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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
15/01/2016 |
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Data da última atualização: |
19/01/2016 |
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Autoria: |
DROBNIS, E. Z.; NELSON, E. A.; LIN, T. Y. |
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Título: |
The effect of freezing rate on motility and GOT release of frozen goat spermatozoa. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON GOAT PRODUCTION AND DISEASE, 3., 1982, Tucson. Proceedings... Scottsdale: Dairy Goat Journal, 1982. p. 282 |
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ISBN: |
0-930848-17-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Twelve pooled semen samples from mature bucks were used. Split samples were washed once, then diluted to 400 x 10 6 spermatozoa/ml with Tris-citric acid yolk diluent. After cooling in 60 min. to 5¦C, an equal volume of glycerated diluent was added in three aliquots at 10 min. intervals. Final glycerol concentrations were 0 percent, 4 percent and 7 percent. Three freezing rates were used: slow, intermediate and fast; 30, 10, 7 min., respectively to -80¦C. Internal straw temperatures were measured. After freezing, straws were dropped directly into liquid nitrogen. Three equilibration rates included 0, 1 and 2 hours after glycerolization. Progressive motility and extracellular glutamic oxalacetic transaminase (GOT) were estimated at prefreeze, post-thaw and post-stress (3 hours post-thaw at 37¦C). Straws were thawed at 75¦C for 5 sec. There was a significant loss of glycerol. When negative effects on prefreeze motility were removed, equilibration resulted in a lower mean loss of motility between prefreeze and post-thaw. GOT release during freezing/thawing was lower for 2 h equilibration than for no holding time. This was most significant for 4 percent glycerol. An interaction between equilibration and freezing rate indicated that GOT release was greatest when semen was not equilibrated and fast frozen. Stress GOT release was higher for semen frozen just after glycerolization than for other treatments. Faster freezing rates resulted in less damage to the spermatozoa than slow rates . The only statiscally significant advantage for fast freezing was for post-thaw motility when 7 percent glycerol was used MenosTwelve pooled semen samples from mature bucks were used. Split samples were washed once, then diluted to 400 x 10 6 spermatozoa/ml with Tris-citric acid yolk diluent. After cooling in 60 min. to 5¦C, an equal volume of glycerated diluent was added in three aliquots at 10 min. intervals. Final glycerol concentrations were 0 percent, 4 percent and 7 percent. Three freezing rates were used: slow, intermediate and fast; 30, 10, 7 min., respectively to -80¦C. Internal straw temperatures were measured. After freezing, straws were dropped directly into liquid nitrogen. Three equilibration rates included 0, 1 and 2 hours after glycerolization. Progressive motility and extracellular glutamic oxalacetic transaminase (GOT) were estimated at prefreeze, post-thaw and post-stress (3 hours post-thaw at 37¦C). Straws were thawed at 75¦C for 5 sec. There was a significant loss of glycerol. When negative effects on prefreeze motility were removed, equilibration resulted in a lower mean loss of motility between prefreeze and post-thaw. GOT release during freezing/thawing was lower for 2 h equilibration than for no holding time. This was most significant for 4 percent glycerol. An interaction between equilibration and freezing rate indicated that GOT release was greatest when semen was not equilibrated and fast frozen. Stress GOT release was higher for semen frozen just after glycerolization than for other treatments. Faster freezing rates resulted in less damage to the spermatozoa than slow ra... Mostrar Tudo |
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Palavras-Chave: |
Preservação do sêmen; Semen preservation. |
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Thesagro: |
Caprino; Congelamento; Espermatozóide; Reprodução animal. |
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Thesaurus Nal: |
Goats; Reproduction; Spermatozoa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02437naa a2200265 a 4500
001 2034025
005 2016-01-19
008 1982 bl uuuu u00u1 u #d
020 $a0-930848-17-9
100 1 $aDROBNIS, E. Z.
245 $aThe effect of freezing rate on motility and GOT release of frozen goat spermatozoa.
260 $c1982
520 $aTwelve pooled semen samples from mature bucks were used. Split samples were washed once, then diluted to 400 x 10 6 spermatozoa/ml with Tris-citric acid yolk diluent. After cooling in 60 min. to 5¦C, an equal volume of glycerated diluent was added in three aliquots at 10 min. intervals. Final glycerol concentrations were 0 percent, 4 percent and 7 percent. Three freezing rates were used: slow, intermediate and fast; 30, 10, 7 min., respectively to -80¦C. Internal straw temperatures were measured. After freezing, straws were dropped directly into liquid nitrogen. Three equilibration rates included 0, 1 and 2 hours after glycerolization. Progressive motility and extracellular glutamic oxalacetic transaminase (GOT) were estimated at prefreeze, post-thaw and post-stress (3 hours post-thaw at 37¦C). Straws were thawed at 75¦C for 5 sec. There was a significant loss of glycerol. When negative effects on prefreeze motility were removed, equilibration resulted in a lower mean loss of motility between prefreeze and post-thaw. GOT release during freezing/thawing was lower for 2 h equilibration than for no holding time. This was most significant for 4 percent glycerol. An interaction between equilibration and freezing rate indicated that GOT release was greatest when semen was not equilibrated and fast frozen. Stress GOT release was higher for semen frozen just after glycerolization than for other treatments. Faster freezing rates resulted in less damage to the spermatozoa than slow rates . The only statiscally significant advantage for fast freezing was for post-thaw motility when 7 percent glycerol was used
650 $aGoats
650 $aReproduction
650 $aSpermatozoa
650 $aCaprino
650 $aCongelamento
650 $aEspermatozóide
650 $aReprodução animal
653 $aPreservação do sêmen
653 $aSemen preservation
700 1 $aNELSON, E. A.
700 1 $aLIN, T. Y.
773 $tIn: INTERNATIONAL CONFERENCE ON GOAT PRODUCTION AND DISEASE, 3., 1982, Tucson. Proceedings... Scottsdale: Dairy Goat Journal, 1982. p. 282
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Embrapa Caprinos e Ovinos (CNPC) |
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| 3. |  | JOSHI, L. R.; MOHR, K. A.; CLEMENT, T.; HAIN, K. S.; MYERS, B.; YAROS, J.; NELSON, E. A.; HENNINGS, J. C; GAVA, D.; SCHAEFER, R.; CARON, L.; DEE, S.; DIEL, D. G. Detection of the emerging picornavirus senecavirus a in pigs, mice, and houseflies. Journal of Clinical Microbiology, v. 54, n. 6, p. 1536-1545, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 3 | |
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