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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
22/11/2019 |
Data da última atualização: |
10/03/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIANA, C.; SERENO, M. J.; BERSOT, L. dos S.; KICH, J. D.; NERO, L. A. |
Afiliação: |
CIBELI VIANA, UFV; MALLU JAGNOW SERENO, UFPR; LUCIANO DOS SANTOS BERSOT, UFPR; JALUSA DEON KICH, CNPSA; LUIS AUGUSTO NERO, UFV. |
Título: |
Comparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Foodborne Pathogens and Disease, v. 17, n. 3, p. 194-201, 2020. |
DOI: |
10.1089/fpd.2019.2712 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering. MenosAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the except... Mostrar Tudo |
Thesagro: |
Bacteriologia; Cadeia Produtiva; Carne; Elisa; Enzima; Salmonella; Suíno; Técnica Imunoenzimática. |
Thesaurus Nal: |
Bacteriology; Disease surveillance; Enzyme-linked immunosorbent assay; Isolation techniques; Pork. |
Categoria do assunto: |
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Marc: |
LEADER 02918naa a2200337 a 4500 001 2114901 005 2020-03-10 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1089/fpd.2019.2712$2DOI 100 1 $aVIANA, C. 245 $aComparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain.$h[electronic resource] 260 $c2020 520 $aAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering. 650 $aBacteriology 650 $aDisease surveillance 650 $aEnzyme-linked immunosorbent assay 650 $aIsolation techniques 650 $aPork 650 $aBacteriologia 650 $aCadeia Produtiva 650 $aCarne 650 $aElisa 650 $aEnzima 650 $aSalmonella 650 $aSuíno 650 $aTécnica Imunoenzimática 700 1 $aSERENO, M. J. 700 1 $aBERSOT, L. dos S. 700 1 $aKICH, J. D. 700 1 $aNERO, L. A. 773 $tFoodborne Pathogens and Disease$gv. 17, n. 3, p. 194-201, 2020.
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Embrapa Suínos e Aves (CNPSA) |
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4. | | MORAES, G. M. D. de; ABREU, L. R. de; EGITO, A. S. do; SALLES, H. O.; SILVA, L. M. F. da; NERO, L. A.; SANTOS, K. M. O. dos. Functional properties of Lactobacillus mucosae strains isolated from Brazilian goat milk. Probiotics and Antimicrobial Proteins, v. 9, n. 3, p. 235-245, Sept. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Caprinos e Ovinos. |
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