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Registro Completo |
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
04/01/2021 |
Data da última atualização: |
18/02/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NEVES, L. V. de M. W.; FRACETTO, F. J. C.; FRACETTO, G. G. M.; ARAUJO FILHO, J. C. de; ARAUJO, J. K. S.; SANTOS, J. C. B. dos; MENDES JÚNIOR, J. P.; SOUZA JÚNIOR, V. D. de. |
Afiliação: |
LAÉRCIO VIEIRA DE MELO WANDERLEY NEVES, UFRPE; FELIPE JOSÉ CURY FRACETTO, UFRPE; GISELLE GOMES MONTEIRO FRACETTO, UFRPE; JOSE COELHO DE ARAUJO FILHO, CNPS; JANE KELLY SILVA ARAUJO, UFRPE; JEAN CHEYSON BARROS DOS SANTOS, UFRPE; JOSÉ PETRÔNIO MENDES JÚNIOR, UFRPE; VALDOMIRO SEVERINO DE SOUZA JÚNIOR, UFRPE. |
Título: |
Microbial abundance and C and N stocks in tropical degraded Planosols from semiarid northeastern Brazil. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Catena, v. 196, 104931, Jan. 2021. |
DOI: |
https://doi.org/10.1016/j.catena.2020.104931 |
Idioma: |
Inglês |
Conteúdo: |
Improper management and exploitation of the natural resources of the semiarid Planosols have triggered irreparable degradation processes in which vegetation can no longer reestablish itself naturally. We investigated preserved and degraded Planosols from Pernambuco State, Brazil, to determine total C and N stocks and quantify the functional and total microorganisms. We hypothesized that the degraded Planosol would have: i-significant reductions in C and N stocks, microbial biomass and activity in relation to the soil with preserved vegetation; ii-significant decreases in the proportion of total diazotrophic bacteria and archaea, ammonia-oxidizing bacteria, iron-reducing and -oxidizing bacteria and in alkaline phosphatase producers along the pedogenetic horizons. Thus, microbial biomass, soil basal respiration and metabolic and microbial quotients were obtained. Furthermore, total and functional gene copies were quantified by quantitative polymerase chain reaction (qPCR), and the functionality of biological nitrogen fixation (BNF) was determined using an acetylene reduction assay. In degraded Planosols, the activity from the present diazotrophs has been responsible for maintaining soil N stocks (i.e. soil N stocks without significant differences between the evaluated Planosols), but the soil C stocks were halved. Biomass and microbial activity did not show significant differences between two Planosols and there was no apparent metabolic stress from the identified microbiota. We found a significant proportion of structural and functional microorganisms in both Planosols. There were many archaea predominating in the Bt pedogenetic horizon in both Planosols, and a high proportion of microorganismal alkaline phosphatase was also found. We highlight the need for studies on BNF efficiency, especially focusing on archaea and free-living bacteria, as they are performing this important biological function, even in totally degraded semiarid soils. MenosImproper management and exploitation of the natural resources of the semiarid Planosols have triggered irreparable degradation processes in which vegetation can no longer reestablish itself naturally. We investigated preserved and degraded Planosols from Pernambuco State, Brazil, to determine total C and N stocks and quantify the functional and total microorganisms. We hypothesized that the degraded Planosol would have: i-significant reductions in C and N stocks, microbial biomass and activity in relation to the soil with preserved vegetation; ii-significant decreases in the proportion of total diazotrophic bacteria and archaea, ammonia-oxidizing bacteria, iron-reducing and -oxidizing bacteria and in alkaline phosphatase producers along the pedogenetic horizons. Thus, microbial biomass, soil basal respiration and metabolic and microbial quotients were obtained. Furthermore, total and functional gene copies were quantified by quantitative polymerase chain reaction (qPCR), and the functionality of biological nitrogen fixation (BNF) was determined using an acetylene reduction assay. In degraded Planosols, the activity from the present diazotrophs has been responsible for maintaining soil N stocks (i.e. soil N stocks without significant differences between the evaluated Planosols), but the soil C stocks were halved. Biomass and microbial activity did not show significant differences between two Planosols and there was no apparent metabolic stress from the identified microbiota. ... Mostrar Tudo |
Palavras-Chave: |
Genes Funcionais. |
Thesagro: |
Biomassa; Horizonte; Solo. |
Thesaurus Nal: |
Archaea; Microbial biomass; Soil horizons. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 02879naa a2200301 a 4500 001 2128963 005 2021-02-18 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.catena.2020.104931$2DOI 100 1 $aNEVES, L. V. de M. W. 245 $aMicrobial abundance and C and N stocks in tropical degraded Planosols from semiarid northeastern Brazil.$h[electronic resource] 260 $c2021 520 $aImproper management and exploitation of the natural resources of the semiarid Planosols have triggered irreparable degradation processes in which vegetation can no longer reestablish itself naturally. We investigated preserved and degraded Planosols from Pernambuco State, Brazil, to determine total C and N stocks and quantify the functional and total microorganisms. We hypothesized that the degraded Planosol would have: i-significant reductions in C and N stocks, microbial biomass and activity in relation to the soil with preserved vegetation; ii-significant decreases in the proportion of total diazotrophic bacteria and archaea, ammonia-oxidizing bacteria, iron-reducing and -oxidizing bacteria and in alkaline phosphatase producers along the pedogenetic horizons. Thus, microbial biomass, soil basal respiration and metabolic and microbial quotients were obtained. Furthermore, total and functional gene copies were quantified by quantitative polymerase chain reaction (qPCR), and the functionality of biological nitrogen fixation (BNF) was determined using an acetylene reduction assay. In degraded Planosols, the activity from the present diazotrophs has been responsible for maintaining soil N stocks (i.e. soil N stocks without significant differences between the evaluated Planosols), but the soil C stocks were halved. Biomass and microbial activity did not show significant differences between two Planosols and there was no apparent metabolic stress from the identified microbiota. We found a significant proportion of structural and functional microorganisms in both Planosols. There were many archaea predominating in the Bt pedogenetic horizon in both Planosols, and a high proportion of microorganismal alkaline phosphatase was also found. We highlight the need for studies on BNF efficiency, especially focusing on archaea and free-living bacteria, as they are performing this important biological function, even in totally degraded semiarid soils. 650 $aArchaea 650 $aMicrobial biomass 650 $aSoil horizons 650 $aBiomassa 650 $aHorizonte 650 $aSolo 653 $aGenes Funcionais 700 1 $aFRACETTO, F. J. C. 700 1 $aFRACETTO, G. G. M. 700 1 $aARAUJO FILHO, J. C. de 700 1 $aARAUJO, J. K. S. 700 1 $aSANTOS, J. C. B. dos 700 1 $aMENDES JÚNIOR, J. P. 700 1 $aSOUZA JÚNIOR, V. D. de 773 $tCatena$gv. 196, 104931, Jan. 2021.
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Embrapa Solos (CNPS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
09/12/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 5 |
Autoria: |
MELO, B. P. de; MOURA, S. M. de; MORGANTE, C. V.; PINHEIRO, D. H.; ALVES, N. S. F.; RODRIGUES-SILVA, P. L.; LOURENCO, I. T.; ANDRADE, R. V.; FRAGOSO, R. da R.; SA, M. F. G. de. |
Afiliação: |
BRUNO PAES DE MELO, INCT Plant Stress Biotech; STÉFANIE MENEZES DE MOURA, INCT Plant Stress Biotech; CAROLINA VIANNA MORGANTE, CPATSA; DANIELE HELOISA PINHEIRO; NAYARA SABRINA FREITAS ALVES, UFPF; PAOLO LUCAS RODRIGUES-SILVA, UCB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; ROSÂNGELA VIEIRA ANDRADE, UCB; RODRIGO DA ROCHA FRAGOSO, CPAC; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 5, n. 1, e2021005, 2021. |
DOI: |
https://doi.org/10.4322/biori.202105 |
Idioma: |
Inglês |
Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa. |
Conteúdo: |
Promoters are upstream gene regulatory sequences recognized by transcription factors (TFs) involved in controlling transcription initiation and progression. For modern crop improvement, the design of efficient gene constructs relies on promoter efficiency, tissue specificity, and other characteristics that allow the introgression of agronomically relevant traits to overcome biotic and abiotic stresses. Several constitutive viral promoters, such as pCaMV35S, remain widely employed in the transgenic plant generation, but their indiscriminate use leads to gene silencing triggering and metabolic penalties impacting plant fitness. The identification and functional characterization of plant-derived promoters can unveil alternatives to commonly used non-homologous promoters; however, knowledge over them remains limited, especially for crops. This review summarizes plant promoters used to drive foreign gene expression in homologous and heterologous systems, focusing on inducible soybean promoters from genes upregulated by different biotic stresses. Analyses of these soybean promoters revealed 22 coincident cis-acting elements that can be used for synthetic engineering promoters responsive to multiple biotic stresses and, therefore, efficiently drive gene expression, conferring desirable traits in transgenic soybean. In addition, we also revisited commercial and protected promoters to provide an update on soybean promoters and gain new insights into superior crops’ development. |
Palavras-Chave: |
Estresse biótico; Genética de plantas; Plant genetic; Plant promoters; Regulation; Soybean promoters; Stress-inducible; Transcription. |
Thesagro: |
Melhoramento Genético Vegetal; Soja. |
Thesaurus NAL: |
Engineering; Genes. |
Categoria do assunto: |
-- G Melhoramento Genético |
Marc: |
LEADER 02771naa a2200397 a 4500 001 2143931 005 2022-06-10 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4322/biori.202105$2DOI 100 1 $aMELO, B. P. de 245 $aRegulated promoters applied to plant engineering$ban insight over promising soybean promoters under biotic stress and their cis-elements.$h[electronic resource] 260 $c2021 500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa. 520 $aPromoters are upstream gene regulatory sequences recognized by transcription factors (TFs) involved in controlling transcription initiation and progression. For modern crop improvement, the design of efficient gene constructs relies on promoter efficiency, tissue specificity, and other characteristics that allow the introgression of agronomically relevant traits to overcome biotic and abiotic stresses. Several constitutive viral promoters, such as pCaMV35S, remain widely employed in the transgenic plant generation, but their indiscriminate use leads to gene silencing triggering and metabolic penalties impacting plant fitness. The identification and functional characterization of plant-derived promoters can unveil alternatives to commonly used non-homologous promoters; however, knowledge over them remains limited, especially for crops. This review summarizes plant promoters used to drive foreign gene expression in homologous and heterologous systems, focusing on inducible soybean promoters from genes upregulated by different biotic stresses. Analyses of these soybean promoters revealed 22 coincident cis-acting elements that can be used for synthetic engineering promoters responsive to multiple biotic stresses and, therefore, efficiently drive gene expression, conferring desirable traits in transgenic soybean. In addition, we also revisited commercial and protected promoters to provide an update on soybean promoters and gain new insights into superior crops’ development. 650 $aEngineering 650 $aGenes 650 $aMelhoramento Genético Vegetal 650 $aSoja 653 $aEstresse biótico 653 $aGenética de plantas 653 $aPlant genetic 653 $aPlant promoters 653 $aRegulation 653 $aSoybean promoters 653 $aStress-inducible 653 $aTranscription 700 1 $aMOURA, S. M. de 700 1 $aMORGANTE, C. V. 700 1 $aPINHEIRO, D. H. 700 1 $aALVES, N. S. F. 700 1 $aRODRIGUES-SILVA, P. L. 700 1 $aLOURENCO, I. T. 700 1 $aANDRADE, R. V. 700 1 $aFRAGOSO, R. da R. 700 1 $aSA, M. F. G. de 773 $tBiotechnology Research and Innovation$gv. 5, n. 1, e2021005, 2021.
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