Data/hora: 23/06/2024 00:52:20

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 22/08/2005
Data da última atualização: | 06/11/2006
Autoria: | PICOLI, E. A. de T.; ALFENAS, A. C.; DIAS, L. L. C.; NEVES, D. A.; OTONI, W. C.
Título: | In vitro morphogenesis of Eucalyptus grandis: effects of antibiotics on explants.
Ano de publicação: | 2005
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 2, p. 234-240, June 2005.
Idioma: | Inglês
Conteúdo: | The effects of increasing cefotaxime, carbenicillin and timentin concentrations (0, 150, 300, 450 and 600 mg/l) on the in vitro morphogenesis of E. grandis explants were evaluated. Carbenicillin and timentin increased the frequency of explant regenerating calluses and decreased necrosis up to 600 mg l-1; cefotaxime behaved similarly up to 300 mg l-1. At this level there was an increasing frequency of explants and calluses showing necrosis. Hypocotyl and cotyledon explants presented similar morphogenetic results. The results suggest that carbenicillin or timentin should be used in Eucalyptus tissue culture protocols to control endophytic bacteria for genetic transformation protocols in view of their positive effect on callogenesis.
Palavras-Chave: | Antibióticos; Cultura de tecidos vegetais; Plant tissue culture.
Thesagro: | Eucalipto; Eucalyptus Grandis; Morfogênese.
Thesaurus Nal: | antibiotics; morphogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (22912-1) - ADD - AP - -- - 631.05 - C - 2005.00385 - DIS