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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/04/2004 |
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Data da última atualização: |
16/04/2004 |
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Autoria: |
MUNYIKWA, T. R. I; RAEMAKERS, C. C. J. M; SCHREUDER, M; KREUZE, J; SUURS, L; KOK, R; ROZEBOOM, M; JACOBSEN, E; VISSER, R. G. F. |
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Título: |
Introduction and expression of antisense adpg-pyrophos-phorylase of cassava leads to decreased levels of starch and increased levels of sugars. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.62, nov, 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: From a cassva tuber cDNA library full lenght AGPase B cDNA's were isolated and characterised. From these, one cDNA encoding the small subunit of AGPase (B45-1) was used to make antisense constructs suitable for Agrobacterium and particle bombardment transformation. In the binary vector the AGPase gene was under control the 35S cauliflower mosaic prometer and selection was obtained by the NPT-II gene conferring resistance to kanamtcin. In the vector used for parcicle bombardment the AGPase antisense gene was under control of the double 35S promoter and selection was based on the activity of the luciferase gene. The Agrobacterium construct was used to introduce the antisense AGPase in potato where the effect was determined in tubers. From the 250 transformants about 20% showed an effect in that they had on overage four times as many tubers per plant. In total 6% of the transformants showed a dramatic reduction in AGPase mRNA level as well as starch content. In these plants the level of soluble sugars was increased with a factor five. The particle bombardment construct was used in transformation experiments with cassava friable embryogenic cultures. In total 24 independent cassava transformants were obtained. These plants were grown in vitro on high sucrose medium to stimulate thickend stems. Three of these transformants showed a severe phenotype in thickend stems of in vitro plants. Expression of the AGPase B antisense gene in these plants resulted in cassava plants with extremely low levels of starch, as compared to control plants, as shown by iodine staining of the thickened stems. AII the transgenic cassava plants were transfrred to the greenhouse. Results regarding the effect of the antisense AGPAase inhibition in tuberous roots of these plants will be presented and discussed. MenosAbstract: From a cassva tuber cDNA library full lenght AGPase B cDNA's were isolated and characterised. From these, one cDNA encoding the small subunit of AGPase (B45-1) was used to make antisense constructs suitable for Agrobacterium and particle bombardment transformation. In the binary vector the AGPase gene was under control the 35S cauliflower mosaic prometer and selection was obtained by the NPT-II gene conferring resistance to kanamtcin. In the vector used for parcicle bombardment the AGPase antisense gene was under control of the double 35S promoter and selection was based on the activity of the luciferase gene. The Agrobacterium construct was used to introduce the antisense AGPase in potato where the effect was determined in tubers. From the 250 transformants about 20% showed an effect in that they had on overage four times as many tubers per plant. In total 6% of the transformants showed a dramatic reduction in AGPase mRNA level as well as starch content. In these plants the level of soluble sugars was increased with a factor five. The particle bombardment construct was used in transformation experiments with cassava friable embryogenic cultures. In total 24 independent cassava transformants were obtained. These plants were grown in vitro on high sucrose medium to stimulate thickend stems. Three of these transformants showed a severe phenotype in thickend stems of in vitro plants. Expression of the AGPase B antisense gene in these plants resulted in cassava plants ... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02502naa a2200217 a 4500
001 1651922
005 2004-04-16
008 1998 bl uuuu u00u1 u #d
100 1 $aMUNYIKWA, T. R. I
245 $aIntroduction and expression of antisense adpg-pyrophos-phorylase of cassava leads to decreased levels of starch and increased levels of sugars.
260 $c1998
520 $aAbstract: From a cassva tuber cDNA library full lenght AGPase B cDNA's were isolated and characterised. From these, one cDNA encoding the small subunit of AGPase (B45-1) was used to make antisense constructs suitable for Agrobacterium and particle bombardment transformation. In the binary vector the AGPase gene was under control the 35S cauliflower mosaic prometer and selection was obtained by the NPT-II gene conferring resistance to kanamtcin. In the vector used for parcicle bombardment the AGPase antisense gene was under control of the double 35S promoter and selection was based on the activity of the luciferase gene. The Agrobacterium construct was used to introduce the antisense AGPase in potato where the effect was determined in tubers. From the 250 transformants about 20% showed an effect in that they had on overage four times as many tubers per plant. In total 6% of the transformants showed a dramatic reduction in AGPase mRNA level as well as starch content. In these plants the level of soluble sugars was increased with a factor five. The particle bombardment construct was used in transformation experiments with cassava friable embryogenic cultures. In total 24 independent cassava transformants were obtained. These plants were grown in vitro on high sucrose medium to stimulate thickend stems. Three of these transformants showed a severe phenotype in thickend stems of in vitro plants. Expression of the AGPase B antisense gene in these plants resulted in cassava plants with extremely low levels of starch, as compared to control plants, as shown by iodine staining of the thickened stems. AII the transgenic cassava plants were transfrred to the greenhouse. Results regarding the effect of the antisense AGPAase inhibition in tuberous roots of these plants will be presented and discussed.
700 1 $aRAEMAKERS, C. C. J. M
700 1 $aSCHREUDER, M
700 1 $aKREUZE, J
700 1 $aSUURS, L
700 1 $aKOK, R
700 1 $aROZEBOOM, M
700 1 $aJACOBSEN, E
700 1 $aVISSER, R. G. F.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.62, nov, 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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