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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/01/2001 |
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Data da última atualização: |
05/01/2001 |
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Autoria: |
RITCHIE, N. J.; SCHUTTER, M. E.; DICK, R. P.; MYROLD, D. D. |
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Título: |
Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, p. 1668-1675, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition. MenosIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02152naa a2200157 a 4500
001 1621188
005 2001-01-05
008 2000 bl --- 0-- u #d
100 1 $aRITCHIE, N. J.
245 $aUse of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil.
260 $c2000
520 $aIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition.
700 1 $aSCHUTTER, M. E.
700 1 $aDICK, R. P.
700 1 $aMYROLD, D. D.
773 $tApplied and Environmental Microbiology, Washington$gv. 66, p. 1668-1675, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
16/08/1993 |
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Data da última atualização: |
30/05/2007 |
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Autoria: |
BARREIRO NETO, M.; COSTA, J. N. da; CAVALCANTI, F. B.; FREIRE, E. C.; CARVALHO, L. P.; VASCONCELOS, M. F. |
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Título: |
Competicao de cultivares de algodoeiro herbaceo (Gossypium hirsutum L.r. latifolium) com tipos locais no Estado da Paraiba. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Campina Grande: EMBRAPA-CNPA, 1982. |
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Páginas: |
15p. |
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Série: |
(EMBRAPA.CNPA. Botetim de Pesquisa, 5). |
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Idioma: |
Português |
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Conteúdo: |
Objetivando verificar a possibilidade de cultivo do algodoeiro herbáceo em vales do Sertão onde a aridez é menos acentuada, foram conduzidos experimentos durante dois anos em vinte localidades. Os resultados indicam que é recomendável promover a substituição dos tipos locais de fibras indesejável por cultivares herbáceas adaptadas à zona semi-árida, sem que se esteja aumentando o nível de risco econômico, desde que a substituição se proceda nos baixios aluviais dos vales dos rios. |
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Palavras-Chave: |
Annual cotton; Brasil; Cultivo; Paraiba. |
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Thesagro: |
Algodão Herbáceo; Gossypium Hirsutum; Melhoramento. |
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Thesaurus NAL: |
breeding. |
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Categoria do assunto: |
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A Sistemas de Cultivo |
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Marc: |
LEADER 01329nam a2200289 a 4500
001 1262536
005 2007-05-30
008 1982 bl uuuu u0uu1 u #d
100 1 $aBARREIRO NETO, M.
245 $aCompeticao de cultivares de algodoeiro herbaceo (Gossypium hirsutum L.r. latifolium) com tipos locais no Estado da Paraiba.
260 $aCampina Grande: EMBRAPA-CNPA$c1982
300 $a15p.
490 $a(EMBRAPA.CNPA. Botetim de Pesquisa, 5).
520 $aObjetivando verificar a possibilidade de cultivo do algodoeiro herbáceo em vales do Sertão onde a aridez é menos acentuada, foram conduzidos experimentos durante dois anos em vinte localidades. Os resultados indicam que é recomendável promover a substituição dos tipos locais de fibras indesejável por cultivares herbáceas adaptadas à zona semi-árida, sem que se esteja aumentando o nível de risco econômico, desde que a substituição se proceda nos baixios aluviais dos vales dos rios.
650 $abreeding
650 $aAlgodão Herbáceo
650 $aGossypium Hirsutum
650 $aMelhoramento
653 $aAnnual cotton
653 $aBrasil
653 $aCultivo
653 $aParaiba
700 1 $aCOSTA, J. N. da
700 1 $aCAVALCANTI, F. B.
700 1 $aFREIRE, E. C.
700 1 $aCARVALHO, L. P.
700 1 $aVASCONCELOS, M. F.
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