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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/1999 |
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Data da última atualização: |
12/08/1999 |
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Autoria: |
VIERLING, R. A.; NGUYEN, H. T. |
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Afiliação: |
Plant Genetics Laboratory, Department of Agronomy, Horticulture and Entomology, Texas Tech University, Lubbock Texas 79409, USA. |
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Título: |
Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Theor Appl Genet, v.84, p.835-838, 1992 |
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Idioma: |
Inglês |
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Conteúdo: |
The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chhain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relatio-ships among genotypes. |
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Palavras-Chave: |
Genetic diversity; Phenograms; RAPD. |
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Thesaurus Nal: |
Triticum monococcum; Triticum urartu. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01279naa a2200193 a 4500
001 1648246
005 1999-08-12
008 1992 bl --- 0-- u #d
100 1 $aVIERLING, R. A.
245 $aUse of RAPD markers to determine the genetic diversity of diploid, wheat genotypes
260 $c1992
520 $aThe genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chhain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relatio-ships among genotypes.
650 $aTriticum monococcum
650 $aTriticum urartu
653 $aGenetic diversity
653 $aPhenograms
653 $aRAPD
700 1 $aNGUYEN, H. T.
773 $tTheor Appl Genet$gv.84, p.835-838, 1992
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
