: REUNIÃO DE ANDROLOGIA ANIMAL, 1., 2016, São Paulo. Anais... São Paulo: [s.n.], 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 125. | | CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S.; OKINO, C. H. Desenvolvimento de PCR para identificação de haplótipos da B-globina ovina e determinação de frequência alélica na raça Morada Nova . In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2021. p.21. (Embrapa Pecuária Sudeste. Documentos, 140).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 126. | | MOKRY, F. B.; LIMA, A. O.; MUDADU, M. A.; HIGA, R. H.; MEIRELLES, S. L. C.; SILVA, M. V. B.; CARDOSO, F. F.; NICIURA, S. C. M.; ALENCAR, M. M.; REGITANO, L. C. A. Descriptive analysis of haplotypes in a population of Canchim beef cattle. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçu: SBG, 2012. p. 1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sul. |
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| 127. | | MOKRY, F. B.; LIMA, A. O.; MUDADU, M. de A.; HIGA, R. H.; MEIRELLES, S. L. C.; SILVA, M. V. G. B.; CARDOSO, F. F.; NICIURA, S. C. M. Descriptive analysis of haplotypes in a population of Canchim beef cattle. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Anais... Foz do Igauaçu: SBG, 2012. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 128. | | SOUZA, M. M.; ZERLOTINI, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A. L.; MOKRY, F. B.; CESAR, A. S. M.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. A. Monoallelic expression of NNAT gene in Nelore steers skeletal muscle. In: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10.; 2014, Vancouver. Proceedings... Vancouver: American Society of Animal Science, 2014. Não paginado. WCGALP 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 129. | | CHAGAS, A. C. de S.; RIBEIRO, D. M.; OSÓRIO, H.; ABREU, A. A. P.; OKINO, C. H.; NICIURA, S. C. M.; AMARANTE, A. F. T.; BELLO, H. J. S.; MELITO, G. R.; ESTEVES, S. N.; ALMEIDA, A. M. Molecular signatures of Haemonchus contortus infection in sheep: a comparative serum proteomic study on susceptible and resistant sheep breeds. Veterinary Parasitology, v. 331, Oct. 2024, 110280. 11 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 130. | | BRUSCADIN, J. J.; SOUZA, M. M. DE; OLIVEIRA, K. S. DE; ROCHA, M. I. P.; AFONSO, J.; CARDOSO, T. F.; ZERLOTINI NETO, A.; COUTINHO, L. L.; NICIURA, S. C. M.; REGITANO, L. C. de A. Muscle allele-specifc expression QTLs may afect meat quality traits in Bos indicus. Scientific Reports, v.11, p.1-14, 2021. Article number: 7321.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 131. | | BRUSCADIN, J. J.; SOUZA, M. M. de; OLIVEIRA, K. S. de; ROCHA, M. I. P.; AFONSO, J.; CARDOSO, T. F.; ZERLOTINI NETO, A.; COUTINHO, L. L.; NICIURA, S. C. M.; REGITANO, L. C. de A. Muscle allele-specific expression QTLs may affect meat quality traits in Bos indicus. Scientific Reports, v. 11, p. 1-14, 2021. Article number: 7321.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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| 132. | | NICIURA, S. C. M.; CARDOSO, T. F.; IBELLI, A. M. G.; OKINO, C. H.; ANDRADE, B. G.; BENAVIDES, M. V.; CHAGAS, A. C. de S.; ESTEVES, S. N.; MINHO, A. P.; REGITANO, L. C. de A.; GONDRO, C. Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus. in sheep. Parasites & Vectors, v. 17, n. 102, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 133. | | OKINO, C. H.; NICIURA, S. C. M.; TOSCANO, J. H. B.; ESTEVES, S. N.; SANTOS, I. B. DOS; HAEHLING, M. B. VON; FIGUEIREDO, A.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Ovine B-globin gene: a new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. Veterinary Parasitology, v.294, june, 109434, 2021. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 134. | | OKINO, C. H.; BELLO, H. J. S.; KAPRITCHOKOFF, R. T. I.; MELITO, G. R.; FIORENTINO, M. I. M.; DONATONI, F. A. B.; NICIURA, S. C. M.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Ovine resistance against Haemonchus contortus-globin subtype feature? In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 13., 2023, Satellite Meeting. Programme & Abstracts... Kruger Park/South Africa: IVIS, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 135. | | ALEMÁN GAINZA, Y.; FANTATTO, R. R.; ALBUQUERQUE, A. C. A. de; AMARANTE, A. F. T. do; FERRAZ JUNIOR, R. S.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Padronização do teste de desenvolvimento larvar (TDL) para diagnóstico da resistência anti-helmíntica em Haemonchus contortus. São Carlos, SP: Embrapa Pecuária Sudeste, 2019. 22 p. (Embrapa Pecuária Sudeste. Boletim de Pesquisa e Desenvolvimento, 44).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 136. | | CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 137. | | NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, G. V.; GOMBRONI, J. G. G.; ROCHA, M. I. P.; SOUZA, M. M. de; MELLO, S. S. de; REGITANO, L. C. de A. Polymorphism and parent-of-origin effects on gene expression of CAST, leptin and DGAT1 in cattle. Meat Science, v. 90, n. 2, p. 507-510, feb. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 138. | | NICIURA, S. C. M.; ALBUQUERQUE, A. C. A. de; CRUVINEL, G. G.; MORAES, C.; CHAGAS, A. C. de S.; ESTEVES, S. N.; BENAVIDES, M. V.; AMARANTE, A. F. T. do. Preliminary genomic analyses of a brazillian isolate of Haemonchus contortus in a model for monepantel resistance. In: MOLECULAR AND CELLULAR BIOLOGY OF HELMINTHS, 12., 2018, Hydra, Greece. Proceedings... Hydra, Greece: [S.n], 2018. p.50. 2 - 7 September 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 139. | | MELITO, G. R.; BELLO, H. J. S.; KAPRITCHKOFF, R. T. I.; FIORENTINO, M. I. M.; NICIURA, S. C. M.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S.; OKINO, C. H. Quantificação da expressão de genes de resposta imune local em ovinos de diferentes haplótipos da beta-globina. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 15., 2023, São Carlos, SP. Anais [...]. São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2023. p. 24. (Embrapa Pecuária Sudeste. Eventos Técnicos & Científicos, 1)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 140. | | VERÍSSIMO, C; NICIURA, S. C. M.; BARBOSA, C. P.; CHIEBAO, D. P.; COSTA, R. D.; CURCI, V. M.; MOLENTO, M. B.; NARDON, R.; OLIVEIRA, D. J.; PEREIRA, J. R.; RODRIGUES, C. F.; SILVA, G. S.; UENO, T. H. Resistência múltipla de nematoides gastrintestinais parasitos de ovinos a anti-helmínticos no estado de São Paulo. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grande: CBPV, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 174 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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