| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
13/02/2017 |
|
Data da última atualização: |
04/12/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, N. MACHADO DOS; NASCIMENTO, C. W. A. do; ACCIOLY, A. M. de A. |
|
Afiliação: |
NIELSON MACHADO DOS SANTOS, UFRPE; CLÍSTENES WILLIAMS ARAÚJO DO NASCIMENTO, UFRPE; ADRIANA MARIA DE AGUIAR ACCIOLY, CNPMF. |
|
Título: |
Guideline Values and Metal Contamination in Soils of an Environmentally Impacted Bay |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Water, Air and Soil Pollution, p.228-88, 2017. |
|
ISSN: |
1573-2932 |
|
DOI: |
10.1007/s11270-017-3279-7 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Estimating soil guideline values for metals in environmentally impacted areas is challenging due to the contamination spreading. All Saints Bay holds the most important petrochemical complex in the southern hemisphere and an abandoned Pb smelter plant that left tons of slag rich in Pb, Cd, and Zn untreated. The aim of the study was to determine the background concentration of the metals Pb, Cr, Zn, Cu, Ni, Cd, and Co in topsoils of this environmentally impacted bay in order to establish soil guideline values and pollution indices for the monitoring of metal contamination. A total of 114 soil samples across the bay were taken from 38 samplings sites with minimal human activity. Concentrations of Pb, Cr, Zn, Cu, Ni, Cd, and Co in extracts were determined by inductively coupled plasma optical emission spectroscopy after microwave digestion. Soil guideline values, geo-accumulation, and pollution index were estimated. The natural concentration of Pb in soils was clearly influenced by smelting activities and hindered the establishment of a soil guidelines value for lead. Geo-accumulation data indicated that soils are contaminated with Pb, Co, Ni, Zn, and Cu. High pollution indices for Co, Pb, and Ni in the vicinity of the abandoned Pb plant smelter demonstrate that soils were influenced by this anthropogenic source of metals. Soils around the biggest city in the bay were also contaminated with Pb and chromium. |
|
Thesagro: |
Solo. |
|
Thesaurus Nal: |
Soil. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02005naa a2200193 a 4500
001 2063878
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
022 $a1573-2932
024 7 $a10.1007/s11270-017-3279-7$2DOI
100 1 $aSANTOS, N. MACHADO DOS
245 $aGuideline Values and Metal Contamination in Soils of an Environmentally Impacted Bay$h[electronic resource]
260 $c2017
520 $aEstimating soil guideline values for metals in environmentally impacted areas is challenging due to the contamination spreading. All Saints Bay holds the most important petrochemical complex in the southern hemisphere and an abandoned Pb smelter plant that left tons of slag rich in Pb, Cd, and Zn untreated. The aim of the study was to determine the background concentration of the metals Pb, Cr, Zn, Cu, Ni, Cd, and Co in topsoils of this environmentally impacted bay in order to establish soil guideline values and pollution indices for the monitoring of metal contamination. A total of 114 soil samples across the bay were taken from 38 samplings sites with minimal human activity. Concentrations of Pb, Cr, Zn, Cu, Ni, Cd, and Co in extracts were determined by inductively coupled plasma optical emission spectroscopy after microwave digestion. Soil guideline values, geo-accumulation, and pollution index were estimated. The natural concentration of Pb in soils was clearly influenced by smelting activities and hindered the establishment of a soil guidelines value for lead. Geo-accumulation data indicated that soils are contaminated with Pb, Co, Ni, Zn, and Cu. High pollution indices for Co, Pb, and Ni in the vicinity of the abandoned Pb plant smelter demonstrate that soils were influenced by this anthropogenic source of metals. Soils around the biggest city in the bay were also contaminated with Pb and chromium.
650 $aSoil
650 $aSolo
700 1 $aNASCIMENTO, C. W. A. do
700 1 $aACCIOLY, A. M. de A.
773 $tWater, Air and Soil Pollution, p.228-88, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Cerrados. |
|
Data corrente: |
20/09/2022 |
|
Data da última atualização: |
08/12/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
STEPHAN, M. P.; COSTA, A. M.; AZEVEDO, T. de L.; ROSA, J. S. da; SANTOS, A. A. dos. |
|
Afiliação: |
MARILIA PENTEADO STEPHAN, CTAA; ANA MARIA COSTA, CPAC; TATIANA DE LIMA AZEVEDO, CTAA; JEANE SANTOS DA ROSA, CTAA; ALEXSANDRO ARAUJO DOS SANTOS, CTAA. |
|
Título: |
Electrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Journal of Agricultural Science and Technology B, v. 12, n. 2, p. 40-47, 2022. |
|
DOI: |
https://doi.org/10.17265/2161-6264/2022.02.002 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Brazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them. MenosBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as ... Mostrar Tudo |
|
Palavras-Chave: |
Bioactive peptides; Mass distribution on fruit; P setacea; P tenuifila. |
|
Thesagro: |
Casca; Eletroforese; Fruto; Maracujá; Método de Análise; Peptídeo; Polpa; Proteína; Purificação; Rastreamento; Semente; Tecnologia de Alimento. |
|
Thesaurus NAL: |
Analytical methods; Bioactive compounds; Food technology; Fruits; Passiflora; Passion fruits; Peptides; Polyacrylamide gel electrophoresis; Proteins; Pulp; Purification methods; Seeds. |
|
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146660/1/62ea112170472.pdf
|
|
Marc: |
LEADER 03088naa a2200517 a 4500
001 2146660
005 2022-12-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.17265/2161-6264/2022.02.002$2DOI
100 1 $aSTEPHAN, M. P.
245 $aElectrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.$h[electronic resource]
260 $c2022
520 $aBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them.
650 $aAnalytical methods
650 $aBioactive compounds
650 $aFood technology
650 $aFruits
650 $aPassiflora
650 $aPassion fruits
650 $aPeptides
650 $aPolyacrylamide gel electrophoresis
650 $aProteins
650 $aPulp
650 $aPurification methods
650 $aSeeds
650 $aCasca
650 $aEletroforese
650 $aFruto
650 $aMaracujá
650 $aMétodo de Análise
650 $aPeptídeo
650 $aPolpa
650 $aProteína
650 $aPurificação
650 $aRastreamento
650 $aSemente
650 $aTecnologia de Alimento
653 $aBioactive peptides
653 $aMass distribution on fruit
653 $aP setacea
653 $aP tenuifila
700 1 $aCOSTA, A. M.
700 1 $aAZEVEDO, T. de L.
700 1 $aROSA, J. S. da
700 1 $aSANTOS, A. A. dos
773 $tJournal of Agricultural Science and Technology B$gv. 12, n. 2, p. 40-47, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
