Data/hora: 01/06/2024 13:01:38

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 01/08/1992
Data da última atualização: | 09/01/2024
Autoria: | FUJISAKI, Y.; MURAKAMI, Y.; SUSUKI, H.
Título: | Establihsment of an attenuated strain of porcinar parvovirus by serial passage at low temperature.
Ano de publicação: | 1982
Fonte/Imprenta: | National Institute of Animal Health Quartely, v. 22, n. 1, p. 1-7, 1982.
Idioma: | Inglês
Conteúdo: | Abstract: To prepare a live virus vaccine strain for the prevention of porcine parvovirus infection, the 90HS strain, isolated from the brain of a stillborn porcine fetus, was subjected to the first 45 serial passages in swine kidney established (ESK) cells of porcine kidney origin at 30-35 degrees C and to the 46th and later serial passages in the same cells as these at 32 degrees C. When swine were inoculated with the strain at the 38th passage level possessing such properties as expressed with rct/37+ and rct/40-, they presented viremia, virus discharge, and the transmission of virus to other swine. When swine were inoculated with the strain at the 54th and 55th passage level possessing such properties as expressed with rct/37- and rct/40-, they failed to exhibit viremia, virus discharge, and the transmission of virus to other swine, but retained for a long time hemagglutination-inhibiting antibody which had been produced after inoculation. A low virulent variant strain was obtained after 54 serial passages at low temperature. It was called the HT- strain.
Palavras-Chave: | Attenuated vaccines; Methods Vaccines; Parvovirus.
Thesagro: | Vacina.
Thesaurus Nal: | Antibody formation; Immunology; Live vaccines; Parvoviridae; Swine; Temperature; Vaccination; Viral vaccines.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (7540-1) - ADD - AP - PP - SP 04941 -  - SP 04941 - DIS