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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
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Data corrente: |
20/04/2023 |
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Data da última atualização: |
18/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S. |
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Afiliação: |
Universidade de São Paulo; Universidade Federal de São Carlos; Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; DANIEL SOUZA CORREA, CNPDIA. |
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Título: |
Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Polymer Research, v. 30, 166, 2023. |
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DOI: |
https://doi.org/10.1007/s10965-023-03535-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Polymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus MenosPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides,... Mostrar Tudo |
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Palavras-Chave: |
Antibiotic release; Electrospinning; Eletrofiação; Mastite; Slow-release. |
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Thesagro: |
Antibiótico; Bovino; Doença Animal; Tratamento. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02915naa a2200301 a 4500
001 2153293
005 2023-07-18
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10965-023-03535-7$2DOI
100 1 $aPEREA, G. N. R.
245 $aElectrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin$binvestigation of morphological features, antibiotic release and antimicrobial properties.$h[electronic resource]
260 $c2023
520 $aPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus
650 $aAntibiótico
650 $aBovino
650 $aDoença Animal
650 $aTratamento
653 $aAntibiotic release
653 $aElectrospinning
653 $aEletrofiação
653 $aMastite
653 $aSlow-release
700 1 $aPAVINATTO, A.
700 1 $aSCHNEIDER, R.
700 1 $aMUNK, M.
700 1 $aBRANDAO, H. de M.
700 1 $aCORREA, D. S.
773 $tJournal of Polymer Research$gv. 30, 166, 2023.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 43 | |
| 2. |  | MAZA, A. P.; MUNK, M.; ONORATO, G. C.; NEVES, C. C.; BRANDAO, H. de M.; CAMPOS, J. M. S. Avaliação da genotoxicidade de nanopartículas de titanato de bário em Alium cepa In: SIMPÓSIO DE ECOTOXICOLOGIA, 3., 2016, Rio Claro. Anais... Rio Claro: Universidade Estadual Paulista, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | ZANETTE, R.; ALMEIDA, L. B. F.; ALMEIDA, C. G.; OLIVEIRA, C. R. de; BRANDÃO, H. M.; MUNK, M. Análise da citoxicidade in vitro das nanofibras de celulose de algodão. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p.681-684. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | ZANETTE, R. S. S.; ALMEIDA, L. B. F. De; BRANDAO, H. de M.; MARANDUBA, M. Da C.; MUNK, M. Avaliação in vitro da biocompatibilidade de nanofibras de celulose em cultura de células-tronco da polpa dentária humana In: SIMPÓSIO NACIONAL DE NANOBIOTECNOLOGIA, 1.; WORKSHOP DE NANOBIOTECNOLOGIA DA UFMG, 2., 2016, Belo Horizonte. Avanços e aplicações: anais. Belo Horizonte: Universidade Federal de Minas Gerais, 2016. p. 82.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S. Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties. Journal of Polymer Research, v. 30, 166, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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| 9. | | SANTOS, E. M. dos; TAVARES, L. S.; FAYER, L.; BRANDAO, H. de M.; MUNK, M.; SANTOS, M. de O. Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations. Archives of Microbiology, v. 204, n. 3, 184, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | QUINTAO, C. C. R.; SARAIVA, N. Z.; OLIVEIRA, C. S.; CAMARGO, L. S. de A.; MUNK, M. Zinc oxide nanoparticles supplementation on in vitro maturation of bovine oocytes and its effects on the embryo quality, lipid accumulation, and preimplantation development. Animal Reproduction, v. 20, n. 2, 2023. Abstracts of 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | SIQUEIRA, J. T. da C.; REIS, A. C.; LOPES, J. M. L.; LADEIRA, L. O.; VICCINI, L. F.; BRANDAO, H. de M.; MUNK, M.; SOUSA, S. M. de. Chromosomal aberrations and changes in the methylation patterns of Lactuca sativa L. (Asteraceae) exposed to carbon nanotubes. Biologia, v. 78, p. 1991-2002, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | GLANZMANN, D.; FAYER, L.; ZANETTE, R. S. S.; GERN, J. C.; OLIVEIRA, L. F. C. de; MUNK, M.; BRANDAO, H. de M. Caracterização físico-química de nanopartículas de dióxido de titânio para produção de nanocompósitos. In: PASSOS, L. P. (ed.). Coletânea de Iniciação Científica da Embrapa Gado de Leite-PIBIC CNPq 2020-2021. Juiz de Fora: Embrapa Gado de Leite, 2021. (Embrapa Gado de Leite. Documentos, 262).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | QUINTAO, C. C. R.; ALMEIDA, C. G. de; SOUZA, G. T.; LADEIRA, L. O.; BRANDAO, H. de M.; CAMARGO, L. S. de A.; MUNK, M. Complexação da proteína CAS9 e RNA-GUIA com nanotubos de carbono visando edição gênica em células e embriões de mamíferos. In: SIMPÓSIO NACIONAL DE NANOBIOTECNOLOGIA, 2., 2019, São Bernardo do Campo. Livro de resumos. Santo André: Universidade Federal do ABC, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | OLIVEIRA, E. R.; FAYER, L.; ZANETTE, R. S. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; MARANDUBA, C. M. C.; BRANDAO, H. de M.; MUNK, M. Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth. Nanotechnology, v. 33, n. 6, 065101, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | MUNK, M.; BRANDÃO, H. M.; NOWAK, S.; MOUTON, L.; GERN, J. C.; GUIMARÃES, A. S.; YÉPRÉMIAN, C.; COUTÉ, A.; RAPOSO, N. R. B.; MARCONCINI, J. M.; BRAYNER, R. Direct and indirect toxic effects of cotton-derived cellulose nanofibers on filamentous green algae. Ecotoxicology and environmental safety, v. 122, p. 399-405, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. Na publicação: Luiz Sergio Almeida Camargo.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | AMARAL, D. L. A. S. Do; ALMEIDA, L. B. F. De; ZANETTE, R. De S. S.; BRANDAO, H. de M.; MARANDUBA, C. M. Da C.; MUNK, M. Produção de hidrogéis de alginato com nanopartículas de titanato de bário para uso na engenharia de tecido ósseo In: SIMPÓSIO NACIONAL DE NANOBIOTECNOLOGIA, 1.; WORKSHOP DE NANOBIOTECNOLOGIA DA UFMG, 2., 2016, Belo Horizonte. Avanços e aplicações: anais. Belo Horizonte: Universidade Federal de Minas Gerais, 2016. p. 33-34.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 43 | |
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