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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sul. |
Data corrente: |
01/09/2021 |
Data da última atualização: |
01/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
COSTA, R. J. da; SILVA, A. P. da; FONSECA, R. N. da; HÜBNER, S. de O.; NALERIO, E. S.; MARQUES, J. de L.; VITOLA, H. R. S.; SILVA, W. P. da; DUVAL, E. H.; FIORENTINI, A. M. |
Afiliação: |
ROGER JUNGES DA COSTA, UFPEL; ANDRESA PEREIRA DA SILVA, IFSUL; RENATA NOBRE DA FONSECA, UFPEL; SILVIA DE OLIVEIRA HÜBNER, UFPEL; ELEN SILVEIRA NALERIO, CPPSUL; JULIANA DE LIMA MARQUES, UFPEL; HELENA REISSIG SOARES VITOLA, UFPEL; WLADIMIR PADILHA DA SILVA, UFPEL; EDUARDA HALLAL DUVAL, UFPEL; ANGELA MARIA FIORENTINI, UFPEL. |
Título: |
Characterization of Enterococcus faecium EO1 isolated from mutton and activity of bacteriocin-like substances in the control of Listeria monocytogenes in fresh mutton sausage. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Food Science and Technology, v. 141, 110954, Apr. 2021. |
DOI: |
https://doi.org/10.1016/j.lwt.2021.110954 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to isolate and characterize bacteriocinogenic lactic acid bacteria (LAB) from mutton carcasses after slaughter. The study also evaluated the activity of bacteriocin-like substances (BLS) for control of Listeria monocytogenes in fresh mutton sausage. The cell-free supernatant (CFS) was obtained and its antimicrobial activity was quantified. The presence of genes encoding enterocin was evaluated by molecular techniques. Another aspect assessed was the action in situ of BLS produced by these isolates for control of Listeria monocytogenes in fresh mutton sausage, experimentally contaminated. Six isolates presented phenotypic and genotypic characteristics of safety. Also, the antibacterial peptides present in the CFS showed stability at different temperatures, pH, NaCl and cure salt concentrations, as well as potential against L. monocytogenes Scott A. The isolate identified as Enterococcus faecium EO1 showed gene coding for the production of enterocin A and produced bacteriocin after 6 h of growth (400 AU. mL 1). Based on this, the CFS of EO1 (5 and 10%) was applied in fresh mutton sausage, being significantly effective for control of L. monocytogenes during 21 days of refrigerated storage (4.07 log CFU. g 1). Therefore, the potential for preservation of food has been demonstrated through the use of CFS containing bacteriocin-like substances from E. faecium EO1 in fresh mutton sausage. |
Palavras-Chave: |
Bioconservação; Derivado de carne. |
Thesagro: |
Carne Tratada; Carneiro; Listeria Monocytogenes; Ovino. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02468naa a2200313 a 4500 001 2133982 005 2021-09-01 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.lwt.2021.110954$2DOI 100 1 $aCOSTA, R. J. da 245 $aCharacterization of Enterococcus faecium EO1 isolated from mutton and activity of bacteriocin-like substances in the control of Listeria monocytogenes in fresh mutton sausage.$h[electronic resource] 260 $c2021 520 $aThe aim of this study was to isolate and characterize bacteriocinogenic lactic acid bacteria (LAB) from mutton carcasses after slaughter. The study also evaluated the activity of bacteriocin-like substances (BLS) for control of Listeria monocytogenes in fresh mutton sausage. The cell-free supernatant (CFS) was obtained and its antimicrobial activity was quantified. The presence of genes encoding enterocin was evaluated by molecular techniques. Another aspect assessed was the action in situ of BLS produced by these isolates for control of Listeria monocytogenes in fresh mutton sausage, experimentally contaminated. Six isolates presented phenotypic and genotypic characteristics of safety. Also, the antibacterial peptides present in the CFS showed stability at different temperatures, pH, NaCl and cure salt concentrations, as well as potential against L. monocytogenes Scott A. The isolate identified as Enterococcus faecium EO1 showed gene coding for the production of enterocin A and produced bacteriocin after 6 h of growth (400 AU. mL 1). Based on this, the CFS of EO1 (5 and 10%) was applied in fresh mutton sausage, being significantly effective for control of L. monocytogenes during 21 days of refrigerated storage (4.07 log CFU. g 1). Therefore, the potential for preservation of food has been demonstrated through the use of CFS containing bacteriocin-like substances from E. faecium EO1 in fresh mutton sausage. 650 $aCarne Tratada 650 $aCarneiro 650 $aListeria Monocytogenes 650 $aOvino 653 $aBioconservação 653 $aDerivado de carne 700 1 $aSILVA, A. P. da 700 1 $aFONSECA, R. N. da 700 1 $aHÜBNER, S. de O. 700 1 $aNALERIO, E. S. 700 1 $aMARQUES, J. de L. 700 1 $aVITOLA, H. R. S. 700 1 $aSILVA, W. P. da 700 1 $aDUVAL, E. H. 700 1 $aFIORENTINI, A. M. 773 $tFood Science and Technology$gv. 141, 110954, Apr. 2021.
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Embrapa Pecuária Sul (CPPSUL) |
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Registros recuperados : 118 | |
1. | | NALERIO, E. S. A carne e o sabor. Angus@News, Porto Alegre, v. 14, n. 64, p. 8, set./out. 2013.Tipo: Artigo de Divulgação na Mídia |
Biblioteca(s): Embrapa Pecuária Sul. |
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10. | | KIPPER, D.; NALERIO, E. S.; GIONGO, C.; CARDOSO, F. F. Análise sensorial mediante teste triangular da carne de bovinos submetidos a diferentes sistemas de terminação. In: SALÃO INTERNACIONAL DE ENSINO, EXTENSÃO E PESQUISA, 5., 2013, Bagé. Discussão, participação e cidadania: anais. Bagé: Unipampa, 2013. SIEPE.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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15. | | GIONGO, C.; NALERIO, E. S.; DIAS, L. B.; BORBA, M. F. S.; FISCHER, D. C. Características de carcaça e da carne de ovinos da raça Texel com diferentes idades de abate. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida: anais. Gramado: SBCTA Regional, 2016. Food: the tree that sustains life. 1575.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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19. | | ANDRADE, J. C.; SOBRAL, L. A.; NALERIO, E. S.; BARCELLOS, M. D.; ARES, G.; DELIZA, R. Identificando oportunidades para o mercado brasileiro de carne ovina de acordo com o consumidor. In: SIMPÓSIO INTERNACIONAL DE INNOVACIÓN Y DESARROLLO DE ALIMENTOS, 7.; CONGRESO IBEROAMERICANO DE INGENIERÍA DE ALIMENTOS, 10., 2015, Montevidéo. Desafíos, avances e innovación en el procesamiento de alimentos. Montevidéo: Laboratorio Tecnológico del Uruguay, 2015. 1 p. AS 327.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Pecuária Sul. |
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20. | | ANDRADE, J. C. de; NALERIO, E. S.; GIONGO, C.; BARCELLOS, M. D.; ARES, G.; DELIZA, R. Impacto das características não sensoriais na escolha de presunto cru e copa. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida: anais. Gramado: SBCTA Regional, 2016. Food: the tree that sustains life. 187.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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Registros recuperados : 118 | |
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