Data/hora: 23/06/2024 23:32:20

Biblioteca(s): 	Embrapa Pantanal.	
Data corrente: 	29/03/2004	
Data da última atualização: 	27/03/2017	
Autoria: 	INOUE-NAGATA, A. K.; ALBUQUERQUE, L. C.; ROCHA, W. B.; NAGATA, T.	
Afiliação: 	Embrapa Hortalicas (Brasilia, DF). Universidade Catolica de Brasilia (Brasilia, DF).	
Título: 	A simple method for cloning the complete begomovirus genome using the bacteriophage phi 29 DNA polymerase.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	Journal of Virological Methods, v.116, p.209-211, 2004.	
Idioma: 	Inglês	
Conteúdo: 	The bacteriophage oDNA polymerase amplifies circular DNA in a rolling circle amplifications mechanism. This characteristic was applied to amplify and clone the complete circular DNA genome of a begomovirus. Total DNA extracted from infected tissue was used as the template of an amplification reaction using the commercial kit TempliPhi (Amersham Biosciences). The amplified DNA could be used for direct sequencing and was cloned after digestion with a single cutting restriction endonuclease. The use of this enzyme simplified the cloning steps and increase the cloning efficiency of the complete genome of a circular plant DNA virus.	
Palavras-Chave: 	Rolling circle.	
Thesagro: 	Genoma.	
Thesaurus Nal: 	circular DNA; Geminiviridae; genome.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pantanal (CPAP)	
Exemplares: 	CPAP - (53858-1) - ADD - SP - PP - SP16532 -  - 16532 - DIS