Data/hora: 25/06/2024 10:57:39

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 24/09/2004
Data da última atualização: | 12/07/2010
Autoria: | REGIS, L.; RIBEIRO, M.; VALE, V.; PAULE, B.; NASCIMENTO, I.; SCHAER, R.; MEYER, R.; FREIRE, S.
Título: | Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells.
Ano de publicação: | 2002
Fonte/Imprenta: | Small Ruminant Research, v. 46, n. 1, p. 71-73, 2002.
Idioma: | Inglês
Conteúdo: | ln the present study we determined the effect of  the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell membrane. Pokeweed and phytohemagglutinin showed a high stimulating effect, with proliferation rates of 16,753 and 2350 counts/min (cpm), respectively, whereas Con A showed very low stimulation indices (495 cpm), supporting the notion that it should not be used for blast proliferation studies in goats.
Palavras-Chave: | Bahia; Brasil; Con A; PHA; Proliferação; PWM.
Thesagro: | Cabra; Caprino; Célula; Lectina.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (17132-1) - ADC - AP - -- -  -  - 46,p.71 - DIS