Data/hora: 17/06/2024 10:16:50

Biblioteca(s): | Embrapa Hortaliças.
Data corrente: | 04/04/2002
Data da última atualização: | 31/01/2024
Autoria: | NAGATA, T.; INOUE-NAGATA, A. K.; LENT, J. van; GOLDBACH, R.; PETERS, D.
Afiliação: | TATATSUYA NAGATA, Department of Virology, Wageningen Agricultural University; ALICE KAZUKO INOUE NAGATA, CNPH; JAN VAN LENT, Department of Virology, Wageningen Agricultural University; ROB GOLDBACH, Department of Virology, Wageningen Agricultural University; DICK PETERS, Department of Virology, Wageningen Agricultural University.
Título: | Factors determining vector competence and specificity for transmission of tomato spotted wilt virus.
Ano de publicação: | 2002
Fonte/Imprenta: | Journal of General Virology, v. 83, n. 3, p. 663-671, mar. 2002.
ISSN: | 1465-2099
DOI: | 10.1099/0022-1317-83-3-663
Idioma: | Inglês
Conteúdo: | The competence of a Frankliniella occidentalis and a Thrips tabaci population to transmit Tomato spotted wilt virus (TSWV) was analysed. Adults of the F. occidentalis population transmitted this virus efficiently, whereas those of the thelytokous T. tabaci population failed to transmit. TSWV replicated in the midgut of the larvae of both populations after ingestion of virus; however, lower amounts accumulated in T. tabaci larvae than in F. occidentalis larvae. The virus was almost undetectable in T. tabaci adults, whereas high titres were readily detected in the F. occidentalis adults. The first infections in F. occidentalis larvae were detected by immunocytochemical studies in midgut epithelial and subsequently in midgut muscle cells, the ligaments, and finally in the salivary glands. The infections were weaker in the midgut epithelial and muscle cells of T. tabaci larvae, followed by an almost complete absence of any infection in the ligaments, and a complete absence in the salivary glands. Studies by electron microscopy revealed the budding of some virus particles from the basal membrane of midgut epithelial cells of F. occidentalis larvae into the extracellular space of the basal labyrinth. Enveloped virus particles were also seen in midgut muscle cells of F. occidentalis larvae. They were not discerned in epithelial and muscle cells of T. tabaci larvae and adults. This study showed that the rate of virus replication in the midgut and the extent of virus migration from the midgut to the visceral muscle cells and the salivary glands are probably crucial factors in the determination of vector competence.
Palavras-Chave: | Frankliniella ocidentalis; Insect; Transmissão; Transmission; TSWV.
Thesagro: | Inseto; Vetor; Vírus.
Thesaurus Nal: | Thrips tabaci.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Hortaliças (CNPH)
Exemplares: | CNPH - (22385-1) - UPC - AP - DD - 15908 -  - 15908 - DIS