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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
21/08/2002 |
Data da última atualização: |
21/08/2002 |
Autoria: |
NEPOMUCENO, A. L.; OOSTERHUIS, D.; STEWART, J. M.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. |
Título: |
Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 1, p.11-20, 2002. |
Idioma: |
Inglês |
Conteúdo: |
Tolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit stress, however, all four genotypes were induced to express this homologue. Ribonuclease protection assays confirmed these results. This is an important finding since there are only few reports of trehalose presence in higher plants and none in cotton. MenosTolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit str... Mostrar Tudo |
Categoria do assunto: |
-- |
URL: |
https://www.scielo.br/pdf/bjpp/v14n1/11778.pdf
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Marc: |
LEADER 02321naa a2200181 a 4500 001 1444492 005 2002-08-21 008 2002 bl uuuu u00u1 u #d 100 1 $aNEPOMUCENO, A. L. 245 $aExpression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton. 260 $c2002 520 $aTolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit stress, however, all four genotypes were induced to express this homologue. Ribonuclease protection assays confirmed these results. This is an important finding since there are only few reports of trehalose presence in higher plants and none in cotton. 700 1 $aOOSTERHUIS, D. 700 1 $aSTEWART, J. M. 700 1 $aTURLEY, R. 700 1 $aNEUMAIER, N. 700 1 $aFARIAS, J. R. B. 773 $tBrazilian Journal of Plant Physiology, Londrina$gv. 14, n. 1, p.11-20, 2002.
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Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
13/09/2018 |
Data da última atualização: |
10/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
OLIVEIRA, T. A. de; LEMOS, W. de P.; TINÔCO, R. S.; BARBOSA, J. C.; MARTINS, I. C. F. |
Afiliação: |
Taciane Almeida de Oliveira, SUDAM; WALKYMARIO DE PAULO LEMOS, CPATU; Ricardo Salles Tinôco, Grupo Agropalma; José Carlos Barbosa, UNESP; Ivan Carlos Fernandes Martins, UFRA. |
Título: |
Validation of the sampling methodology for Opsiphanes invirae caterpillars on oil palm plantations in the Brazilian Amazon. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Ciência Rural, v. 48, n. 7, jul. 2018. |
DOI: |
10.1590/0103-8478cr20170742 |
Idioma: |
Inglês |
Conteúdo: |
Esta pesquisa validou a utilização da folha 17, nas amostragens de Opsiphanes invirae Hübner (Lepidoptera: Nymphalidae), através da quantificação da abundância de lagartas nas diferentes folhas que compõem os espirais em comparação com a folha 17, e, em diferentes regiões da folha (apical, intermediária e basal). O estudo foi realizado no Pará entre março de 2014 e março de 2015. Os resultados confirmam que a folha 17 é a mais adequada para ser utilizada no monitoramento desse desfolhador. |
Palavras-Chave: |
Desfolhador. |
Thesagro: |
Dendê; Elaeis Guineensis; Inseto; Lagarta. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182850/1/1678-4596-cr-48-07-e20170742.pdf
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Marc: |
LEADER 01233naa a2200241 a 4500 001 2095580 005 2019-01-10 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1590/0103-8478cr20170742$2DOI 100 1 $aOLIVEIRA, T. A. de 245 $aValidation of the sampling methodology for Opsiphanes invirae caterpillars on oil palm plantations in the Brazilian Amazon.$h[electronic resource] 260 $c2018 520 $aEsta pesquisa validou a utilização da folha 17, nas amostragens de Opsiphanes invirae Hübner (Lepidoptera: Nymphalidae), através da quantificação da abundância de lagartas nas diferentes folhas que compõem os espirais em comparação com a folha 17, e, em diferentes regiões da folha (apical, intermediária e basal). O estudo foi realizado no Pará entre março de 2014 e março de 2015. Os resultados confirmam que a folha 17 é a mais adequada para ser utilizada no monitoramento desse desfolhador. 650 $aDendê 650 $aElaeis Guineensis 650 $aInseto 650 $aLagarta 653 $aDesfolhador 700 1 $aLEMOS, W. de P. 700 1 $aTINÔCO, R. S. 700 1 $aBARBOSA, J. C. 700 1 $aMARTINS, I. C. F. 773 $tCiência Rural$gv. 48, n. 7, jul. 2018.
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