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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/09/2005 |
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Data da última atualização: |
28/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMANO, E.; SOARES, A.; PROITE, K.; NEIVA, S.; GROSSI, M.; FARIA, J. C.; RECH, E. L.; ARAGÃO, F. J. L. |
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Afiliação: |
EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; ALEXANDRE SOARES; KARINA PROITE; SUZANA NEIVA; MAIRA GROSSI; JOSIAS CORREA DE FARIA, CNPAF; ELIBIO LEOPOLDO RECH FILHO, Cenargen; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
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Título: |
Transgene elimination in genetically modified dry bean and soybean lines. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 4, n. 2, p. 177-184, 2005. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Transgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. More than 300 plants of progeny were obtained during this period, demonstrating that the phenomenon of elimination was consistently repeated and offering an opportunity for detailed study of transgene elimination, including the characterization of the integration sites. Cloning and sequencing of the transgenic loci, reciprocal crosses to untransformed plants, genomic DNA blots, and GUS assays were performed in the transgenic lines. Based on the molecular and genetic characterization, possible mechanisms involved in transgene elimination include intrachromosomal recombination, genetic instability resulting from the tissue culture manipulations, and co-elimination of transgenes, triggered by a process of genome defense. |
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Palavras-Chave: |
BIOLOGICAL; Transgene elimination. |
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Thesagro: |
Feijão; Phaseolus Vulgaris. |
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Thesaurus Nal: |
biolistics. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220180/1/gmr2005.pdf
https://www.funpecrp.com.br/gmr/year2005/vol2-4/pdf/gmr0118.pdf
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Marc: |
LEADER 01942naa a2200277 a 4500
001 1214084
005 2023-03-28
008 2005 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aROMANO, E.
245 $aTransgene elimination in genetically modified dry bean and soybean lines.$h[electronic resource]
260 $c2005
520 $aTransgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. More than 300 plants of progeny were obtained during this period, demonstrating that the phenomenon of elimination was consistently repeated and offering an opportunity for detailed study of transgene elimination, including the characterization of the integration sites. Cloning and sequencing of the transgenic loci, reciprocal crosses to untransformed plants, genomic DNA blots, and GUS assays were performed in the transgenic lines. Based on the molecular and genetic characterization, possible mechanisms involved in transgene elimination include intrachromosomal recombination, genetic instability resulting from the tissue culture manipulations, and co-elimination of transgenes, triggered by a process of genome defense.
650 $abiolistics
650 $aFeijão
650 $aPhaseolus Vulgaris
653 $aBIOLOGICAL
653 $aTransgene elimination
700 1 $aSOARES, A.
700 1 $aPROITE, K.
700 1 $aNEIVA, S.
700 1 $aGROSSI, M.
700 1 $aFARIA, J. C.
700 1 $aRECH, E. L.
700 1 $aARAGÃO, F. J. L.
773 $tGenetics and Molecular Research$gv. 4, n. 2, p. 177-184, 2005.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 5 | |
| 1. |  | SOARES, A.; NEIVA, S.; PROITE, K.; ARAGÃO, F.; ROMANO, E. Eliminação específica de transgenes em feijão. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 29.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | SOARES, A.; ROMANO, E.; PROITE, K.; VILARINHO, A. C.; NEIVA, S. RECH, E.; ARAGÃO, F. Análise de seqüências nucleotídicas de trangenes originados por biobalística revelam uma taxa extremamente baixa de mutação pontual. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2001. p. 27.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | NEIVA, S.; ROMANO, E.; SOARES, A.; PROITE, K.; VILARINHO, A. C.; RECH, E.; ARAGÃO, F. Análise de seqüências de soja transgênica sugere que cópias múltiplas do transgene pode ter origem pela replicação do dna exógeno. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2001. p. 26.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | SOARES, A.; ROMANO, E.; NEIVA, S.; DE CAPDEVILLE, G.; VIANNA, G. R.; RECH, E. L.; ARAGÃO, F. J. L. Inheritance of a recessive transgene-associated character controlling albinism in transgenic bean (Phaseolus vulgaris L.). Plant Biology, v. 7, p. 104-108, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | ROMANO, E.; SOARES, A.; PROITE, K.; NEIVA, S.; GROSSI, M.; FARIA, J. C.; RECH, E. L.; ARAGÃO, F. J. L. Transgene elimination in genetically modified dry bean and soybean lines. Genetics and Molecular Research, v. 4, n. 2, p. 177-184, 2005.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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