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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/03/2023 |
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Data da última atualização: |
22/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOLINA, J. P.; COELHO, S. G.; BELLI, A. L.; NEVES, L. F. M.; MACHADO, F. S.; PEREIRA, L. G. R.; TOMICH, T. R.; CARVALHO, W. A.; DAIBERT, R. M. P.; REIS, D. R. de L.; COSTA, S. F.; VOORSLUYS, A. L.; JACOB, D. V.; CAMPOS, M. M. |
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Afiliação: |
JOANA P. CAMPOLINA, Universidade Federal de Minas Gerais; SANDRA GESTEIRA COELHO, Universidade Federal de Minas Gerais; ANNA LUIZA BELLI, Universidade Federal de Minas Gerais; LUIZ F. MARTINS NEVES, Universidade Federal de Minas Gerais; FERNANDA SAMARINI MACHADO, CNPGL; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; THIERRY RIBEIRO TOMICH, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; RAQUEL M. P. DAIBERT; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; SUELY F. COSTA, Universidade Federal de Lavras; ALESSANDRA L. VOORSLUYS, Adisseo; DAVID V. JACOB, Adisseo; MARIANA MAGALHAES CAMPOS, CNPGL. |
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Título: |
Potential benefits of a blend of essential oils on metabolism, digestibility, organ development and gene expression of dairy calves. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Scientific Reports, v. 13, article 3378, 2023. |
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DOI: |
https://doi.org/10.1038/s41598-023-30088-y |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate blood cells and metabolites, insulin-like growth factor-1 (IGF-1), digestibility, internal organs weight and histology, gene expression, and spleen cell proliferation of pre-weaned bull calves supplemented with a blend of essential oils in milk replacer (MR). Sixteen newborn Holstein?×?Gyr crossbred dairy bull calves, with body weight at birth of 33.3?±?3.7 kg, were housed in individual sand bedded pens, blocked by genetic composition, and randomly assigned to 1 of 2 treatments in a randomized complete block design: Control (CON, n?=?8) and blend of essential oils supplementation (BEO, n?=?8, 1 g/day/calf, Apex Calf, Adisseo, China). The commercial blend was composed by plant extracts derived from anise, cinnamon, garlic, rosemary, and thyme. Animals were fed 5 L of MR/day reconstituted at 15% (dry matter basis), divided into two equal meals. Water and starter were provided ad libitum. ß-hydroxybutyrate, urea, and glucose were evaluated weekly, IGF-1 was evaluated biweekly, and total blood cell count was performed every four weeks until the end of the trial at eight weeks of age. Feed samples were collected three times a week and polled for weekly analysis. Apparent total nutrient digestibility was determined from d 56 to 60 of age. On d 60?±?1, animals were euthanized for organ weight, histology, spleen cell proliferation, and intestinal gene expression analysis. Data were analyzed independently using linear mixed models using the REML method in the nlme package in R for continuous outcomes. A non-parametric test was used for ordered categorical outcomes using the Artools package in R. There were no differences between groups for blood evaluations, digestibility, gene expression, and a spleen cell proliferation assay. However, BEO calves presented a heavier pancreas, heavier intestines, bigger ileum villi, and higher cecum butyrate levels (P?MenosThe objective of this study was to evaluate blood cells and metabolites, insulin-like growth factor-1 (IGF-1), digestibility, internal organs weight and histology, gene expression, and spleen cell proliferation of pre-weaned bull calves supplemented with a blend of essential oils in milk replacer (MR). Sixteen newborn Holstein?×?Gyr crossbred dairy bull calves, with body weight at birth of 33.3?±?3.7 kg, were housed in individual sand bedded pens, blocked by genetic composition, and randomly assigned to 1 of 2 treatments in a randomized complete block design: Control (CON, n?=?8) and blend of essential oils supplementation (BEO, n?=?8, 1 g/day/calf, Apex Calf, Adisseo, China). The commercial blend was composed by plant extracts derived from anise, cinnamon, garlic, rosemary, and thyme. Animals were fed 5 L of MR/day reconstituted at 15% (dry matter basis), divided into two equal meals. Water and starter were provided ad libitum. ß-hydroxybutyrate, urea, and glucose were evaluated weekly, IGF-1 was evaluated biweekly, and total blood cell count was performed every four weeks until the end of the trial at eight weeks of age. Feed samples were collected three times a week and polled for weekly analysis. Apparent total nutrient digestibility was determined from d 56 to 60 of age. On d 60?±?1, animals were euthanized for organ weight, histology, spleen cell proliferation, and intestinal gene expression analysis. Data were analyzed independently using linear mixed models using the... Mostrar Tudo |
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Palavras-Chave: |
Células do baço; Expressão gênica; Metabólito. |
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Thesagro: |
Bezerro Leiteiro; Célula Sangüínea; Digestibilidade; Fator de Crescimento; Gado Leiteiro; Peso. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152106/1/Potential-benefts-of-a-blend-of-essential-oils.pdf
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Marc: |
LEADER 03756naa a2200397 a 4500
001 2152106
005 2023-08-22
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-023-30088-y$2DOI
100 1 $aCAMPOLINA, J. P.
245 $aPotential benefits of a blend of essential oils on metabolism, digestibility, organ development and gene expression of dairy calves.$h[electronic resource]
260 $c2023
520 $aThe objective of this study was to evaluate blood cells and metabolites, insulin-like growth factor-1 (IGF-1), digestibility, internal organs weight and histology, gene expression, and spleen cell proliferation of pre-weaned bull calves supplemented with a blend of essential oils in milk replacer (MR). Sixteen newborn Holstein?×?Gyr crossbred dairy bull calves, with body weight at birth of 33.3?±?3.7 kg, were housed in individual sand bedded pens, blocked by genetic composition, and randomly assigned to 1 of 2 treatments in a randomized complete block design: Control (CON, n?=?8) and blend of essential oils supplementation (BEO, n?=?8, 1 g/day/calf, Apex Calf, Adisseo, China). The commercial blend was composed by plant extracts derived from anise, cinnamon, garlic, rosemary, and thyme. Animals were fed 5 L of MR/day reconstituted at 15% (dry matter basis), divided into two equal meals. Water and starter were provided ad libitum. ß-hydroxybutyrate, urea, and glucose were evaluated weekly, IGF-1 was evaluated biweekly, and total blood cell count was performed every four weeks until the end of the trial at eight weeks of age. Feed samples were collected three times a week and polled for weekly analysis. Apparent total nutrient digestibility was determined from d 56 to 60 of age. On d 60?±?1, animals were euthanized for organ weight, histology, spleen cell proliferation, and intestinal gene expression analysis. Data were analyzed independently using linear mixed models using the REML method in the nlme package in R for continuous outcomes. A non-parametric test was used for ordered categorical outcomes using the Artools package in R. There were no differences between groups for blood evaluations, digestibility, gene expression, and a spleen cell proliferation assay. However, BEO calves presented a heavier pancreas, heavier intestines, bigger ileum villi, and higher cecum butyrate levels (P?<?0.05), demonstrating that the EO supplementation helped intestinal development and symbiotic bacteria. It was also observed in CON animals? heavier respiratory tract and a higher eosinophil count (P?<?0.05). Therefore, the organs where eosinophils are more active had a better response for BEO animals. No differences were found in the intestinal gene expression in the immune context. These results demonstrate that supplementing essential oils in MR could contribute to gut development and immune function. However, more research is needed to understand its impact on body development and define the best dosage and route of administration.
650 $aBezerro Leiteiro
650 $aCélula Sangüínea
650 $aDigestibilidade
650 $aFator de Crescimento
650 $aGado Leiteiro
650 $aPeso
653 $aCélulas do baço
653 $aExpressão gênica
653 $aMetabólito
700 1 $aCOELHO, S. G.
700 1 $aBELLI, A. L.
700 1 $aNEVES, L. F. M.
700 1 $aMACHADO, F. S.
700 1 $aPEREIRA, L. G. R.
700 1 $aTOMICH, T. R.
700 1 $aCARVALHO, W. A.
700 1 $aDAIBERT, R. M. P.
700 1 $aREIS, D. R. de L.
700 1 $aCOSTA, S. F.
700 1 $aVOORSLUYS, A. L.
700 1 $aJACOB, D. V.
700 1 $aCAMPOS, M. M.
773 $tScientific Reports$gv. 13, article 3378, 2023.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
24/01/2019 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAÚJO, A. C. C. de; SILVA, R. L. de O.; SILVA, J. B. da; OLIVEIRA, M. F. de; BEZERRA NETO, J. P.; ABURJAILE, F. F.; MELO, N. F. de; BENKO-ISEPPON, A. M. |
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Afiliação: |
ANDREIA CRISTINY CEZARINO DE ARAÚJO; ROBERTA LANE DE OLIVEIRA SILVA; JÉSSICA BARBOZA DA SILVA; MARIANNE FIRMINO DE OLIVEIRA; JOÃO PACÍFICO BEZERRA NETO; FLÁVIA FIGUEIRA ABURJAILE; NATONIEL FRANKLIN DE MELO, CPATSA; ANA MARIA BENKO-ISEPPON. |
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Título: |
Análise de expressão gênica diferencial de fatores de transcrição WRKY em videira sob estresse biótico. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 22., 2018, Natal. Anais... Natal: UFRN: Sociedade Brasileira de Genética Regional Nordeste, 2018. |
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Páginas: |
p. 154. |
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Idioma: |
Português |
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Conteúdo: |
O presente estudo se propôs a validar transcritos WRKY via PCR quantitativa em tempo real (RT-qPCR) em acessos de videira infectados com X. citri em três tempos distintos (90 minutos, 24 e 48 horas) após a inoculação com a bactéria. |
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Palavras-Chave: |
RT-qPCR; Videira. |
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Thesagro: |
Marcador Molecular; Uva; Vitis Vinifera; Xanthomonas Citri. |
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Thesaurus NAL: |
Vitis. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191268/1/Natoniel-2.pdf
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Marc: |
LEADER 01169nam a2200289 a 4500
001 2104722
005 2024-01-08
008 2018 bl uuuu u00u1 u #d
100 1 $aARAÚJO, A. C. C. de
245 $aAnálise de expressão gênica diferencial de fatores de transcrição WRKY em videira sob estresse biótico.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 22., 2018, Natal. Anais... Natal: UFRN: Sociedade Brasileira de Genética Regional Nordeste$c2018
300 $ap. 154.
520 $aO presente estudo se propôs a validar transcritos WRKY via PCR quantitativa em tempo real (RT-qPCR) em acessos de videira infectados com X. citri em três tempos distintos (90 minutos, 24 e 48 horas) após a inoculação com a bactéria.
650 $aVitis
650 $aMarcador Molecular
650 $aUva
650 $aVitis Vinifera
650 $aXanthomonas Citri
653 $aRT-qPCR
653 $aVideira
700 1 $aSILVA, R. L. de O.
700 1 $aSILVA, J. B. da
700 1 $aOLIVEIRA, M. F. de
700 1 $aBEZERRA NETO, J. P.
700 1 $aABURJAILE, F. F.
700 1 $aMELO, N. F. de
700 1 $aBENKO-ISEPPON, A. M.
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Embrapa Semiárido (CPATSA) |
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