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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
11/04/2000 |
Data da última atualização: |
15/10/2004 |
Autoria: |
NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B.; CASAGRANDE, E. |
Título: |
Gene expression in brazilian soybean genotypes tolerant to water deficit. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999. |
Páginas: |
p.439. |
Idioma: |
Inglês |
Conteúdo: |
Abiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies. MenosAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with c... Mostrar Tudo |
Palavras-Chave: |
Brasil; Soybean; Tolerancia a seca. |
Thesagro: |
Soja. |
Thesaurus Nal: |
Brazil; soil water deficit. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02965naa a2200241 a 4500 001 1461655 005 2004-10-15 008 1999 bl uuuu u00u1 u #d 100 1 $aNEPOMUCENO, A. L. 245 $aGene expression in brazilian soybean genotypes tolerant to water deficit. 260 $c1999 300 $ap.439. 520 $aAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies. 650 $aBrazil 650 $asoil water deficit 650 $aSoja 653 $aBrasil 653 $aSoybean 653 $aTolerancia a seca 700 1 $aNEUMAIER, N. 700 1 $aFARIAS, J. R. B. 700 1 $aCASAGRANDE, E. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registros recuperados : 600 | |
102. | | CHICARELI, L. S.; OLIVEIRA, M. C. N. de; POLIZEL, A.; NEPOMUCENO, A. L. A presença de Outliers interfere no Teste F e no teste de comparações múltiplas de médias. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 139-146. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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104. | | FARIAS, J. R. B.; NEPOMUCENO, A. L.; NEUMAIER, N.; TOBITA, S.; ALMEIDA, I. R. de. Restrições da disponibilidade hídrica à obtenção de elevados rendimentos de grãos de soja. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 32-33. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.Biblioteca(s): Embrapa Soja. |
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105. | | FARIAS, J. R. B.; NEUMAIER, N.; SINCLAIR, T. R.; NEPOMUCENO, A. L. Respostas de cultivares de soja ao termofotoperíodo. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 84-85. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.Biblioteca(s): Embrapa Soja. |
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108. | | NEPOMUCENO, A. L.; FARIAS, J. R. B.; NEUMAIER, N.; OYA, T.; CATTELAN, A. J.; DELATTRE, N. Estratégias para amenizar impactos decorrentes das adversidades climáticas. In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2001: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2002. p. 23-24. (Embrapa Soja. Documentos, 198).Biblioteca(s): Embrapa Soja. |
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110. | | NEUMAIER, N.; ORTIZ, C. A.; FARIAS, J. R. B.; OYA, T.; NEPOMUCENO. A. L. Estádio de desenvolvimento e tombamento fisiológico em plântulas de soja. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 24., 2002, São Pedro, SP. Resumos... Londrina: Embrapa Soja, 2002. p. 192-193. (Embrapa Soja. Documentos, 185).Biblioteca(s): Embrapa Soja. |
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114. | | FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L.; MARAUX, F.; VAL, W. M. C. Evaluation of soybean yield estimation by the model SARRA. In: INTERNATIONAL SYMPOSIUM ON SYSTEMS APPROACHES FOR AGRICULTURAL DEVELOPMENT (SAAD-III), 3., 1999, Lima, Peru. Methodologies for interdisciplinary multiple scale perspectives: participants manual. Lima: ICASA/CIP/UNALM/IFDC/ILRI, 1999. Não paginado. Resumo (IV-P-3).Biblioteca(s): Embrapa Soja. |
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115. | | OYA, T.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J, R, B.; TOBITA, S.; ITO, O. Evaluation and characterization of drought tolerance among brazilian soybean cultivars in the field. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 185. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.Biblioteca(s): Embrapa Soja. |
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116. | | KARAM, D.; VOLL, E.; GAZZIERO, D. L. P.; NEPOMUCENO, A. L. Estudo do crescimento de Desmodium purpureum isolado ou em convivencia com Glycine max L. Merril. Revista Brasileira de Fisiologia Vegetal, Sao Carlos, v. 5, n. 1, p. 97, jun. 1993. Resumo apresentado no IV Congresso Brasileiro de Fisiologia Vegetal, 1993, Fortaleza.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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118. | | RAKOCEVIC, M.; NEUMAIER, N.; OLIVEIRA, G. M. de; NEPOMUCENO, A. L.; FARIAS, J. R. B. Heliotropic responses of soybean cultivars at three phenological stages and under two water regimes. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 661-670, jul. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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119. | | RAKOCEVIC, M.; NEUMAIER, N.; GOUVEIA, W. M. de; FARIAS, J. R. B.; NEPOMUCENO, A. L. Heliotropismo em soja para simulações de interceptação luminosa e fotossíntese. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p.121, trab. 208. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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120. | | NEPOMUCENO, A. L.; OOSTERHUIS, D.; STEWART, J. M.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton. Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 1, p.11-20, 2002.Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 600 | |
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