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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
23/01/2006 |
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Data da última atualização: |
21/08/2015 |
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Autoria: |
SAMAHA, R. R.; BOYLE, T. H.; MULCAHY, D. L. |
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Título: |
Self-incompatibility of Zinnia angustifolia HBK (Compositae): I. Aplication of visible light and fluorescence microscopy for assessment of self-incompatibility. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Sexual Plant Reproduction, Heidleberg, v.2, n.1, p.18-26, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Visible light and UV epifluorescence microscopy were used to assess self-incompatibility (SI) in 3 Z. angustifolia clones. Pistils were fixed 24 h after pollination and stained with either aniline blue in lactophenol (visible light microscopy) or decolorized aniline blue (fluorescence microscopy). Percentage of florets with embryos 21 days after pollination (percentage embryo set) was used as a control. Embryo set following self- or incompatible cross-pollinations ranged from 0 to 9.9% whereas compatible crosses yielded 55.5-87.1% embryo set. Observations using visible light microscopy indicated that pollen load and number of germinated grains were significantly higher for compatible compared to incompatible crosses, and both variables were positively correlated (r = 0.89-0.96) to percentage embryo set. Examination with UV epifluorescence microscopy revealed that pollen load was higher and little or no callose accumulated in stigmatic papillae following compatible crosses, whereas in incompatible crosses pollen load was low and callose lenticules were deposited in stigmatic papillae; the correlation between pollen load and percentage embryo set was 0.89. The intensity of callose fluorescence of the pollen tube-papillae attachment sites was quantitatively measured via microspectrofluorometry. Callose fluorescence intensity ranged from 47.9 to 62.6% for incompatible and from 6.4 to 9.9% for compatible crosses, and was negatively correlated (r = -0.95) with percentage embryo set. MenosVisible light and UV epifluorescence microscopy were used to assess self-incompatibility (SI) in 3 Z. angustifolia clones. Pistils were fixed 24 h after pollination and stained with either aniline blue in lactophenol (visible light microscopy) or decolorized aniline blue (fluorescence microscopy). Percentage of florets with embryos 21 days after pollination (percentage embryo set) was used as a control. Embryo set following self- or incompatible cross-pollinations ranged from 0 to 9.9% whereas compatible crosses yielded 55.5-87.1% embryo set. Observations using visible light microscopy indicated that pollen load and number of germinated grains were significantly higher for compatible compared to incompatible crosses, and both variables were positively correlated (r = 0.89-0.96) to percentage embryo set. Examination with UV epifluorescence microscopy revealed that pollen load was higher and little or no callose accumulated in stigmatic papillae following compatible crosses, whereas in incompatible crosses pollen load was low and callose lenticules were deposited in stigmatic papillae; the correlation between pollen load and percentage embryo set was 0.89. The intensity of callose fluorescence of the pollen tube-papillae attachment sites was quantitatively measured via microspectrofluorometry. Callose fluorescence intensity ranged from 47.9 to 62.6% for incompatible and from 6.4 to 9.9% for compatible crosses, and was negatively correlated (r = -0.95) with percentage embryo se... Mostrar Tudo |
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Palavras-Chave: |
Auto incompatibilidade; Calose; Compatibility; Microscopia de fluorescencia; Ornamental herbaceous plants; Self incompatibility. |
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Thesagro: |
Pólen. |
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Thesaurus Nal: |
breeding; ornamental plants; pollination. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02337naa a2200265 a 4500
001 1278638
005 2015-08-21
008 1989 bl --- 0-- u #d
100 1 $aSAMAHA, R. R.
245 $aSelf-incompatibility of Zinnia angustifolia HBK (Compositae)$bI. Aplication of visible light and fluorescence microscopy for assessment of self-incompatibility.
260 $c1989
520 $aVisible light and UV epifluorescence microscopy were used to assess self-incompatibility (SI) in 3 Z. angustifolia clones. Pistils were fixed 24 h after pollination and stained with either aniline blue in lactophenol (visible light microscopy) or decolorized aniline blue (fluorescence microscopy). Percentage of florets with embryos 21 days after pollination (percentage embryo set) was used as a control. Embryo set following self- or incompatible cross-pollinations ranged from 0 to 9.9% whereas compatible crosses yielded 55.5-87.1% embryo set. Observations using visible light microscopy indicated that pollen load and number of germinated grains were significantly higher for compatible compared to incompatible crosses, and both variables were positively correlated (r = 0.89-0.96) to percentage embryo set. Examination with UV epifluorescence microscopy revealed that pollen load was higher and little or no callose accumulated in stigmatic papillae following compatible crosses, whereas in incompatible crosses pollen load was low and callose lenticules were deposited in stigmatic papillae; the correlation between pollen load and percentage embryo set was 0.89. The intensity of callose fluorescence of the pollen tube-papillae attachment sites was quantitatively measured via microspectrofluorometry. Callose fluorescence intensity ranged from 47.9 to 62.6% for incompatible and from 6.4 to 9.9% for compatible crosses, and was negatively correlated (r = -0.95) with percentage embryo set.
650 $abreeding
650 $aornamental plants
650 $apollination
650 $aPólen
653 $aAuto incompatibilidade
653 $aCalose
653 $aCompatibility
653 $aMicroscopia de fluorescencia
653 $aOrnamental herbaceous plants
653 $aSelf incompatibility
700 1 $aBOYLE, T. H.
700 1 $aMULCAHY, D. L.
773 $tSexual Plant Reproduction, Heidleberg$gv.2, n.1, p.18-26, 1989.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/08/2003 |
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Data da última atualização: |
10/09/2014 |
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Autoria: |
FERREIRA, A. de M.; SÁ, W. F. de. (Ed.). |
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Afiliação: |
Embrapa Gado de Leite. |
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Título: |
Anais do Workshop sobre embriões bovinos produzidos in vitro: perspectivas de utilização e possíveis impactos na pecuária brasileira. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Juiz de Fora: Embrapa Gado de Leite, 2002. |
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Páginas: |
91 p. |
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Série: |
(Embrapa Gado de Leite. Documentos, 88). |
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Idioma: |
Português |
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Conteúdo: |
Eficiência, limitações e perspectivas futuras na produção in vitro de embriões bovinos; Eficiência, problemas encontrados e perspectivas futuras no uso de embriões bovinos produzidos in vitro; Sexagem de espermatozóides e de embriões; Definição dos pontos de estrangulamento na técnica de produção in vitro de embriões. |
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Palavras-Chave: |
Bovinos - embriões in vitro - utilização; pecuária brasileira - embriões bovinos in vitro - impacto; PIVE; Tranferência de embriões. |
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Thesagro: |
Gado de Corte; Produção Animal; Reprodução. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82562/1/Anais-do-workshop.pdf
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Marc: |
LEADER 01132nam a2200229 a 4500
001 1594812
005 2014-09-10
008 2002 bl uuuu 00u1 u #d
100 1 $aFERREIRA, A. de M.
245 $aAnais do Workshop sobre embriões bovinos produzidos in vitro$bperspectivas de utilização e possíveis impactos na pecuária brasileira.
260 $aJuiz de Fora: Embrapa Gado de Leite$c2002
300 $a91 p.
490 $a(Embrapa Gado de Leite. Documentos, 88).
520 $aEficiência, limitações e perspectivas futuras na produção in vitro de embriões bovinos; Eficiência, problemas encontrados e perspectivas futuras no uso de embriões bovinos produzidos in vitro; Sexagem de espermatozóides e de embriões; Definição dos pontos de estrangulamento na técnica de produção in vitro de embriões.
650 $aGado de Corte
650 $aProdução Animal
650 $aReprodução
653 $aBovinos - embriões in vitro - utilização
653 $apecuária brasileira - embriões bovinos in vitro - impacto
653 $aPIVE
653 $aTranferência de embriões
700 1 $aSÁ, W. F. de. (Ed.).
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Embrapa Gado de Leite (CNPGL) |
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