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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/04/2023 |
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Data da última atualização: |
19/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
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DOI: |
http://dx.doi.org/10.4238/gmr19118 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
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Thesagro: |
Diagnostico. |
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Thesaurus Nal: |
Senecavirus. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500
001 2153239
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI
100 1 $aRIEGER, J. S. G.
245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis.
650 $aSenecavirus
650 $aDiagnostico
700 1 $aMANTOVANI, C.
700 1 $aSUNIGA, P. A. P.
700 1 $aPINTO, I. B.
700 1 $aSOUSA, G. A. A.
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSANTOS, L. R.
700 1 $aARAÚJO, F. R.
700 1 $aZANELLA, J. R. C.
773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 11 | |
| 2. |  | GOMES, J. da S.; SANTOS, L. R. dos; COELHO, M. B.; BERTOLACCI, M. A. B. C.; MANTOVANI, C.; SANTANA, M.; SANTOS, M. G. dos; ROSINHA, G. M. S. Avaliação da imunogenicidade e potencial de proteção de vacinas de subunidades de Brucella abortus. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 15., 2019, Campo Grande, MS. [Resumos dos trabalhos...]. Brasília, DF: Embrapa, 2019 80 p. (Embrapa Gado de Corte. Documentos, 264). Comitê Organizador: Marlene de Barros Coelho; Lenita Ramires dos Santos; Rodrigo Carvalho Alva; Lucimara Chiari; Thais Basso Amaral. p. 48-49| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | VERBISCK, N. V.; SUNIGA, P. A.; MANTOVANI, C.; SOUZA, I. I.; SANTOS, M. G. dos; ARAUJO, F. R.; SANTOS, L. R. dos. Espectro de massas de referência para identificação de Burkholderia mallei por espectrometria de massas MALDI-TOF. Campo Grande, MS: Embrapa Gado de Corte, 2022. (Embrapa Gado de Corte. Comunicado Técnico, 165). ODS 3.| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | ROSINHA, G. M. S.; SANTOS, L. R. dos; ELISEI, I.; SANCHES, C. C.; BERTOLACCI, M. A. B. C.; MANTOVANI, C.; ELISEI, C.; SOARES, C. O. Identificação de Brucella spp. em bovinos com lesões sugestivas de brucelose. Campo Grande, MS: Embrapa Gado dce Corte, 2019. 30 p. (Embrapa Gado de Corte. Boletim de Pesquisa e Desenvolvimento, 43).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | GASPAR, E. B.; SANTOS, L. R. dos; EGITO, A. A. do; SANTOS, M. G. dos; MANTOVANI, C.; RIEGER, J. DA S. G.; ABRANTES, G. A. DE S.; SUNIGA, P. A. P.; FAVACHO, J. DE M.; PINTO, I. B.; NASSAR, A. F. DE C.; SANTOS, F. L. DOS; ARAUJO, F. R. Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice. Microorganisms, v. 11, issue 10, e2597, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Pecuária Sul. |
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| 8. | | ARAUJO, F. R.; SANTOS, L. R. dos; ARECO, A. E. T.; MANTOVANI, C.; RIEGER, J. da S. G.; BORGES, F. de A.; FAVACHO, A. R. de M.; LOPES, W. D. Z.; CANCADO, P. H. D.; CASTRO, B. G. de; NAKATANI, M. T. M. Cisticercose bovina no Brasil: velho problema, novos desafios. Campo Grande, MS: Embrapa Gado de Corte, 2023. 17 p. (Embrapa Gado de Corte. Documentos, 309). ODS 9.| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; DÁVILA, A. M. R.; ZIMPEL, C. K.; ZERPA, M. C. S.; CHIEBAO, D. P.; GUIMARÃES, A. M. DE S.; NASSAR, A. F. DE C.; ARAUJO, F. R. Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing. Pathogens, v. 12, e1250, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; RIEGER, J. S. G.; GASPAR, E. B.; SANTOS, F. L. dos; MOTA, R. A.; CHAVES, K. P.; EGITO, A. A.; O. FILHO, J. C.; NASSAR, A. F. C.; SANTOS, L. R. dos; ARAUJO, F. R. Molecular detection of Burkholderia mallei in different geographic regions of Brazil. Brazilian Journal of Microbiology, v. 54, n. 2, p. 1275-1285, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sul. |
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| 11. | | SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G.; RIEGER, J. S. G.; GASPAR, E. B.; SANTOS, F. L. DOS; MOTA, R. A.; CHAVES, K. P.; EGITO, A. A. do; OLIVEIRA FILHO, J. C. DE; NASSAR, A. F. DE C.; SANTOS, L. R. dos; ARAUJO, F. R. Molecular detection of Burkholderia mallei in different geographic regions of Brazil. Brazilian Journal of Microbiology, v. 54, issue 2, p. 1275-1285, 2023. Na publicação: José Carlos O. Filho; Alessandra F. Castro Nassar| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Pecuária Sul. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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